Abstract
In the present study, we report a simple and efficient method of gelatin immobilization on the surface of PLA electrospun fibers using pre-treatment with a mixture of toluene and ethanol allowing to form swelled surface layer followed by gelatin adsorption from its solution in PBS. Our results demonstrate that gelatin immobilization leads to a decrease in the water contact angle from 120° to 0° enhances scaffold strength up to 50%, and doubles the number of adhered cells and their average area. We observed that the maximum amount of gelatin (0.07 ± 0.01 mg per cm3 of the scaffold) was immobilized during the first five minutes of exposure to the gelatin solution. Modified scaffolds demonstrated increased strength.
| Original language | English |
|---|---|
| Pages (from-to) | 137-140 |
| Number of pages | 4 |
| Journal | Colloids and Surfaces B: Biointerfaces |
| Volume | 177 |
| DOIs | |
| Publication status | Published - 1 May 2019 |
Keywords
- Electrospun scaffolds
- Gelatin
- Polylactic acid
- Surface modification
ASJC Scopus subject areas
- Biotechnology
- Surfaces and Interfaces
- Physical and Theoretical Chemistry
- Colloid and Surface Chemistry
Fingerprint Dive into the research topics of '“Solvent/non-solvent” treatment as a method for non-covalent immobilization of gelatin on the surface of poly(L-lactic acid) electrospun scaffolds'. Together they form a unique fingerprint.
Cite this
Goreninskii, S. I., Guliaev, R. O., Stankevich, K. S., Danilenko, N. V., Bolbasov, E. N., Golovkin, A. S., Mishanin, A. I., Filimonov, V. D., & Tverdokhlebov, S. I. (2019). “Solvent/non-solvent” treatment as a method for non-covalent immobilization of gelatin on the surface of poly(L-lactic acid) electrospun scaffolds. Colloids and Surfaces B: Biointerfaces, 177, 137-140. https://doi.org/10.1016/j.colsurfb.2019.01.060