Abstract
In the present study, we report a simple and efficient method of gelatin immobilization on the surface of PLA electrospun fibers using pre-treatment with a mixture of toluene and ethanol allowing to form swelled surface layer followed by gelatin adsorption from its solution in PBS. Our results demonstrate that gelatin immobilization leads to a decrease in the water contact angle from 120° to 0° enhances scaffold strength up to 50%, and doubles the number of adhered cells and their average area. We observed that the maximum amount of gelatin (0.07 ± 0.01 mg per cm3 of the scaffold) was immobilized during the first five minutes of exposure to the gelatin solution. Modified scaffolds demonstrated increased strength.
| Original language | English |
|---|---|
| Pages (from-to) | 137-140 |
| Number of pages | 4 |
| Journal | Colloids and Surfaces B: Biointerfaces |
| Volume | 177 |
| DOIs | |
| Publication status | Published - 1 May 2019 |
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Keywords
- Electrospun scaffolds
- Gelatin
- Polylactic acid
- Surface modification
ASJC Scopus subject areas
- Biotechnology
- Surfaces and Interfaces
- Physical and Theoretical Chemistry
- Colloid and Surface Chemistry
Cite this
“Solvent/non-solvent” treatment as a method for non-covalent immobilization of gelatin on the surface of poly(L-lactic acid) electrospun scaffolds. / Goreninskii, S. I.; Guliaev, R. O.; Stankevich, K. S.; Danilenko, N. V.; Bolbasov, E. N.; Golovkin, A. S.; Mishanin, A. I.; Filimonov, V. D.; Tverdokhlebov, S. I.
In: Colloids and Surfaces B: Biointerfaces, Vol. 177, 01.05.2019, p. 137-140.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - “Solvent/non-solvent” treatment as a method for non-covalent immobilization of gelatin on the surface of poly(L-lactic acid) electrospun scaffolds
AU - Goreninskii, S. I.
AU - Guliaev, R. O.
AU - Stankevich, K. S.
AU - Danilenko, N. V.
AU - Bolbasov, E. N.
AU - Golovkin, A. S.
AU - Mishanin, A. I.
AU - Filimonov, V. D.
AU - Tverdokhlebov, S. I.
PY - 2019/5/1
Y1 - 2019/5/1
N2 - In the present study, we report a simple and efficient method of gelatin immobilization on the surface of PLA electrospun fibers using pre-treatment with a mixture of toluene and ethanol allowing to form swelled surface layer followed by gelatin adsorption from its solution in PBS. Our results demonstrate that gelatin immobilization leads to a decrease in the water contact angle from 120° to 0° enhances scaffold strength up to 50%, and doubles the number of adhered cells and their average area. We observed that the maximum amount of gelatin (0.07 ± 0.01 mg per cm3 of the scaffold) was immobilized during the first five minutes of exposure to the gelatin solution. Modified scaffolds demonstrated increased strength.
AB - In the present study, we report a simple and efficient method of gelatin immobilization on the surface of PLA electrospun fibers using pre-treatment with a mixture of toluene and ethanol allowing to form swelled surface layer followed by gelatin adsorption from its solution in PBS. Our results demonstrate that gelatin immobilization leads to a decrease in the water contact angle from 120° to 0° enhances scaffold strength up to 50%, and doubles the number of adhered cells and their average area. We observed that the maximum amount of gelatin (0.07 ± 0.01 mg per cm3 of the scaffold) was immobilized during the first five minutes of exposure to the gelatin solution. Modified scaffolds demonstrated increased strength.
KW - Electrospun scaffolds
KW - Gelatin
KW - Polylactic acid
KW - Surface modification
UR - http://www.scopus.com/inward/record.url?scp=85060895046&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85060895046&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfb.2019.01.060
DO - 10.1016/j.colsurfb.2019.01.060
M3 - Article
VL - 177
SP - 137
EP - 140
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
SN - 0927-7765
ER -