Abstract
The in vitro effect of sanazole (AK-2123; N-(2′-methoxyethyl)-2- [3″-nitro-1″-triazolyl]acetamide) and metronidazole (1-β-hydroxyethyl-2-methyl-5-nitroimidazole) on phorbol-12-myristate-13- acetate (PMA)-stimulated and spontaneous (without stimulation by PMA) production of reactive oxygen species (ROS) by peritoneal and splenic murine macrophages was studied. ROS production was analyzed using fluorescent probe 2′,7′-dichlorofluoresceine diacetate (DCFH-DA). An increase in the spontaneous production of ROS by macrophagal cells with therapeutic concentration of sanazole (0,6-1,25 mM) in the incubation medium was observed. At these concentrations metronidazole had no effect on spontaneous production of ROS by macrophagal cells. PMA-stimulated ROS production was inhibited by high concentrations (2,5-10 mM) of sanazole and metronidazole. The spontaneous generation of ROS by peritoneal macrophages was stimulated by sanazole at all tested concentrations (0,6-10 mM).
| Original language | English |
|---|---|
| Pages (from-to) | 7-10 |
| Number of pages | 4 |
| Journal | Antibiotiki i Khimioterapiya |
| Volume | 48 |
| Issue number | 3 |
| Publication status | Published - 2003 |
| Externally published | Yes |
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Keywords
- Macrophages
- Metronidazole
- Nitroazoles
- Reactive oxygen species
- Sanazole
ASJC Scopus subject areas
- Microbiology
- Pharmacology
Cite this
Sanazole and metronidazole effect on reactive oxygen species production by macrophages. / Shchepetkin, I. A.; Cherdyntseva, N. V.; Kagiya, V. T.
In: Antibiotiki i Khimioterapiya, Vol. 48, No. 3, 2003, p. 7-10.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Sanazole and metronidazole effect on reactive oxygen species production by macrophages
AU - Shchepetkin, I. A.
AU - Cherdyntseva, N. V.
AU - Kagiya, V. T.
PY - 2003
Y1 - 2003
N2 - The in vitro effect of sanazole (AK-2123; N-(2′-methoxyethyl)-2- [3″-nitro-1″-triazolyl]acetamide) and metronidazole (1-β-hydroxyethyl-2-methyl-5-nitroimidazole) on phorbol-12-myristate-13- acetate (PMA)-stimulated and spontaneous (without stimulation by PMA) production of reactive oxygen species (ROS) by peritoneal and splenic murine macrophages was studied. ROS production was analyzed using fluorescent probe 2′,7′-dichlorofluoresceine diacetate (DCFH-DA). An increase in the spontaneous production of ROS by macrophagal cells with therapeutic concentration of sanazole (0,6-1,25 mM) in the incubation medium was observed. At these concentrations metronidazole had no effect on spontaneous production of ROS by macrophagal cells. PMA-stimulated ROS production was inhibited by high concentrations (2,5-10 mM) of sanazole and metronidazole. The spontaneous generation of ROS by peritoneal macrophages was stimulated by sanazole at all tested concentrations (0,6-10 mM).
AB - The in vitro effect of sanazole (AK-2123; N-(2′-methoxyethyl)-2- [3″-nitro-1″-triazolyl]acetamide) and metronidazole (1-β-hydroxyethyl-2-methyl-5-nitroimidazole) on phorbol-12-myristate-13- acetate (PMA)-stimulated and spontaneous (without stimulation by PMA) production of reactive oxygen species (ROS) by peritoneal and splenic murine macrophages was studied. ROS production was analyzed using fluorescent probe 2′,7′-dichlorofluoresceine diacetate (DCFH-DA). An increase in the spontaneous production of ROS by macrophagal cells with therapeutic concentration of sanazole (0,6-1,25 mM) in the incubation medium was observed. At these concentrations metronidazole had no effect on spontaneous production of ROS by macrophagal cells. PMA-stimulated ROS production was inhibited by high concentrations (2,5-10 mM) of sanazole and metronidazole. The spontaneous generation of ROS by peritoneal macrophages was stimulated by sanazole at all tested concentrations (0,6-10 mM).
KW - Macrophages
KW - Metronidazole
KW - Nitroazoles
KW - Reactive oxygen species
KW - Sanazole
UR - http://www.scopus.com/inward/record.url?scp=0141499071&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0141499071&partnerID=8YFLogxK
M3 - Article
VL - 48
SP - 7
EP - 10
JO - Antibiotiki i Khimioterapiya
JF - Antibiotiki i Khimioterapiya
SN - 0235-2990
IS - 3
ER -