Rough titanium oxide coating prepared by micro-arc oxidation causes down-regulation of hTERT expression, molecular presentation, and cytokine secretion in tumor Jurkat T cells

Igor Khlusov, Larisa Litvinova, Valeria Shupletsova, Olga Khaziakhmatova, Elena Melashchenko, Kristina Yurova, Vladimir Leitsin, Marina Khlusova, Vladimir Pichugin, Yurii Sharkeev

Research output: Contribution to journalArticle

Abstract

The response of the human Jurkat T cell leukemia-derived cell line (Jurkat T cells) after 24 h of in vitro exposure to a titanium substrate (12 × 12 × 1 mm3) with a bilateral rough (Ra = 2.2-3.7 μm) titanium oxide coating (rTOC) applied using the micro-arc method in a 20% orthophosphoric acid solution was studied. A 1.5-fold down-regulation of hTERT mRNA expression and decreases in CD3, CD4, CD8, and CD95 presentation and IL-4 and TNFα secretion were observed. Jurkat T cell inactivation was not correlated with the generation of intracellular reactive oxygen species (ROS) and was not mediated by TiO2 nanoparticles with a diameter of 14 ± 8 nm at doses of 1 mg/L or 10 mg/L. The inhibitory effect of the rTOC (Ra = 2.2-3.7 μm) on the survival of Jurkat T cells (Spearman's coefficient rs = -0.95; n = 9; p < 0.0001) was demonstrated by an increase in the necrotic cell count among the cell population. In turn, an elevation of the Ra index of the rTOC was accompanied by a linear increase (r = 0.6; p < 0.000001, n = 60) in the magnitude of the negative electrostatic potential of the titanium oxide surface. Thus, the roughness of the rTOC induces an electrostatic potential and decreases the viability of the immortalized Jurkat T cells through mechanisms unrelated to ROS generation. This may be useful for replacement surgery applications of rough TiO2 implants in cancer patients.

Original languageEnglish
Article number360
JournalMaterials
Volume11
Issue number3
DOIs
Publication statusPublished - 28 Feb 2018

Fingerprint

T-cells
Titanium oxides
Tumors
Cells
Cytokines
Coatings
Oxidation
Electrostatics
Reactive Oxygen Species
Orthophosphoric acid
Oxygen
Titanium
Interleukin-4
Surgery
Surface roughness
titanium dioxide
Nanoparticles
Messenger RNA
Substrates

Keywords

  • In vitro
  • Reactive oxygen species
  • Surface electrostatic potential
  • TiO nanoparticles
  • Titanium substrate
  • Tumor cell death

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Rough titanium oxide coating prepared by micro-arc oxidation causes down-regulation of hTERT expression, molecular presentation, and cytokine secretion in tumor Jurkat T cells. / Khlusov, Igor; Litvinova, Larisa; Shupletsova, Valeria; Khaziakhmatova, Olga; Melashchenko, Elena; Yurova, Kristina; Leitsin, Vladimir; Khlusova, Marina; Pichugin, Vladimir; Sharkeev, Yurii.

In: Materials, Vol. 11, No. 3, 360, 28.02.2018.

Research output: Contribution to journalArticle

Khlusov, Igor ; Litvinova, Larisa ; Shupletsova, Valeria ; Khaziakhmatova, Olga ; Melashchenko, Elena ; Yurova, Kristina ; Leitsin, Vladimir ; Khlusova, Marina ; Pichugin, Vladimir ; Sharkeev, Yurii. / Rough titanium oxide coating prepared by micro-arc oxidation causes down-regulation of hTERT expression, molecular presentation, and cytokine secretion in tumor Jurkat T cells. In: Materials. 2018 ; Vol. 11, No. 3.
@article{872b513e9a364a9ba8a25662a37f4415,
title = "Rough titanium oxide coating prepared by micro-arc oxidation causes down-regulation of hTERT expression, molecular presentation, and cytokine secretion in tumor Jurkat T cells",
abstract = "The response of the human Jurkat T cell leukemia-derived cell line (Jurkat T cells) after 24 h of in vitro exposure to a titanium substrate (12 × 12 × 1 mm3) with a bilateral rough (Ra = 2.2-3.7 μm) titanium oxide coating (rTOC) applied using the micro-arc method in a 20{\%} orthophosphoric acid solution was studied. A 1.5-fold down-regulation of hTERT mRNA expression and decreases in CD3, CD4, CD8, and CD95 presentation and IL-4 and TNFα secretion were observed. Jurkat T cell inactivation was not correlated with the generation of intracellular reactive oxygen species (ROS) and was not mediated by TiO2 nanoparticles with a diameter of 14 ± 8 nm at doses of 1 mg/L or 10 mg/L. The inhibitory effect of the rTOC (Ra = 2.2-3.7 μm) on the survival of Jurkat T cells (Spearman's coefficient rs = -0.95; n = 9; p < 0.0001) was demonstrated by an increase in the necrotic cell count among the cell population. In turn, an elevation of the Ra index of the rTOC was accompanied by a linear increase (r = 0.6; p < 0.000001, n = 60) in the magnitude of the negative electrostatic potential of the titanium oxide surface. Thus, the roughness of the rTOC induces an electrostatic potential and decreases the viability of the immortalized Jurkat T cells through mechanisms unrelated to ROS generation. This may be useful for replacement surgery applications of rough TiO2 implants in cancer patients.",
keywords = "In vitro, Reactive oxygen species, Surface electrostatic potential, TiO nanoparticles, Titanium substrate, Tumor cell death",
author = "Igor Khlusov and Larisa Litvinova and Valeria Shupletsova and Olga Khaziakhmatova and Elena Melashchenko and Kristina Yurova and Vladimir Leitsin and Marina Khlusova and Vladimir Pichugin and Yurii Sharkeev",
year = "2018",
month = "2",
day = "28",
doi = "10.3390/ma11030360",
language = "English",
volume = "11",
journal = "Materials",
issn = "1996-1944",
publisher = "MDPI AG",
number = "3",

}

TY - JOUR

T1 - Rough titanium oxide coating prepared by micro-arc oxidation causes down-regulation of hTERT expression, molecular presentation, and cytokine secretion in tumor Jurkat T cells

AU - Khlusov, Igor

AU - Litvinova, Larisa

AU - Shupletsova, Valeria

AU - Khaziakhmatova, Olga

AU - Melashchenko, Elena

AU - Yurova, Kristina

AU - Leitsin, Vladimir

AU - Khlusova, Marina

AU - Pichugin, Vladimir

AU - Sharkeev, Yurii

PY - 2018/2/28

Y1 - 2018/2/28

N2 - The response of the human Jurkat T cell leukemia-derived cell line (Jurkat T cells) after 24 h of in vitro exposure to a titanium substrate (12 × 12 × 1 mm3) with a bilateral rough (Ra = 2.2-3.7 μm) titanium oxide coating (rTOC) applied using the micro-arc method in a 20% orthophosphoric acid solution was studied. A 1.5-fold down-regulation of hTERT mRNA expression and decreases in CD3, CD4, CD8, and CD95 presentation and IL-4 and TNFα secretion were observed. Jurkat T cell inactivation was not correlated with the generation of intracellular reactive oxygen species (ROS) and was not mediated by TiO2 nanoparticles with a diameter of 14 ± 8 nm at doses of 1 mg/L or 10 mg/L. The inhibitory effect of the rTOC (Ra = 2.2-3.7 μm) on the survival of Jurkat T cells (Spearman's coefficient rs = -0.95; n = 9; p < 0.0001) was demonstrated by an increase in the necrotic cell count among the cell population. In turn, an elevation of the Ra index of the rTOC was accompanied by a linear increase (r = 0.6; p < 0.000001, n = 60) in the magnitude of the negative electrostatic potential of the titanium oxide surface. Thus, the roughness of the rTOC induces an electrostatic potential and decreases the viability of the immortalized Jurkat T cells through mechanisms unrelated to ROS generation. This may be useful for replacement surgery applications of rough TiO2 implants in cancer patients.

AB - The response of the human Jurkat T cell leukemia-derived cell line (Jurkat T cells) after 24 h of in vitro exposure to a titanium substrate (12 × 12 × 1 mm3) with a bilateral rough (Ra = 2.2-3.7 μm) titanium oxide coating (rTOC) applied using the micro-arc method in a 20% orthophosphoric acid solution was studied. A 1.5-fold down-regulation of hTERT mRNA expression and decreases in CD3, CD4, CD8, and CD95 presentation and IL-4 and TNFα secretion were observed. Jurkat T cell inactivation was not correlated with the generation of intracellular reactive oxygen species (ROS) and was not mediated by TiO2 nanoparticles with a diameter of 14 ± 8 nm at doses of 1 mg/L or 10 mg/L. The inhibitory effect of the rTOC (Ra = 2.2-3.7 μm) on the survival of Jurkat T cells (Spearman's coefficient rs = -0.95; n = 9; p < 0.0001) was demonstrated by an increase in the necrotic cell count among the cell population. In turn, an elevation of the Ra index of the rTOC was accompanied by a linear increase (r = 0.6; p < 0.000001, n = 60) in the magnitude of the negative electrostatic potential of the titanium oxide surface. Thus, the roughness of the rTOC induces an electrostatic potential and decreases the viability of the immortalized Jurkat T cells through mechanisms unrelated to ROS generation. This may be useful for replacement surgery applications of rough TiO2 implants in cancer patients.

KW - In vitro

KW - Reactive oxygen species

KW - Surface electrostatic potential

KW - TiO nanoparticles

KW - Titanium substrate

KW - Tumor cell death

UR - http://www.scopus.com/inward/record.url?scp=85042693555&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85042693555&partnerID=8YFLogxK

U2 - 10.3390/ma11030360

DO - 10.3390/ma11030360

M3 - Article

VL - 11

JO - Materials

JF - Materials

SN - 1996-1944

IS - 3

M1 - 360

ER -