Some aspects of erythrocyte preparation for AFM-study

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Abstract

The features of AFM studies of erythrocytes are discussed in this paper. It is shown the influence of the chemical composition of the buffer and centrifugation parameters on the morphology of the initial cells. The effects of aging and dehydration of erythrocytes on their structural integrity are investigated. Practical recommendations of the sample preparation for AFM studies are given. The possible errors in the interpretation of the obtained results are demonstrated. Artifacts that can appear in AFM images are described.

Original language	English
Title of host publication	AIP Conference Proceedings
Editors	Victor E. Panin, Sergey G. Psakhie, Vasily M. Fomin, Victor E. Panin, Sergey G. Psakhie
Publisher	American Institute of Physics Inc.
Pages	307-310
Number of pages	4
Volume	1623
ISBN (Electronic)	9780735412606
DOIs	https://doi.org/10.1063/1.4898943
Publication status	Published - 1 Jan 2014
Event	International Conference on Physical Mesomechanics of Multilevel Systems 2014 - Tomsk, Russian Federation Duration: 3 Sep 2014 → 5 Sep 2014

Conference

Conference	International Conference on Physical Mesomechanics of Multilevel Systems 2014
Country	Russian Federation
City	Tomsk
Period	3.9.14 → 5.9.14

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ASJC Scopus subject areas

Physics and Astronomy(all)

Cite this

Kozelskaya, A. I., & Panin, A. V. (2014). Some aspects of erythrocyte preparation for AFM-study. In V. E. Panin, S. G. Psakhie, V. M. Fomin, V. E. Panin, & S. G. Psakhie (Eds.), AIP Conference Proceedings (Vol. 1623, pp. 307-310). American Institute of Physics Inc.. https://doi.org/10.1063/1.4898943

Kozelskaya, AI & Panin, AV 2014, Some aspects of erythrocyte preparation for AFM-study. in VE Panin, SG Psakhie, VM Fomin, VE Panin & SG Psakhie (eds), AIP Conference Proceedings. vol. 1623, American Institute of Physics Inc., pp. 307-310, International Conference on Physical Mesomechanics of Multilevel Systems 2014, Tomsk, Russian Federation, 3.9.14. https://doi.org/10.1063/1.4898943

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Access to Document

10.1063/1.4898943