Self-Assembling Complexes of Quantum Dots and scFv Antibodies for Cancer Cell Targeting and Imaging

Tatiana A. Zdobnova, Oleg A. Stremovskiy, Ekaterina N. Lebedenko, Sergey M. Deyev

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Semiconductor quantum dots represent a novel class of fluorophores with unique physical and chemical properties which could enable a remarkable broadening of the current applications of fluorescent imaging and optical diagnostics. Complexes of quantum dots and antibodies are promising visualising agents for fluorescent detection of selective biomarkers overexpressed in tumor tissues. Here we describe the construction of self-assembling fluorescent complexes of quantum dots and anti-HER1 or anti-HER2/neu scFv antibodies and their interactions with cultured tumor cells. A binding strategy based on a very specific non-covalent interaction between two proteins, barnase and barstar, was used to connect quantum dots and the targeting antibodies. Such a strategy allows combining the targeting and visualization functions simply by varying the corresponding modules of the fluorescent complex.

Original languageEnglish
Article numbere48248
JournalPLoS One
Volume7
Issue number10
DOIs
Publication statusPublished - 25 Oct 2012
Externally publishedYes

Fingerprint

quantum dots
Single-Chain Antibodies
Quantum Dots
Semiconductor quantum dots
Cells
image analysis
Imaging techniques
antibodies
Tumors
Neoplasms
Cultured Tumor Cells
Semiconductors
Fluorophores
Antibodies
fluorescent dyes
Optical Imaging
Biomarkers
Diagnostic Imaging
Fluorescent Dyes
Chemical properties

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Self-Assembling Complexes of Quantum Dots and scFv Antibodies for Cancer Cell Targeting and Imaging. / Zdobnova, Tatiana A.; Stremovskiy, Oleg A.; Lebedenko, Ekaterina N.; Deyev, Sergey M.

In: PLoS One, Vol. 7, No. 10, e48248, 25.10.2012.

Research output: Contribution to journalArticle

Zdobnova, Tatiana A. ; Stremovskiy, Oleg A. ; Lebedenko, Ekaterina N. ; Deyev, Sergey M. / Self-Assembling Complexes of Quantum Dots and scFv Antibodies for Cancer Cell Targeting and Imaging. In: PLoS One. 2012 ; Vol. 7, No. 10.
@article{3f4915a4c36d436ab02341564dcc6449,
title = "Self-Assembling Complexes of Quantum Dots and scFv Antibodies for Cancer Cell Targeting and Imaging",
abstract = "Semiconductor quantum dots represent a novel class of fluorophores with unique physical and chemical properties which could enable a remarkable broadening of the current applications of fluorescent imaging and optical diagnostics. Complexes of quantum dots and antibodies are promising visualising agents for fluorescent detection of selective biomarkers overexpressed in tumor tissues. Here we describe the construction of self-assembling fluorescent complexes of quantum dots and anti-HER1 or anti-HER2/neu scFv antibodies and their interactions with cultured tumor cells. A binding strategy based on a very specific non-covalent interaction between two proteins, barnase and barstar, was used to connect quantum dots and the targeting antibodies. Such a strategy allows combining the targeting and visualization functions simply by varying the corresponding modules of the fluorescent complex.",
author = "Zdobnova, {Tatiana A.} and Stremovskiy, {Oleg A.} and Lebedenko, {Ekaterina N.} and Deyev, {Sergey M.}",
year = "2012",
month = "10",
day = "25",
doi = "10.1371/journal.pone.0048248",
language = "English",
volume = "7",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "10",

}

TY - JOUR

T1 - Self-Assembling Complexes of Quantum Dots and scFv Antibodies for Cancer Cell Targeting and Imaging

AU - Zdobnova, Tatiana A.

AU - Stremovskiy, Oleg A.

AU - Lebedenko, Ekaterina N.

AU - Deyev, Sergey M.

PY - 2012/10/25

Y1 - 2012/10/25

N2 - Semiconductor quantum dots represent a novel class of fluorophores with unique physical and chemical properties which could enable a remarkable broadening of the current applications of fluorescent imaging and optical diagnostics. Complexes of quantum dots and antibodies are promising visualising agents for fluorescent detection of selective biomarkers overexpressed in tumor tissues. Here we describe the construction of self-assembling fluorescent complexes of quantum dots and anti-HER1 or anti-HER2/neu scFv antibodies and their interactions with cultured tumor cells. A binding strategy based on a very specific non-covalent interaction between two proteins, barnase and barstar, was used to connect quantum dots and the targeting antibodies. Such a strategy allows combining the targeting and visualization functions simply by varying the corresponding modules of the fluorescent complex.

AB - Semiconductor quantum dots represent a novel class of fluorophores with unique physical and chemical properties which could enable a remarkable broadening of the current applications of fluorescent imaging and optical diagnostics. Complexes of quantum dots and antibodies are promising visualising agents for fluorescent detection of selective biomarkers overexpressed in tumor tissues. Here we describe the construction of self-assembling fluorescent complexes of quantum dots and anti-HER1 or anti-HER2/neu scFv antibodies and their interactions with cultured tumor cells. A binding strategy based on a very specific non-covalent interaction between two proteins, barnase and barstar, was used to connect quantum dots and the targeting antibodies. Such a strategy allows combining the targeting and visualization functions simply by varying the corresponding modules of the fluorescent complex.

UR - http://www.scopus.com/inward/record.url?scp=84868108817&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84868108817&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0048248

DO - 10.1371/journal.pone.0048248

M3 - Article

VL - 7

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 10

M1 - e48248

ER -