Abstract
The response of the human Jurkat T cell leukemia-derived cell line (Jurkat T cells) after 24 h of in vitro exposure to a titanium substrate (12 × 12 × 1 mm3) with a bilateral rough (Ra = 2.2-3.7 μm) titanium oxide coating (rTOC) applied using the micro-arc method in a 20% orthophosphoric acid solution was studied. A 1.5-fold down-regulation of hTERT mRNA expression and decreases in CD3, CD4, CD8, and CD95 presentation and IL-4 and TNFα secretion were observed. Jurkat T cell inactivation was not correlated with the generation of intracellular reactive oxygen species (ROS) and was not mediated by TiO2 nanoparticles with a diameter of 14 ± 8 nm at doses of 1 mg/L or 10 mg/L. The inhibitory effect of the rTOC (Ra = 2.2-3.7 μm) on the survival of Jurkat T cells (Spearman's coefficient rs = -0.95; n = 9; p < 0.0001) was demonstrated by an increase in the necrotic cell count among the cell population. In turn, an elevation of the Ra index of the rTOC was accompanied by a linear increase (r = 0.6; p < 0.000001, n = 60) in the magnitude of the negative electrostatic potential of the titanium oxide surface. Thus, the roughness of the rTOC induces an electrostatic potential and decreases the viability of the immortalized Jurkat T cells through mechanisms unrelated to ROS generation. This may be useful for replacement surgery applications of rough TiO2 implants in cancer patients.
| Original language | English |
|---|---|
| Article number | 360 |
| Journal | Materials |
| Volume | 11 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 28 Feb 2018 |
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Keywords
- In vitro
- Reactive oxygen species
- Surface electrostatic potential
- TiO nanoparticles
- Titanium substrate
- Tumor cell death
ASJC Scopus subject areas
- Materials Science(all)
Cite this
Rough titanium oxide coating prepared by micro-arc oxidation causes down-regulation of hTERT expression, molecular presentation, and cytokine secretion in tumor Jurkat T cells. / Khlusov, Igor; Litvinova, Larisa; Shupletsova, Valeria; Khaziakhmatova, Olga; Melashchenko, Elena; Yurova, Kristina; Leitsin, Vladimir; Khlusova, Marina; Pichugin, Vladimir; Sharkeev, Yurii.
In: Materials, Vol. 11, No. 3, 360, 28.02.2018.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Rough titanium oxide coating prepared by micro-arc oxidation causes down-regulation of hTERT expression, molecular presentation, and cytokine secretion in tumor Jurkat T cells
AU - Khlusov, Igor
AU - Litvinova, Larisa
AU - Shupletsova, Valeria
AU - Khaziakhmatova, Olga
AU - Melashchenko, Elena
AU - Yurova, Kristina
AU - Leitsin, Vladimir
AU - Khlusova, Marina
AU - Pichugin, Vladimir
AU - Sharkeev, Yurii
PY - 2018/2/28
Y1 - 2018/2/28
N2 - The response of the human Jurkat T cell leukemia-derived cell line (Jurkat T cells) after 24 h of in vitro exposure to a titanium substrate (12 × 12 × 1 mm3) with a bilateral rough (Ra = 2.2-3.7 μm) titanium oxide coating (rTOC) applied using the micro-arc method in a 20% orthophosphoric acid solution was studied. A 1.5-fold down-regulation of hTERT mRNA expression and decreases in CD3, CD4, CD8, and CD95 presentation and IL-4 and TNFα secretion were observed. Jurkat T cell inactivation was not correlated with the generation of intracellular reactive oxygen species (ROS) and was not mediated by TiO2 nanoparticles with a diameter of 14 ± 8 nm at doses of 1 mg/L or 10 mg/L. The inhibitory effect of the rTOC (Ra = 2.2-3.7 μm) on the survival of Jurkat T cells (Spearman's coefficient rs = -0.95; n = 9; p < 0.0001) was demonstrated by an increase in the necrotic cell count among the cell population. In turn, an elevation of the Ra index of the rTOC was accompanied by a linear increase (r = 0.6; p < 0.000001, n = 60) in the magnitude of the negative electrostatic potential of the titanium oxide surface. Thus, the roughness of the rTOC induces an electrostatic potential and decreases the viability of the immortalized Jurkat T cells through mechanisms unrelated to ROS generation. This may be useful for replacement surgery applications of rough TiO2 implants in cancer patients.
AB - The response of the human Jurkat T cell leukemia-derived cell line (Jurkat T cells) after 24 h of in vitro exposure to a titanium substrate (12 × 12 × 1 mm3) with a bilateral rough (Ra = 2.2-3.7 μm) titanium oxide coating (rTOC) applied using the micro-arc method in a 20% orthophosphoric acid solution was studied. A 1.5-fold down-regulation of hTERT mRNA expression and decreases in CD3, CD4, CD8, and CD95 presentation and IL-4 and TNFα secretion were observed. Jurkat T cell inactivation was not correlated with the generation of intracellular reactive oxygen species (ROS) and was not mediated by TiO2 nanoparticles with a diameter of 14 ± 8 nm at doses of 1 mg/L or 10 mg/L. The inhibitory effect of the rTOC (Ra = 2.2-3.7 μm) on the survival of Jurkat T cells (Spearman's coefficient rs = -0.95; n = 9; p < 0.0001) was demonstrated by an increase in the necrotic cell count among the cell population. In turn, an elevation of the Ra index of the rTOC was accompanied by a linear increase (r = 0.6; p < 0.000001, n = 60) in the magnitude of the negative electrostatic potential of the titanium oxide surface. Thus, the roughness of the rTOC induces an electrostatic potential and decreases the viability of the immortalized Jurkat T cells through mechanisms unrelated to ROS generation. This may be useful for replacement surgery applications of rough TiO2 implants in cancer patients.
KW - In vitro
KW - Reactive oxygen species
KW - Surface electrostatic potential
KW - TiO nanoparticles
KW - Titanium substrate
KW - Tumor cell death
UR - http://www.scopus.com/inward/record.url?scp=85042693555&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85042693555&partnerID=8YFLogxK
U2 - 10.3390/ma11030360
DO - 10.3390/ma11030360
M3 - Article
AN - SCOPUS:85042693555
VL - 11
JO - Materials
JF - Materials
SN - 1996-1944
IS - 3
M1 - 360
ER -