Role of Cyclic Nucleotides and NO Synthase in Mechanisms of Cardioprotective Effects of Cannabinoid HU-210

L. N. Maslov, A. V. Krylatov, Yu B. Lishmanov

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Isolated perfused rat heart was subjected to global ischemia (45 min) followed by reperfusion (30 min). Under these conditions, the level of creatine phosphokinase in the perfusate increased by 4.5 times. Perfusion (10 min) of the isolated heart with a solution containing cannabinoid HU-210 (0.1 or 1.0 μmol/liter) was followed by a 2-fold decrease in creatine phosphokinase level in the perfusate. The cardioprotective effect of HU-210 remained unchanged under condition of NO synthase inhibition. Cannabinoid HU-210 reduced the concentration of cAMP in the myocardium by 2 times during reperfusion, but did not affect it before and during ischemia. This agent also did not change the level of cGMP in the myocardium before and during ischemia and during reperfusion. The results of the experiment suggest that the cardioprotective effect of HU-210 can be determined by a decrease in cAMP level in the myocardium during reperfusion. cGMP and NO synthase do not contribute to cytoprotective effect of HU-210.

Original languageEnglish
Pages (from-to)588-591
Number of pages4
JournalBulletin of Experimental Biology and Medicine
Volume157
Issue number5
DOIs
Publication statusPublished - 3 Oct 2014

Fingerprint

Cannabinoids
Cyclic Nucleotides
Nitric Oxide Synthase
Reperfusion
Myocardium
Ischemia
Creatine Kinase
Rats
Perfusion
HU 211
Experiments

Keywords

  • cAMP
  • heart
  • HU-210
  • ischemia
  • reperfusion

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Role of Cyclic Nucleotides and NO Synthase in Mechanisms of Cardioprotective Effects of Cannabinoid HU-210. / Maslov, L. N.; Krylatov, A. V.; Lishmanov, Yu B.

In: Bulletin of Experimental Biology and Medicine, Vol. 157, No. 5, 03.10.2014, p. 588-591.

Research output: Contribution to journalArticle

@article{31c17e529f524ad3817840fbc8c5b9c9,
title = "Role of Cyclic Nucleotides and NO Synthase in Mechanisms of Cardioprotective Effects of Cannabinoid HU-210",
abstract = "Isolated perfused rat heart was subjected to global ischemia (45 min) followed by reperfusion (30 min). Under these conditions, the level of creatine phosphokinase in the perfusate increased by 4.5 times. Perfusion (10 min) of the isolated heart with a solution containing cannabinoid HU-210 (0.1 or 1.0 μmol/liter) was followed by a 2-fold decrease in creatine phosphokinase level in the perfusate. The cardioprotective effect of HU-210 remained unchanged under condition of NO synthase inhibition. Cannabinoid HU-210 reduced the concentration of cAMP in the myocardium by 2 times during reperfusion, but did not affect it before and during ischemia. This agent also did not change the level of cGMP in the myocardium before and during ischemia and during reperfusion. The results of the experiment suggest that the cardioprotective effect of HU-210 can be determined by a decrease in cAMP level in the myocardium during reperfusion. cGMP and NO synthase do not contribute to cytoprotective effect of HU-210.",
keywords = "cAMP, heart, HU-210, ischemia, reperfusion",
author = "Maslov, {L. N.} and Krylatov, {A. V.} and Lishmanov, {Yu B.}",
year = "2014",
month = "10",
day = "3",
doi = "10.1007/s10517-014-2622-x",
language = "English",
volume = "157",
pages = "588--591",
journal = "Bulletin of Experimental Biology and Medicine",
issn = "0007-4888",
publisher = "Springer New York",
number = "5",

}

TY - JOUR

T1 - Role of Cyclic Nucleotides and NO Synthase in Mechanisms of Cardioprotective Effects of Cannabinoid HU-210

AU - Maslov, L. N.

AU - Krylatov, A. V.

AU - Lishmanov, Yu B.

PY - 2014/10/3

Y1 - 2014/10/3

N2 - Isolated perfused rat heart was subjected to global ischemia (45 min) followed by reperfusion (30 min). Under these conditions, the level of creatine phosphokinase in the perfusate increased by 4.5 times. Perfusion (10 min) of the isolated heart with a solution containing cannabinoid HU-210 (0.1 or 1.0 μmol/liter) was followed by a 2-fold decrease in creatine phosphokinase level in the perfusate. The cardioprotective effect of HU-210 remained unchanged under condition of NO synthase inhibition. Cannabinoid HU-210 reduced the concentration of cAMP in the myocardium by 2 times during reperfusion, but did not affect it before and during ischemia. This agent also did not change the level of cGMP in the myocardium before and during ischemia and during reperfusion. The results of the experiment suggest that the cardioprotective effect of HU-210 can be determined by a decrease in cAMP level in the myocardium during reperfusion. cGMP and NO synthase do not contribute to cytoprotective effect of HU-210.

AB - Isolated perfused rat heart was subjected to global ischemia (45 min) followed by reperfusion (30 min). Under these conditions, the level of creatine phosphokinase in the perfusate increased by 4.5 times. Perfusion (10 min) of the isolated heart with a solution containing cannabinoid HU-210 (0.1 or 1.0 μmol/liter) was followed by a 2-fold decrease in creatine phosphokinase level in the perfusate. The cardioprotective effect of HU-210 remained unchanged under condition of NO synthase inhibition. Cannabinoid HU-210 reduced the concentration of cAMP in the myocardium by 2 times during reperfusion, but did not affect it before and during ischemia. This agent also did not change the level of cGMP in the myocardium before and during ischemia and during reperfusion. The results of the experiment suggest that the cardioprotective effect of HU-210 can be determined by a decrease in cAMP level in the myocardium during reperfusion. cGMP and NO synthase do not contribute to cytoprotective effect of HU-210.

KW - cAMP

KW - heart

KW - HU-210

KW - ischemia

KW - reperfusion

UR - http://www.scopus.com/inward/record.url?scp=84921938693&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84921938693&partnerID=8YFLogxK

U2 - 10.1007/s10517-014-2622-x

DO - 10.1007/s10517-014-2622-x

M3 - Article

VL - 157

SP - 588

EP - 591

JO - Bulletin of Experimental Biology and Medicine

JF - Bulletin of Experimental Biology and Medicine

SN - 0007-4888

IS - 5

ER -