“Solvent/non-solvent” treatment as a method for non-covalent immobilization of gelatin on the surface of poly(L-lactic acid) electrospun scaffolds

S. I. Goreninskii, R. O. Guliaev, K. S. Stankevich, N. V. Danilenko, E. N. Bolbasov, A. S. Golovkin, A. I. Mishanin, V. D. Filimonov, S. I. Tverdokhlebov

Research output: Contribution to journal › Article

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Abstract

In the present study, we report a simple and efficient method of gelatin immobilization on the surface of PLA electrospun fibers using pre-treatment with a mixture of toluene and ethanol allowing to form swelled surface layer followed by gelatin adsorption from its solution in PBS. Our results demonstrate that gelatin immobilization leads to a decrease in the water contact angle from 120° to 0° enhances scaffold strength up to 50%, and doubles the number of adhered cells and their average area. We observed that the maximum amount of gelatin (0.07 ± 0.01 mg per cm³ of the scaffold) was immobilized during the first five minutes of exposure to the gelatin solution. Modified scaffolds demonstrated increased strength.

Original language	English
Pages (from-to)	137-140
Number of pages	4
Journal	Colloids and Surfaces B: Biointerfaces
Volume	177
DOIs	https://doi.org/10.1016/j.colsurfb.2019.01.060
Publication status	Published - 1 May 2019

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Keywords

Electrospun scaffolds
Gelatin
Polylactic acid
Surface modification

ASJC Scopus subject areas

Biotechnology
Surfaces and Interfaces
Physical and Theoretical Chemistry
Colloid and Surface Chemistry

Cite this

Goreninskii, S. I., Guliaev, R. O., Stankevich, K. S., Danilenko, N. V., Bolbasov, E. N., Golovkin, A. S., Mishanin, A. I., Filimonov, V. D., & Tverdokhlebov, S. I. (2019). “Solvent/non-solvent” treatment as a method for non-covalent immobilization of gelatin on the surface of poly(L-lactic acid) electrospun scaffolds. Colloids and Surfaces B: Biointerfaces, 177, 137-140. https://doi.org/10.1016/j.colsurfb.2019.01.060

“Solvent/non-solvent” treatment as a method for non-covalent immobilization of gelatin on the surface of poly(L-lactic acid) electrospun scaffolds. / Goreninskii, S. I.; Guliaev, R. O.; Stankevich, K. S.; Danilenko, N. V.; Bolbasov, E. N.; Golovkin, A. S.; Mishanin, A. I.; Filimonov, V. D.; Tverdokhlebov, S. I.

In: Colloids and Surfaces B: Biointerfaces, Vol. 177, 01.05.2019, p. 137-140.

Research output: Contribution to journal › Article

Goreninskii, SI, Guliaev, RO, Stankevich, KS, Danilenko, NV, Bolbasov, EN, Golovkin, AS, Mishanin, AI, Filimonov, VD & Tverdokhlebov, SI 2019, '“Solvent/non-solvent” treatment as a method for non-covalent immobilization of gelatin on the surface of poly(L-lactic acid) electrospun scaffolds', Colloids and Surfaces B: Biointerfaces, vol. 177, pp. 137-140. https://doi.org/10.1016/j.colsurfb.2019.01.060

Goreninskii, S. I. ; Guliaev, R. O. ; Stankevich, K. S. ; Danilenko, N. V. ; Bolbasov, E. N. ; Golovkin, A. S. ; Mishanin, A. I. ; Filimonov, V. D. ; Tverdokhlebov, S. I. / “Solvent/non-solvent” treatment as a method for non-covalent immobilization of gelatin on the surface of poly(L-lactic acid) electrospun scaffolds. In: Colloids and Surfaces B: Biointerfaces. 2019 ; Vol. 177. pp. 137-140.

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abstract = "In the present study, we report a simple and efficient method of gelatin immobilization on the surface of PLA electrospun fibers using pre-treatment with a mixture of toluene and ethanol allowing to form swelled surface layer followed by gelatin adsorption from its solution in PBS. Our results demonstrate that gelatin immobilization leads to a decrease in the water contact angle from 120° to 0° enhances scaffold strength up to 50%, and doubles the number of adhered cells and their average area. We observed that the maximum amount of gelatin (0.07 ± 0.01 mg per cm3 of the scaffold) was immobilized during the first five minutes of exposure to the gelatin solution. Modified scaffolds demonstrated increased strength.",

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AU - Stankevich, K. S.

AU - Danilenko, N. V.

AU - Bolbasov, E. N.

AU - Golovkin, A. S.

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AB - In the present study, we report a simple and efficient method of gelatin immobilization on the surface of PLA electrospun fibers using pre-treatment with a mixture of toluene and ethanol allowing to form swelled surface layer followed by gelatin adsorption from its solution in PBS. Our results demonstrate that gelatin immobilization leads to a decrease in the water contact angle from 120° to 0° enhances scaffold strength up to 50%, and doubles the number of adhered cells and their average area. We observed that the maximum amount of gelatin (0.07 ± 0.01 mg per cm3 of the scaffold) was immobilized during the first five minutes of exposure to the gelatin solution. Modified scaffolds demonstrated increased strength.

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10.1016/j.colsurfb.2019.01.060