Sanazole and metronidazole effect on reactive oxygen species production by macrophages

The in vitro effect of sanazole (AK-2123; N-(2′-methoxyethyl)-2- [3″-nitro-1″-triazolyl]acetamide) and metronidazole (1-β-hydroxyethyl-2-methyl-5-nitroimidazole) on phorbol-12-myristate-13- acetate (PMA)-stimulated and spontaneous (without stimulation by PMA) production of reactive oxygen species (ROS) by peritoneal and splenic murine macrophages was studied. ROS production was analyzed using fluorescent probe 2′,7′-dichlorofluoresceine diacetate (DCFH-DA). An increase in the spontaneous production of ROS by macrophagal cells with therapeutic concentration of sanazole (0,6-1,25 mM) in the incubation medium was observed. At these concentrations metronidazole had no effect on spontaneous production of ROS by macrophagal cells. PMA-stimulated ROS production was inhibited by high concentrations (2,5-10 mM) of sanazole and metronidazole. The spontaneous generation of ROS by peritoneal macrophages was stimulated by sanazole at all tested concentrations (0,6-10 mM).