Abstract
In the present study, we report a simple and efficient method of gelatin immobilization on the surface of PLA electrospun fibers using pre-treatment with a mixture of toluene and ethanol allowing to form swelled surface layer followed by gelatin adsorption from its solution in PBS. Our results demonstrate that gelatin immobilization leads to a decrease in the water contact angle from 120° to 0° enhances scaffold strength up to 50%, and doubles the number of adhered cells and their average area. We observed that the maximum amount of gelatin (0.07 ± 0.01 mg per cm3 of the scaffold) was immobilized during the first five minutes of exposure to the gelatin solution. Modified scaffolds demonstrated increased strength.
Original language | English |
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Pages (from-to) | 137-140 |
Number of pages | 4 |
Journal | Colloids and Surfaces B: Biointerfaces |
Volume | 177 |
DOIs | |
Publication status | Published - 1 May 2019 |
Keywords
- Electrospun scaffolds
- Gelatin
- Polylactic acid
- Surface modification
ASJC Scopus subject areas
- Biotechnology
- Surfaces and Interfaces
- Physical and Theoretical Chemistry
- Colloid and Surface Chemistry