Simultaneous Determination of Succinic Acid and Water-Soluble Vitamins by Ion-Pair High-Performance Liquid Chromatography

Abstract

A procedure is proposed for the determination of succinic acid, riboxin, nicotinamide, and riboflavin by ion-pair HPLC with UV detection. Owing to the addition of an ion-pair modifier to the mobile phase and the selection of gradient elution conditions, the optimal retention and resolution of peaks of the components to be determined are achieved. Specificity, linearity, accuracy, and precision of the developed procedure are proved on an example of the determination of active substances in the Cerebronorm® preparation.

Original language	English
Pages (from-to)	358-363
Number of pages	6
Journal	Journal of Analytical Chemistry
Volume	73
Issue number	4
DOIs	https://doi.org/10.1134/S1061934818040068
Publication status	Published - 1 Apr 2018

Keywords

Cerebronorm®
gradient elution
ion-pair HPLC
succinic acid
water-soluble vitamins

ASJC Scopus subject areas

Analytical Chemistry

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10.1134/S1061934818040068

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@article{e1e821b1c81b4590bf4f0487fca5b7fa,

title = "Simultaneous Determination of Succinic Acid and Water-Soluble Vitamins by Ion-Pair High-Performance Liquid Chromatography",

abstract = "A procedure is proposed for the determination of succinic acid, riboxin, nicotinamide, and riboflavin by ion-pair HPLC with UV detection. Owing to the addition of an ion-pair modifier to the mobile phase and the selection of gradient elution conditions, the optimal retention and resolution of peaks of the components to be determined are achieved. Specificity, linearity, accuracy, and precision of the developed procedure are proved on an example of the determination of active substances in the Cerebronorm{\textregistered} preparation.",

keywords = "Cerebronorm{\textregistered}, gradient elution, ion-pair HPLC, succinic acid, water-soluble vitamins",

author = "Leonov, {K. A.} and Pustovoitov, {A. V.} and Vishenkova, {D. A.}",

year = "2018",

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day = "1",

doi = "10.1134/S1061934818040068",

language = "English",

volume = "73",

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journal = "Journal of Analytical Chemistry",

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publisher = "Maik Nauka-Interperiodica Publishing",

number = "4",

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TY - JOUR

T1 - Simultaneous Determination of Succinic Acid and Water-Soluble Vitamins by Ion-Pair High-Performance Liquid Chromatography

AU - Leonov, K. A.

AU - Pustovoitov, A. V.

AU - Vishenkova, D. A.

PY - 2018/4/1

Y1 - 2018/4/1

N2 - A procedure is proposed for the determination of succinic acid, riboxin, nicotinamide, and riboflavin by ion-pair HPLC with UV detection. Owing to the addition of an ion-pair modifier to the mobile phase and the selection of gradient elution conditions, the optimal retention and resolution of peaks of the components to be determined are achieved. Specificity, linearity, accuracy, and precision of the developed procedure are proved on an example of the determination of active substances in the Cerebronorm® preparation.

AB - A procedure is proposed for the determination of succinic acid, riboxin, nicotinamide, and riboflavin by ion-pair HPLC with UV detection. Owing to the addition of an ion-pair modifier to the mobile phase and the selection of gradient elution conditions, the optimal retention and resolution of peaks of the components to be determined are achieved. Specificity, linearity, accuracy, and precision of the developed procedure are proved on an example of the determination of active substances in the Cerebronorm® preparation.

KW - Cerebronorm®

KW - gradient elution

KW - ion-pair HPLC

KW - succinic acid

KW - water-soluble vitamins

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U2 - 10.1134/S1061934818040068

DO - 10.1134/S1061934818040068

M3 - Article

AN - SCOPUS:85045760061

VL - 73

SP - 358

EP - 363

JO - Journal of Analytical Chemistry

JF - Journal of Analytical Chemistry

SN - 1061-9348

IS - 4