“Solvent/non-solvent” treatment as a method for non-covalent immobilization of gelatin on the surface of poly(L-lactic acid) electrospun scaffolds

Abstract

In the present study, we report a simple and efficient method of gelatin immobilization on the surface of PLA electrospun fibers using pre-treatment with a mixture of toluene and ethanol allowing to form swelled surface layer followed by gelatin adsorption from its solution in PBS. Our results demonstrate that gelatin immobilization leads to a decrease in the water contact angle from 120° to 0° enhances scaffold strength up to 50%, and doubles the number of adhered cells and their average area. We observed that the maximum amount of gelatin (0.07 ± 0.01 mg per cm³ of the scaffold) was immobilized during the first five minutes of exposure to the gelatin solution. Modified scaffolds demonstrated increased strength.

Original language	English
Pages (from-to)	137-140
Number of pages	4
Journal	Colloids and Surfaces B: Biointerfaces
Volume	177
DOIs	https://doi.org/10.1016/j.colsurfb.2019.01.060
Publication status	Published - 1 May 2019

Keywords

Electrospun scaffolds
Gelatin
Polylactic acid
Surface modification

ASJC Scopus subject areas

Biotechnology
Surfaces and Interfaces
Physical and Theoretical Chemistry
Colloid and Surface Chemistry

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10.1016/j.colsurfb.2019.01.060

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Goreninskii, S. I., Guliaev, R. O., Stankevich, K. S., Danilenko, N. V., Bolbasov, E. N., Golovkin, A. S., Mishanin, A. I., Filimonov, V. D., & Tverdokhlebov, S. I. (2019). “Solvent/non-solvent” treatment as a method for non-covalent immobilization of gelatin on the surface of poly(L-lactic acid) electrospun scaffolds. Colloids and Surfaces B: Biointerfaces, 177, 137-140. https://doi.org/10.1016/j.colsurfb.2019.01.060

“Solvent/non-solvent” treatment as a method for non-covalent immobilization of gelatin on the surface of poly(L-lactic acid) electrospun scaffolds. / Goreninskii, S. I.; Guliaev, R. O.; Stankevich, K. S.; Danilenko, N. V.; Bolbasov, E. N.; Golovkin, A. S.; Mishanin, A. I.; Filimonov, V. D.; Tverdokhlebov, S. I.

In: Colloids and Surfaces B: Biointerfaces, Vol. 177, 01.05.2019, p. 137-140.

Research output: Contribution to journal › Article › peer-review

Goreninskii, SI, Guliaev, RO, Stankevich, KS, Danilenko, NV, Bolbasov, EN, Golovkin, AS, Mishanin, AI, Filimonov, VD & Tverdokhlebov, SI 2019, '“Solvent/non-solvent” treatment as a method for non-covalent immobilization of gelatin on the surface of poly(L-lactic acid) electrospun scaffolds', Colloids and Surfaces B: Biointerfaces, vol. 177, pp. 137-140. https://doi.org/10.1016/j.colsurfb.2019.01.060

Goreninskii, S. I. ; Guliaev, R. O. ; Stankevich, K. S. ; Danilenko, N. V. ; Bolbasov, E. N. ; Golovkin, A. S. ; Mishanin, A. I. ; Filimonov, V. D. ; Tverdokhlebov, S. I. / “Solvent/non-solvent” treatment as a method for non-covalent immobilization of gelatin on the surface of poly(L-lactic acid) electrospun scaffolds. In: Colloids and Surfaces B: Biointerfaces. 2019 ; Vol. 177. pp. 137-140.

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abstract = "In the present study, we report a simple and efficient method of gelatin immobilization on the surface of PLA electrospun fibers using pre-treatment with a mixture of toluene and ethanol allowing to form swelled surface layer followed by gelatin adsorption from its solution in PBS. Our results demonstrate that gelatin immobilization leads to a decrease in the water contact angle from 120° to 0° enhances scaffold strength up to 50%, and doubles the number of adhered cells and their average area. We observed that the maximum amount of gelatin (0.07 ± 0.01 mg per cm3 of the scaffold) was immobilized during the first five minutes of exposure to the gelatin solution. Modified scaffolds demonstrated increased strength.",

keywords = "Electrospun scaffolds, Gelatin, Polylactic acid, Surface modification",

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AU - Goreninskii, S. I.

AU - Guliaev, R. O.

AU - Stankevich, K. S.

AU - Danilenko, N. V.

AU - Bolbasov, E. N.

AU - Golovkin, A. S.

AU - Mishanin, A. I.

AU - Filimonov, V. D.

AU - Tverdokhlebov, S. I.

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AB - In the present study, we report a simple and efficient method of gelatin immobilization on the surface of PLA electrospun fibers using pre-treatment with a mixture of toluene and ethanol allowing to form swelled surface layer followed by gelatin adsorption from its solution in PBS. Our results demonstrate that gelatin immobilization leads to a decrease in the water contact angle from 120° to 0° enhances scaffold strength up to 50%, and doubles the number of adhered cells and their average area. We observed that the maximum amount of gelatin (0.07 ± 0.01 mg per cm3 of the scaffold) was immobilized during the first five minutes of exposure to the gelatin solution. Modified scaffolds demonstrated increased strength.

KW - Electrospun scaffolds

KW - Gelatin

KW - Polylactic acid

KW - Surface modification

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