Ribonuclease from Bacillus thuringiensis var. subtoxicus: Gene structure and regulation of biosynthesis

A. A. Shulga, L. V. Znamenskaya, O. V. Morozova, I. B. Leshchinskaya, M. P. Kirpichnikov

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


The gene for extracellular guanyl-specific ribonuclease of Bacillus thuringiensis var. subtoxicus (RNase Bth), a close homologue of the B. intermedius RNase (binase), was completely sequenced. Analysis of nucleotide sequences in the regions adjoining RNase genes revealed an identical organization of the chromosomal loci of RNase Bth and binase. Growth characteristics of the Bacillus thuringiensis var. subtoxicus strain and its synthesis of RNase were studied. It was shown that the exogenous inorganic phosphate inhibits the biosynthesis of RNase. At the same time, actinomycin D in low doses stimulates the enzyme synthesis. Comparative analysis of the influence of inorganic phosphate and actinomycin D on the biosynthesis of RNAse Bth and binase suggests a possibility of coincidence of regulatory pathways of synthesis of these enzymes.

Original languageEnglish
Pages (from-to)672-678
Number of pages7
JournalBioorganicheskaya Khimiya
Issue number9
Publication statusPublished - 2000
Externally publishedYes


  • Bacillus thuringiensis
  • Biosynthesis, repression
  • Gene sequence
  • Inorganic phosphate
  • Ribonuclease

ASJC Scopus subject areas

  • Medicine(all)

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