Simultaneous Determination of Succinic Acid and Water-Soluble Vitamins by Ion-Pair High-Performance Liquid Chromatography

K. A. Leonov, A. V. Pustovoitov, D. A. Vishenkova

Research output: Contribution to journalArticle

Abstract

A procedure is proposed for the determination of succinic acid, riboxin, nicotinamide, and riboflavin by ion-pair HPLC with UV detection. Owing to the addition of an ion-pair modifier to the mobile phase and the selection of gradient elution conditions, the optimal retention and resolution of peaks of the components to be determined are achieved. Specificity, linearity, accuracy, and precision of the developed procedure are proved on an example of the determination of active substances in the Cerebronorm® preparation.

Original languageEnglish
Pages (from-to)358-363
Number of pages6
JournalJournal of Analytical Chemistry
Volume73
Issue number4
DOIs
Publication statusPublished - 1 Apr 2018

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Keywords

  • Cerebronorm®
  • gradient elution
  • ion-pair HPLC
  • succinic acid
  • water-soluble vitamins

ASJC Scopus subject areas

  • Analytical Chemistry

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