Role of κ1 opioid receptors and cAMP in regulation of cardiac tolerance to ischemia and reperfusion

T. V. Lasukova, L. N. Maslov, A. A. Platonov, N. V. Guzarova, Yu B. Lishmanov

Research output: Contribution to journalArticle

Abstract

The cardioprotective, inotropic, and antiarrhythmic effects of U-50.488, a selective agonist of κ1 opioid receptors (κ1 ORs), was studied using the model of 45-min total ischemia and 30-min reperfusion of isolated rat heart. Cardiac κ1 ORs were stimulated by adding U-50.488 to the perfusing solution up to the final concentration of 0.1 or 1 μmol/l. The opioid had no influence on the incidence of reperfusion arrhythmias. The addition of 0.1 μmol/l U-50.488 reduced the reperfusion release of creatine phosphokinase (CPK) by half, which positively correlated with the decrease in the myocardial cAMP content (r = 0.89, p < 0.01). At the same time, the addition of U-50.488 in the higher concentration (1 μmol/l) had no effect on either cAMP level or CPK release. These results indicate that the cardioprotective effect of U-50.488 may be connected with the reduction of myocardial cAMP content. Activation of κ1 ORs caused a decrease in both frequency and amplitude of myocardial contractions. The negative inotropic and chronotropic effect of U-50.488 was shown to be independent of changes in the myocardial cAMP content. A hypothesis is proposed that the absence of any cardioprotective effect of U-50.488 at the higher concentration (1 μmol/l) is accounted for by its interaction with unknown nonopioid receptors of cardiac myocytes.

Original languageEnglish
Pages (from-to)194-201
Number of pages8
JournalBiology Bulletin
Volume35
Issue number2
DOIs
Publication statusPublished - 1 Apr 2008

Fingerprint

narcotics
Opioid Receptors
ischemia
Reperfusion
Ischemia
Creatine Kinase
cardioprotective effect
receptors
creatine kinase
Myocardial Contraction
Cardiac Myocytes
Opioid Analgesics
Rats
Cardiac Arrhythmias
arrhythmia
Chemical activation
agonists
heart
Incidence
incidence

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Role of κ1 opioid receptors and cAMP in regulation of cardiac tolerance to ischemia and reperfusion. / Lasukova, T. V.; Maslov, L. N.; Platonov, A. A.; Guzarova, N. V.; Lishmanov, Yu B.

In: Biology Bulletin, Vol. 35, No. 2, 01.04.2008, p. 194-201.

Research output: Contribution to journalArticle

Lasukova, T. V. ; Maslov, L. N. ; Platonov, A. A. ; Guzarova, N. V. ; Lishmanov, Yu B. / Role of κ1 opioid receptors and cAMP in regulation of cardiac tolerance to ischemia and reperfusion. In: Biology Bulletin. 2008 ; Vol. 35, No. 2. pp. 194-201.
@article{4caba0bc42a643cf8d48d43845e36042,
title = "Role of κ1 opioid receptors and cAMP in regulation of cardiac tolerance to ischemia and reperfusion",
abstract = "The cardioprotective, inotropic, and antiarrhythmic effects of U-50.488, a selective agonist of κ1 opioid receptors (κ1 ORs), was studied using the model of 45-min total ischemia and 30-min reperfusion of isolated rat heart. Cardiac κ1 ORs were stimulated by adding U-50.488 to the perfusing solution up to the final concentration of 0.1 or 1 μmol/l. The opioid had no influence on the incidence of reperfusion arrhythmias. The addition of 0.1 μmol/l U-50.488 reduced the reperfusion release of creatine phosphokinase (CPK) by half, which positively correlated with the decrease in the myocardial cAMP content (r = 0.89, p < 0.01). At the same time, the addition of U-50.488 in the higher concentration (1 μmol/l) had no effect on either cAMP level or CPK release. These results indicate that the cardioprotective effect of U-50.488 may be connected with the reduction of myocardial cAMP content. Activation of κ1 ORs caused a decrease in both frequency and amplitude of myocardial contractions. The negative inotropic and chronotropic effect of U-50.488 was shown to be independent of changes in the myocardial cAMP content. A hypothesis is proposed that the absence of any cardioprotective effect of U-50.488 at the higher concentration (1 μmol/l) is accounted for by its interaction with unknown nonopioid receptors of cardiac myocytes.",
author = "Lasukova, {T. V.} and Maslov, {L. N.} and Platonov, {A. A.} and Guzarova, {N. V.} and Lishmanov, {Yu B.}",
year = "2008",
month = "4",
day = "1",
doi = "10.1007/s10525-008-2014-8",
language = "English",
volume = "35",
pages = "194--201",
journal = "Biology Bulletin",
issn = "1062-3590",
publisher = "Maik Nauka-Interperiodica Publishing",
number = "2",

}

TY - JOUR

T1 - Role of κ1 opioid receptors and cAMP in regulation of cardiac tolerance to ischemia and reperfusion

AU - Lasukova, T. V.

AU - Maslov, L. N.

AU - Platonov, A. A.

AU - Guzarova, N. V.

AU - Lishmanov, Yu B.

PY - 2008/4/1

Y1 - 2008/4/1

N2 - The cardioprotective, inotropic, and antiarrhythmic effects of U-50.488, a selective agonist of κ1 opioid receptors (κ1 ORs), was studied using the model of 45-min total ischemia and 30-min reperfusion of isolated rat heart. Cardiac κ1 ORs were stimulated by adding U-50.488 to the perfusing solution up to the final concentration of 0.1 or 1 μmol/l. The opioid had no influence on the incidence of reperfusion arrhythmias. The addition of 0.1 μmol/l U-50.488 reduced the reperfusion release of creatine phosphokinase (CPK) by half, which positively correlated with the decrease in the myocardial cAMP content (r = 0.89, p < 0.01). At the same time, the addition of U-50.488 in the higher concentration (1 μmol/l) had no effect on either cAMP level or CPK release. These results indicate that the cardioprotective effect of U-50.488 may be connected with the reduction of myocardial cAMP content. Activation of κ1 ORs caused a decrease in both frequency and amplitude of myocardial contractions. The negative inotropic and chronotropic effect of U-50.488 was shown to be independent of changes in the myocardial cAMP content. A hypothesis is proposed that the absence of any cardioprotective effect of U-50.488 at the higher concentration (1 μmol/l) is accounted for by its interaction with unknown nonopioid receptors of cardiac myocytes.

AB - The cardioprotective, inotropic, and antiarrhythmic effects of U-50.488, a selective agonist of κ1 opioid receptors (κ1 ORs), was studied using the model of 45-min total ischemia and 30-min reperfusion of isolated rat heart. Cardiac κ1 ORs were stimulated by adding U-50.488 to the perfusing solution up to the final concentration of 0.1 or 1 μmol/l. The opioid had no influence on the incidence of reperfusion arrhythmias. The addition of 0.1 μmol/l U-50.488 reduced the reperfusion release of creatine phosphokinase (CPK) by half, which positively correlated with the decrease in the myocardial cAMP content (r = 0.89, p < 0.01). At the same time, the addition of U-50.488 in the higher concentration (1 μmol/l) had no effect on either cAMP level or CPK release. These results indicate that the cardioprotective effect of U-50.488 may be connected with the reduction of myocardial cAMP content. Activation of κ1 ORs caused a decrease in both frequency and amplitude of myocardial contractions. The negative inotropic and chronotropic effect of U-50.488 was shown to be independent of changes in the myocardial cAMP content. A hypothesis is proposed that the absence of any cardioprotective effect of U-50.488 at the higher concentration (1 μmol/l) is accounted for by its interaction with unknown nonopioid receptors of cardiac myocytes.

UR - http://www.scopus.com/inward/record.url?scp=43349103089&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=43349103089&partnerID=8YFLogxK

U2 - 10.1007/s10525-008-2014-8

DO - 10.1007/s10525-008-2014-8

M3 - Article

VL - 35

SP - 194

EP - 201

JO - Biology Bulletin

JF - Biology Bulletin

SN - 1062-3590

IS - 2

ER -