Role of adiponectin and proinflammatory gene expression in adipose tissue chronic inflammation in women with metabolic syndrome

Larisa Litvinova, Dmitriy Atochin, Mariia Vasilenko, Nikolai Fattakhov, Pavel Zatolokin, Igor Vaysbeyn, Elena Kirienkova

Research output: Contribution to journalArticle

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Abstract

Background: The purpose of this research was to study the gene expression of interleukin-6 (IL-6), tumor necrosis factor-α(TNF-α), nuclear factor kappa B (NF-κB), vascular endothelial growth factor A (VEGF-A) and adiponectin (AdipoQ) genes in the visceral (omental, mesenteric) and subcutaneous adipose tissue depots in metabolic syndrome (MS). We studied 23 women with MS, with a mean age of 50.7 ± 4.5 years and mean body mass index (BMI) of 45.6 ± 9.8 kg/m2. The control group included 10 women, with a mean age of 40.6 ± 8.7 years and normal BMI (22.3 ± 3.7 kg/m2). The gene expression levels in the omental (OAT), mesenteric (MAT) and subcutaneous (SAT) adipose tissues were assessed by quantitative real-time PCR. Findings: Increased gene expression levels of IL-6 and TNF-α were detected in MAT in patients with MS, compared with the control group (p <0.05 and p <0.005, respectively). Significant positive correlations were observed between IL-6 mRNA expression levels in OAT and the content of CD14 + cells in the peripheral blood (r = 0.55, p <0.05), as well as between NF-κB and VEGF-A mRNA levels in OAT (r = 0.43, p <0.05) in patients with MS. The AdipoQ gene expression levels in OAT were significantly decreased in women with MS compared with the control group (p <0.05). In addition, there were inverse correlations between AdipoQ gene levels in MAT and serum CRP levels (r = -0.63, p <0.05), as well as between AdipoQ gene levels in MAT and serum IL-6 levels (r = -0.46, p <0.05). Conclusion: These data demonstrate that proinflammatory gene expression of MAT in women with MS was increased compared with the control group. The AdipoQ gene expression levels in OAT were significantly decreased in women with MS compared with the control group.

Original languageEnglish
Article number137
JournalDiabetology and Metabolic Syndrome
Volume6
Issue number1
DOIs
Publication statusPublished - 2014
Externally publishedYes

Fingerprint

Adiponectin
Adipose Tissue
Inflammation
Gene Expression
Interleukin-6
Control Groups
NF-kappa B
Subcutaneous Fat
Vascular Endothelial Growth Factor A
Body Mass Index
Genes
Messenger RNA
Intra-Abdominal Fat
Serum
Real-Time Polymerase Chain Reaction
Tumor Necrosis Factor-alpha
Research

Keywords

  • Adipokine
  • Adipose tissue
  • Gene expression
  • Inflammation
  • Metabolic syndrome
  • Obesity

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Internal Medicine

Cite this

Role of adiponectin and proinflammatory gene expression in adipose tissue chronic inflammation in women with metabolic syndrome. / Litvinova, Larisa; Atochin, Dmitriy; Vasilenko, Mariia; Fattakhov, Nikolai; Zatolokin, Pavel; Vaysbeyn, Igor; Kirienkova, Elena.

In: Diabetology and Metabolic Syndrome, Vol. 6, No. 1, 137, 2014.

Research output: Contribution to journalArticle

Litvinova, Larisa ; Atochin, Dmitriy ; Vasilenko, Mariia ; Fattakhov, Nikolai ; Zatolokin, Pavel ; Vaysbeyn, Igor ; Kirienkova, Elena. / Role of adiponectin and proinflammatory gene expression in adipose tissue chronic inflammation in women with metabolic syndrome. In: Diabetology and Metabolic Syndrome. 2014 ; Vol. 6, No. 1.
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AU - Litvinova, Larisa

AU - Atochin, Dmitriy

AU - Vasilenko, Mariia

AU - Fattakhov, Nikolai

AU - Zatolokin, Pavel

AU - Vaysbeyn, Igor

AU - Kirienkova, Elena

PY - 2014

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N2 - Background: The purpose of this research was to study the gene expression of interleukin-6 (IL-6), tumor necrosis factor-α(TNF-α), nuclear factor kappa B (NF-κB), vascular endothelial growth factor A (VEGF-A) and adiponectin (AdipoQ) genes in the visceral (omental, mesenteric) and subcutaneous adipose tissue depots in metabolic syndrome (MS). We studied 23 women with MS, with a mean age of 50.7 ± 4.5 years and mean body mass index (BMI) of 45.6 ± 9.8 kg/m2. The control group included 10 women, with a mean age of 40.6 ± 8.7 years and normal BMI (22.3 ± 3.7 kg/m2). The gene expression levels in the omental (OAT), mesenteric (MAT) and subcutaneous (SAT) adipose tissues were assessed by quantitative real-time PCR. Findings: Increased gene expression levels of IL-6 and TNF-α were detected in MAT in patients with MS, compared with the control group (p <0.05 and p <0.005, respectively). Significant positive correlations were observed between IL-6 mRNA expression levels in OAT and the content of CD14 + cells in the peripheral blood (r = 0.55, p <0.05), as well as between NF-κB and VEGF-A mRNA levels in OAT (r = 0.43, p <0.05) in patients with MS. The AdipoQ gene expression levels in OAT were significantly decreased in women with MS compared with the control group (p <0.05). In addition, there were inverse correlations between AdipoQ gene levels in MAT and serum CRP levels (r = -0.63, p <0.05), as well as between AdipoQ gene levels in MAT and serum IL-6 levels (r = -0.46, p <0.05). Conclusion: These data demonstrate that proinflammatory gene expression of MAT in women with MS was increased compared with the control group. The AdipoQ gene expression levels in OAT were significantly decreased in women with MS compared with the control group.

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