Signaling events during macrophage activation with betula pendula roth pectic polysaccharides

A. A. Ligacheva, M. G. Danilets, E. S. Trofimova, Y. P. Belsky, N. V. Belska, G. N. Zyuz'Kov, V. V. Zhdanov, A. N. Ivanova, A. M. Guriev, M. V. Belousov, M. S. Yusubov, A. M. Dygai

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


We studied the effect of two pectic polysaccharides PS-B1-AG and PS-B2-RG that were contained in total polysaccharides extracted from Betula pendula leaves on NO production by mouse macrophages and the contribution of signaling molecules to macrophage activation by the test substances. Unlike the total sample, pectins produced a NO-stimulating effect on macrophages. The effect of PS-B2-RG (10 μg/ml) did not differ from the effect of LPS, while PS-B1-AG produced this effect only in a concentration of 20 μg/ml, which was probably due to differences in the chemical structure of the test substances. The studied pectin polysaccharides activated transcription factor NF-κB, kinases p38 and PI3, and cAMP as a negative regulator. These results indicate that Betula pendula polysaccharides are promising substances for creation of immunomodulating drugs.

Original languageEnglish
Pages (from-to)465-469
Number of pages5
JournalBulletin of Experimental Biology and Medicine
Issue number4
Publication statusPublished - 1 Jan 2014


  • immune response
  • macrophages
  • nitric oxide
  • plant polysaccharides
  • signaling molecules

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Signaling events during macrophage activation with betula pendula roth pectic polysaccharides'. Together they form a unique fingerprint.

Cite this