Abstract
Homovanilic (HVA) and vanillylmandelic (VMA) acid are catecholamines metabolites commonly used as cancer biomarkers. New method for simultaneous determination of both these metabolites is presented, using recently developed flow-through coulometric detector based on glassy carbon microbeads. This application presents the potential opportunities of the above mentioned coulometric detector for the simultaneous determination of mixture of substances in complicated matrix in combination with gradient chromatography. Optimum conditions were set as follows: mobile phase consisting of acetonitrile (eluent A) and ten time diluted B-R buffer pH 3 (eluent B) with gradient program 5 % A (2 min), 5 % A to 25 % A (8 min), 25 % A (4 min), 25 % A to 95 % A (1 min), and 95 % A (4 min); flow rate of mobile phase 0.7 mL min−1, detection potential +1.3 V, and injection volume 20 μL. Calibration dependences of both metabolites were linear from concentration 100 μmol L−1 to their quantitation limits (i.e. 0.6 μmol L−1 for HVA and 1.3 μmol L−1 for VMA, respectively). Standard addition method was applied for the determination of these analytes in human urine. Obtained results are in agreement with results of HPLC method with UV detector.
| Original language | English |
|---|---|
| Pages (from-to) | 1455-1460 |
| Number of pages | 6 |
| Journal | Electroanalysis |
| Volume | 30 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 1 Jul 2018 |
| Externally published | Yes |
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Keywords
- Coulometric detection
- Glassy carbon microbeads
- HPLC
- Tumor markers
ASJC Scopus subject areas
- Analytical Chemistry
- Electrochemistry
Cite this
Simultaneous Determination of Homovanillic and Vanillylmandelic Acid by HPLC Using a Coulometric Detector with Renewable Glassy Carbon Microbeads Based Working Electrode. / Mika, Jan; Barek, Jiří; Zima, Jiří; Moreira, Josino Costa; Dejmková, Hana.
In: Electroanalysis, Vol. 30, No. 7, 01.07.2018, p. 1455-1460.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Simultaneous Determination of Homovanillic and Vanillylmandelic Acid by HPLC Using a Coulometric Detector with Renewable Glassy Carbon Microbeads Based Working Electrode
AU - Mika, Jan
AU - Barek, Jiří
AU - Zima, Jiří
AU - Moreira, Josino Costa
AU - Dejmková, Hana
PY - 2018/7/1
Y1 - 2018/7/1
N2 - Homovanilic (HVA) and vanillylmandelic (VMA) acid are catecholamines metabolites commonly used as cancer biomarkers. New method for simultaneous determination of both these metabolites is presented, using recently developed flow-through coulometric detector based on glassy carbon microbeads. This application presents the potential opportunities of the above mentioned coulometric detector for the simultaneous determination of mixture of substances in complicated matrix in combination with gradient chromatography. Optimum conditions were set as follows: mobile phase consisting of acetonitrile (eluent A) and ten time diluted B-R buffer pH 3 (eluent B) with gradient program 5 % A (2 min), 5 % A to 25 % A (8 min), 25 % A (4 min), 25 % A to 95 % A (1 min), and 95 % A (4 min); flow rate of mobile phase 0.7 mL min−1, detection potential +1.3 V, and injection volume 20 μL. Calibration dependences of both metabolites were linear from concentration 100 μmol L−1 to their quantitation limits (i.e. 0.6 μmol L−1 for HVA and 1.3 μmol L−1 for VMA, respectively). Standard addition method was applied for the determination of these analytes in human urine. Obtained results are in agreement with results of HPLC method with UV detector.
AB - Homovanilic (HVA) and vanillylmandelic (VMA) acid are catecholamines metabolites commonly used as cancer biomarkers. New method for simultaneous determination of both these metabolites is presented, using recently developed flow-through coulometric detector based on glassy carbon microbeads. This application presents the potential opportunities of the above mentioned coulometric detector for the simultaneous determination of mixture of substances in complicated matrix in combination with gradient chromatography. Optimum conditions were set as follows: mobile phase consisting of acetonitrile (eluent A) and ten time diluted B-R buffer pH 3 (eluent B) with gradient program 5 % A (2 min), 5 % A to 25 % A (8 min), 25 % A (4 min), 25 % A to 95 % A (1 min), and 95 % A (4 min); flow rate of mobile phase 0.7 mL min−1, detection potential +1.3 V, and injection volume 20 μL. Calibration dependences of both metabolites were linear from concentration 100 μmol L−1 to their quantitation limits (i.e. 0.6 μmol L−1 for HVA and 1.3 μmol L−1 for VMA, respectively). Standard addition method was applied for the determination of these analytes in human urine. Obtained results are in agreement with results of HPLC method with UV detector.
KW - Coulometric detection
KW - Glassy carbon microbeads
KW - HPLC
KW - Tumor markers
UR - http://www.scopus.com/inward/record.url?scp=85045103194&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85045103194&partnerID=8YFLogxK
U2 - 10.1002/elan.201800041
DO - 10.1002/elan.201800041
M3 - Article
VL - 30
SP - 1455
EP - 1460
JO - Macromolecular Chemistry and Physics
JF - Macromolecular Chemistry and Physics
SN - 1040-0397
IS - 7
ER -