сеКРеЦиЯ сигналЬнЫХ МолеКУл КРоветвоРнЫХ ниШ в УсловиЯХ остеогенноЙ диФФеРенЦиРовКи МУлЬтиПотентнЫХ МеЗенХиМнЫХ стРоМалЬнЫХ КлетоК, индУЦиРованноЙ РелЬеФнЫМ КалЬЦиЙ-ФосФатнЫМ ПоКРЫтиеМ

Translated title of the contribution: Secretion of niche signal molecules in conditions of osteogenic differentiation of multipotent mesenchymal stromal cells induced by textured calcium phosphate coating

L. S. Litvinova, V. V. Shupletsova, K. A. Yurova, O. G. Khaziakhmatova, N. M. Todosenko, V. V. Malashchenko, E. O. Shunkin, E. S. Melashchenko, M. Yu Khlusova, E. G. Komarova, V. V. Chebodaeva, Yu P. Sharkeev, P. A. Ivanov, I. A. Khlusov

Research output: Contribution to journalArticle

Abstract

Secretion of 21 cytokines, chemokines and growth factors (LIF, SCF, SDF-1a, SCGF-b, M-CSF, MCP-3, MIF, MIG, TRAIL, GRO-a; IL-1a, IL-2ra, IL-3, IL-12(p40), IL-16, IL-18, HGF, TNF-b, b-NGF, IFN-a2, CTACK) has been studied in vitro in the culture of human adipose-derived multipotent mesenchymal stromal cells (hAMMSCs) in conditions of its osteogenic differentiation caused by 14-day contact with calcium phosphate (CP) surface with different roughness. Bilateral X-ray amorphous CP coatings were prepared on the samples of commercially pure titanium in the anodal regime using a micro-arc method. An aqueous solution prepared from 20 wt% phosphoric acid, 6 wt% dissolved hydrohyapatite nanopowder (particle diameter 10-30 nm with single agglomerates up to 100 nm), and 9 wt% dissolved calcium carbonate was used to obtain CP coating. hAMMSCs isolated from lipoaspirate were co-cultured after 4 passages with the CP-coated samples at final concentration of 1.5´105 viable karyocytes per 1.5 mL of standard nutrition medium (without osteogenic stimulators) for 14 days (a determination of [CD45,34,14,20], CD73, CD90 и CD105 cell immunophenotype; an analysis of secretory activity) and 21 days (alizarin red S staining of culture) with medium replacement every 3-4 days. Under conditions of in vitro contact with rough CP coating hAMMSCs differentiated into osteoblasts synthesizing the mineralized bone matrix; this was accompanied by 2-3-fold increasing ratio of [CD45,34,14,20]+ hemopoietic cells. The following humoral factors of hemopoietic niches acted as the signal molecules escalating in vitro the hemopoietic base in 14 days of differentiating three-dimensional culture of hAMMSCs: either leukemia inhibitory factor (LIF) and stem cell factor (SCF) cytokines under mean index of CP roughness Ra=2.4-2.6 mm or stromal derived factor-1 (SDF-1a, CXCL12 chemokine) under Ra=3.1-4.4 mm.

Original languageRussian
Pages (from-to)339-346
Number of pages8
JournalBiomeditsinskaya Khimiya
Volume65
Issue number4
DOIs
Publication statusPublished - 1 Jan 2019

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Mesenchymal Stromal Cells
Cells
Coatings
Molecules
Leukemia Inhibitory Factor
Stem Cell Factor
Interleukin-16
Surface roughness
Cytokines
Phosphate coatings
Chemokine CXCL12
Bone Matrix
Interleukin-18
Macrophage Colony-Stimulating Factor
Calcium Carbonate
Interleukin-3
Osteoblasts
Nerve Growth Factor
Interleukin-12
Nutrition

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

сеКРеЦиЯ сигналЬнЫХ МолеКУл КРоветвоРнЫХ ниШ в УсловиЯХ остеогенноЙ диФФеРенЦиРовКи МУлЬтиПотентнЫХ МеЗенХиМнЫХ стРоМалЬнЫХ КлетоК, индУЦиРованноЙ РелЬеФнЫМ КалЬЦиЙ-ФосФатнЫМ ПоКРЫтиеМ. / Litvinova, L. S.; Shupletsova, V. V.; Yurova, K. A.; Khaziakhmatova, O. G.; Todosenko, N. M.; Malashchenko, V. V.; Shunkin, E. O.; Melashchenko, E. S.; Khlusova, M. Yu; Komarova, E. G.; Chebodaeva, V. V.; Sharkeev, Yu P.; Ivanov, P. A.; Khlusov, I. A.

In: Biomeditsinskaya Khimiya, Vol. 65, No. 4, 01.01.2019, p. 339-346.

Research output: Contribution to journalArticle

Litvinova, L. S. ; Shupletsova, V. V. ; Yurova, K. A. ; Khaziakhmatova, O. G. ; Todosenko, N. M. ; Malashchenko, V. V. ; Shunkin, E. O. ; Melashchenko, E. S. ; Khlusova, M. Yu ; Komarova, E. G. ; Chebodaeva, V. V. ; Sharkeev, Yu P. ; Ivanov, P. A. ; Khlusov, I. A. / сеКРеЦиЯ сигналЬнЫХ МолеКУл КРоветвоРнЫХ ниШ в УсловиЯХ остеогенноЙ диФФеРенЦиРовКи МУлЬтиПотентнЫХ МеЗенХиМнЫХ стРоМалЬнЫХ КлетоК, индУЦиРованноЙ РелЬеФнЫМ КалЬЦиЙ-ФосФатнЫМ ПоКРЫтиеМ. In: Biomeditsinskaya Khimiya. 2019 ; Vol. 65, No. 4. pp. 339-346.
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author = "Litvinova, {L. S.} and Shupletsova, {V. V.} and Yurova, {K. A.} and Khaziakhmatova, {O. G.} and Todosenko, {N. M.} and Malashchenko, {V. V.} and Shunkin, {E. O.} and Melashchenko, {E. S.} and Khlusova, {M. Yu} and Komarova, {E. G.} and Chebodaeva, {V. V.} and Sharkeev, {Yu P.} and Ivanov, {P. A.} and Khlusov, {I. A.}",
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TY - JOUR

T1 - сеКРеЦиЯ сигналЬнЫХ МолеКУл КРоветвоРнЫХ ниШ в УсловиЯХ остеогенноЙ диФФеРенЦиРовКи МУлЬтиПотентнЫХ МеЗенХиМнЫХ стРоМалЬнЫХ КлетоК, индУЦиРованноЙ РелЬеФнЫМ КалЬЦиЙ-ФосФатнЫМ ПоКРЫтиеМ

AU - Litvinova, L. S.

AU - Shupletsova, V. V.

AU - Yurova, K. A.

AU - Khaziakhmatova, O. G.

AU - Todosenko, N. M.

AU - Malashchenko, V. V.

AU - Shunkin, E. O.

AU - Melashchenko, E. S.

AU - Khlusova, M. Yu

AU - Komarova, E. G.

AU - Chebodaeva, V. V.

AU - Sharkeev, Yu P.

AU - Ivanov, P. A.

AU - Khlusov, I. A.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Secretion of 21 cytokines, chemokines and growth factors (LIF, SCF, SDF-1a, SCGF-b, M-CSF, MCP-3, MIF, MIG, TRAIL, GRO-a; IL-1a, IL-2ra, IL-3, IL-12(p40), IL-16, IL-18, HGF, TNF-b, b-NGF, IFN-a2, CTACK) has been studied in vitro in the culture of human adipose-derived multipotent mesenchymal stromal cells (hAMMSCs) in conditions of its osteogenic differentiation caused by 14-day contact with calcium phosphate (CP) surface with different roughness. Bilateral X-ray amorphous CP coatings were prepared on the samples of commercially pure titanium in the anodal regime using a micro-arc method. An aqueous solution prepared from 20 wt% phosphoric acid, 6 wt% dissolved hydrohyapatite nanopowder (particle diameter 10-30 nm with single agglomerates up to 100 nm), and 9 wt% dissolved calcium carbonate was used to obtain CP coating. hAMMSCs isolated from lipoaspirate were co-cultured after 4 passages with the CP-coated samples at final concentration of 1.5´105 viable karyocytes per 1.5 mL of standard nutrition medium (without osteogenic stimulators) for 14 days (a determination of [CD45,34,14,20], CD73, CD90 и CD105 cell immunophenotype; an analysis of secretory activity) and 21 days (alizarin red S staining of culture) with medium replacement every 3-4 days. Under conditions of in vitro contact with rough CP coating hAMMSCs differentiated into osteoblasts synthesizing the mineralized bone matrix; this was accompanied by 2-3-fold increasing ratio of [CD45,34,14,20]+ hemopoietic cells. The following humoral factors of hemopoietic niches acted as the signal molecules escalating in vitro the hemopoietic base in 14 days of differentiating three-dimensional culture of hAMMSCs: either leukemia inhibitory factor (LIF) and stem cell factor (SCF) cytokines under mean index of CP roughness Ra=2.4-2.6 mm or stromal derived factor-1 (SDF-1a, CXCL12 chemokine) under Ra=3.1-4.4 mm.

AB - Secretion of 21 cytokines, chemokines and growth factors (LIF, SCF, SDF-1a, SCGF-b, M-CSF, MCP-3, MIF, MIG, TRAIL, GRO-a; IL-1a, IL-2ra, IL-3, IL-12(p40), IL-16, IL-18, HGF, TNF-b, b-NGF, IFN-a2, CTACK) has been studied in vitro in the culture of human adipose-derived multipotent mesenchymal stromal cells (hAMMSCs) in conditions of its osteogenic differentiation caused by 14-day contact with calcium phosphate (CP) surface with different roughness. Bilateral X-ray amorphous CP coatings were prepared on the samples of commercially pure titanium in the anodal regime using a micro-arc method. An aqueous solution prepared from 20 wt% phosphoric acid, 6 wt% dissolved hydrohyapatite nanopowder (particle diameter 10-30 nm with single agglomerates up to 100 nm), and 9 wt% dissolved calcium carbonate was used to obtain CP coating. hAMMSCs isolated from lipoaspirate were co-cultured after 4 passages with the CP-coated samples at final concentration of 1.5´105 viable karyocytes per 1.5 mL of standard nutrition medium (without osteogenic stimulators) for 14 days (a determination of [CD45,34,14,20], CD73, CD90 и CD105 cell immunophenotype; an analysis of secretory activity) and 21 days (alizarin red S staining of culture) with medium replacement every 3-4 days. Under conditions of in vitro contact with rough CP coating hAMMSCs differentiated into osteoblasts synthesizing the mineralized bone matrix; this was accompanied by 2-3-fold increasing ratio of [CD45,34,14,20]+ hemopoietic cells. The following humoral factors of hemopoietic niches acted as the signal molecules escalating in vitro the hemopoietic base in 14 days of differentiating three-dimensional culture of hAMMSCs: either leukemia inhibitory factor (LIF) and stem cell factor (SCF) cytokines under mean index of CP roughness Ra=2.4-2.6 mm or stromal derived factor-1 (SDF-1a, CXCL12 chemokine) under Ra=3.1-4.4 mm.

KW - In vitro

KW - Micro-arc coating

KW - Roughness index R

KW - Stem cell factor

KW - Stem cell growth factor

KW - Stromal derived factor-1

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