Some aspects of erythrocyte preparation for AFM-study

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

The features of AFM studies of erythrocytes are discussed in this paper. It is shown the influence of the chemical composition of the buffer and centrifugation parameters on the morphology of the initial cells. The effects of aging and dehydration of erythrocytes on their structural integrity are investigated. Practical recommendations of the sample preparation for AFM studies are given. The possible errors in the interpretation of the obtained results are demonstrated. Artifacts that can appear in AFM images are described.

Original language	English
Title of host publication	AIP Conference Proceedings
Editors	Victor E. Panin, Sergey G. Psakhie, Vasily M. Fomin, Victor E. Panin, Sergey G. Psakhie
Publisher	American Institute of Physics Inc.
Pages	307-310
Number of pages	4
Volume	1623
ISBN (Electronic)	9780735412606
DOIs	https://doi.org/10.1063/1.4898943
Publication status	Published - 1 Jan 2014
Event	International Conference on Physical Mesomechanics of Multilevel Systems 2014 - Tomsk, Russian Federation Duration: 3 Sep 2014 → 5 Sep 2014

Conference

Conference	International Conference on Physical Mesomechanics of Multilevel Systems 2014
Country	Russian Federation
City	Tomsk
Period	3.9.14 → 5.9.14

Fingerprint

erythrocytes

atomic force microscopy

preparation

recommendations

dehydration

integrity

artifacts

chemical composition

buffers

cells

ASJC Scopus subject areas

Physics and Astronomy(all)

Cite this

Kozelskaya, A. I., & Panin, A. V. (2014). Some aspects of erythrocyte preparation for AFM-study. In V. E. Panin, S. G. Psakhie, V. M. Fomin, V. E. Panin, & S. G. Psakhie (Eds.), AIP Conference Proceedings (Vol. 1623, pp. 307-310). American Institute of Physics Inc.. https://doi.org/10.1063/1.4898943

Some aspects of erythrocyte preparation for AFM-study. / Kozelskaya, A. I.; Panin, A. V.

AIP Conference Proceedings. ed. / Victor E. Panin; Sergey G. Psakhie; Vasily M. Fomin; Victor E. Panin; Sergey G. Psakhie. Vol. 1623 American Institute of Physics Inc., 2014. p. 307-310.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Kozelskaya, AI & Panin, AV 2014, Some aspects of erythrocyte preparation for AFM-study. in VE Panin, SG Psakhie, VM Fomin, VE Panin & SG Psakhie (eds), AIP Conference Proceedings. vol. 1623, American Institute of Physics Inc., pp. 307-310, International Conference on Physical Mesomechanics of Multilevel Systems 2014, Tomsk, Russian Federation, 3.9.14. https://doi.org/10.1063/1.4898943

Kozelskaya AI , Panin AV. Some aspects of erythrocyte preparation for AFM-study. In Panin VE, Psakhie SG, Fomin VM, Panin VE, Psakhie SG, editors, AIP Conference Proceedings. Vol. 1623. American Institute of Physics Inc. 2014. p. 307-310 https://doi.org/10.1063/1.4898943

Kozelskaya, A. I. ; Panin, A. V. / Some aspects of erythrocyte preparation for AFM-study. AIP Conference Proceedings. editor / Victor E. Panin ; Sergey G. Psakhie ; Vasily M. Fomin ; Victor E. Panin ; Sergey G. Psakhie. Vol. 1623 American Institute of Physics Inc., 2014. pp. 307-310

@inproceedings{197c3e3fb7084cf29b7f8bb9c95b426f,

title = "Some aspects of erythrocyte preparation for AFM-study",

abstract = "The features of AFM studies of erythrocytes are discussed in this paper. It is shown the influence of the chemical composition of the buffer and centrifugation parameters on the morphology of the initial cells. The effects of aging and dehydration of erythrocytes on their structural integrity are investigated. Practical recommendations of the sample preparation for AFM studies are given. The possible errors in the interpretation of the obtained results are demonstrated. Artifacts that can appear in AFM images are described.",

author = "Kozelskaya, {A. I.} and Panin, {A. V.}",

year = "2014",

month = "1",

day = "1",

doi = "10.1063/1.4898943",

language = "English",

volume = "1623",

pages = "307--310",

editor = "Panin, {Victor E.} and Psakhie, {Sergey G.} and Fomin, {Vasily M.} and Panin, {Victor E.} and Psakhie, {Sergey G.}",

booktitle = "AIP Conference Proceedings",

publisher = "American Institute of Physics Inc.",

}

TY - GEN

T1 - Some aspects of erythrocyte preparation for AFM-study

AU - Kozelskaya, A. I.

AU - Panin, A. V.

PY - 2014/1/1

Y1 - 2014/1/1

N2 - The features of AFM studies of erythrocytes are discussed in this paper. It is shown the influence of the chemical composition of the buffer and centrifugation parameters on the morphology of the initial cells. The effects of aging and dehydration of erythrocytes on their structural integrity are investigated. Practical recommendations of the sample preparation for AFM studies are given. The possible errors in the interpretation of the obtained results are demonstrated. Artifacts that can appear in AFM images are described.

AB - The features of AFM studies of erythrocytes are discussed in this paper. It is shown the influence of the chemical composition of the buffer and centrifugation parameters on the morphology of the initial cells. The effects of aging and dehydration of erythrocytes on their structural integrity are investigated. Practical recommendations of the sample preparation for AFM studies are given. The possible errors in the interpretation of the obtained results are demonstrated. Artifacts that can appear in AFM images are described.

UR - http://www.scopus.com/inward/record.url?scp=84915779601&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84915779601&partnerID=8YFLogxK

U2 - 10.1063/1.4898943

DO - 10.1063/1.4898943

M3 - Conference contribution

VL - 1623

SP - 307

EP - 310

BT - AIP Conference Proceedings

A2 - Panin, Victor E.

A2 - Psakhie, Sergey G.

A2 - Fomin, Vasily M.

A2 - Panin, Victor E.

A2 - Psakhie, Sergey G.

PB - American Institute of Physics Inc.

ER -

Access to Document

10.1063/1.4898943