S-Nitrosohemoglobin: An allosteric mediator of NO group function in mammalian vasculature

Eric J. Frehm, Joseph Bonaventura, Andrew J. Gow

Research output: Contribution to journalReview article

22 Citations (Scopus)

Abstract

Since the discovery of NO as the endothelium-derived relaxing factor, there has been considerable interest in how NO interacts with hemoglobin (Hb). Numerous investigations have highlighted the possibility that rather than operating as a sink to consume NO, the vasculature can operate as a delivery mechanism for NO. The principal hypothesis proposed to explain this phenomenon is that Hb can transport NO on the conserved cysteine residue β93 and deliver that NO to the tissues in an allosterically dependent manner. This proposal has been termed the S-Nitrosohemoglobin (SNO-Hb) Hypothesis. This review addresses the experimental evidence that led to development of this hypothesis and examines much of the research that resulted from its generation. Specifically it covers the evidence concerning NO in the vasculature, the SNO-Hb Hypothesis itself, the biochemical and biophysical data relating to NO and Hb interactions, SNO-Hb in human physiology, and alternative vascular forms of NO. Finally a model of NO in the vasculature in which SNO-Hb forms the central core is proposed.

Original languageEnglish
Pages (from-to)442-453
Number of pages12
JournalFree Radical Biology and Medicine
Volume37
Issue number4
DOIs
Publication statusPublished - 15 Aug 2004
Externally publishedYes

Keywords

  • Allostery
  • Free radicals
  • Hemoglobin
  • Nitric oxide
  • Nitrosothiol

ASJC Scopus subject areas

  • Medicine(all)
  • Toxicology
  • Clinical Biochemistry

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