“Solvent/non-solvent” treatment as a method for non-covalent immobilization of gelatin on the surface of poly(L-lactic acid) electrospun scaffolds

S. I. Goreninskii, R. O. Guliaev, K. S. Stankevich, N. V. Danilenko, E. N. Bolbasov, A. S. Golovkin, A. I. Mishanin, V. D. Filimonov, S. I. Tverdokhlebov

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In the present study, we report a simple and efficient method of gelatin immobilization on the surface of PLA electrospun fibers using pre-treatment with a mixture of toluene and ethanol allowing to form swelled surface layer followed by gelatin adsorption from its solution in PBS. Our results demonstrate that gelatin immobilization leads to a decrease in the water contact angle from 120° to 0° enhances scaffold strength up to 50%, and doubles the number of adhered cells and their average area. We observed that the maximum amount of gelatin (0.07 ± 0.01 mg per cm3 of the scaffold) was immobilized during the first five minutes of exposure to the gelatin solution. Modified scaffolds demonstrated increased strength.

Original languageEnglish
Pages (from-to)137-140
Number of pages4
JournalColloids and Surfaces B: Biointerfaces
Volume177
DOIs
Publication statusPublished - 1 May 2019

Keywords

  • Electrospun scaffolds
  • Gelatin
  • Polylactic acid
  • Surface modification

ASJC Scopus subject areas

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

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