Riboflavin photoactivation by upconversion nanoparticles for cancer treatment

E. V. Khaydukov, K. E. Mironova, V. A. Semchishen, A. N. Generalova, A. V. Nechaev, D. A. Khochenkov, E. V. Stepanova, O. I. Lebedev, A. V. Zvyagin, S. M. Deyev, V. Ya Panchenko

Research output: Contribution to journalArticle

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Abstract

Riboflavin (Rf) is a vitamin and endogenous photosensitizer capable to generate reactive oxygen species (ROS) under UV-blue irradiation and kill cancer cells, which are characterized by the enhanced uptake of Rf. We confirmed its phototoxicity on human breast adenocarcinoma cells SK-BR-3 preincubated with 30-μM Rf and irradiated with ultraviolet light, and proved that such Rf concentrations (60 μM) are attainable in vivo in tumour site by systemic intravascular injection. In order to extend the Rf photosensitization depth in cancer tissue to 6 mm in depth, we purpose-designed core/shell upconversion nanoparticles (UCNPs, NaYF4:Yb3+:Tm3+/NaYF4) capable to convert 2% of the deeply-penetrating excitation at 975 nm to ultraviolet-blue power. This power was expended to photosensitise Rf and kill SK-BR-3 cells preincubated with UCNPs and Rf, where the UCNP-Rf energy transfer was photon-mediated with ∼14% Förster process contribution. SK-BR-3 xenograft regression in mice was observed for 50 days, following the Rf-UCNPs peritumoural injection and near-infrared light photodynamic treatment of the lesions.

Original languageEnglish
Article number35103
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 12 Oct 2016

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Riboflavin
Nanoparticles
Neoplasms
Therapeutics
Phototoxic Dermatitis
Photosensitivity Disorders
Injections
Photosensitizing Agents
Energy Transfer
Ultraviolet Rays
Photons
Heterografts
Vitamins
Reactive Oxygen Species
Adenocarcinoma
Breast
Light

ASJC Scopus subject areas

  • General

Cite this

Khaydukov, E. V., Mironova, K. E., Semchishen, V. A., Generalova, A. N., Nechaev, A. V., Khochenkov, D. A., ... Panchenko, V. Y. (2016). Riboflavin photoactivation by upconversion nanoparticles for cancer treatment. Scientific Reports, 6, [35103]. https://doi.org/10.1038/srep35103

Riboflavin photoactivation by upconversion nanoparticles for cancer treatment. / Khaydukov, E. V.; Mironova, K. E.; Semchishen, V. A.; Generalova, A. N.; Nechaev, A. V.; Khochenkov, D. A.; Stepanova, E. V.; Lebedev, O. I.; Zvyagin, A. V.; Deyev, S. M.; Panchenko, V. Ya.

In: Scientific Reports, Vol. 6, 35103, 12.10.2016.

Research output: Contribution to journalArticle

Khaydukov, EV, Mironova, KE, Semchishen, VA, Generalova, AN, Nechaev, AV, Khochenkov, DA, Stepanova, EV, Lebedev, OI, Zvyagin, AV, Deyev, SM & Panchenko, VY 2016, 'Riboflavin photoactivation by upconversion nanoparticles for cancer treatment', Scientific Reports, vol. 6, 35103. https://doi.org/10.1038/srep35103
Khaydukov EV, Mironova KE, Semchishen VA, Generalova AN, Nechaev AV, Khochenkov DA et al. Riboflavin photoactivation by upconversion nanoparticles for cancer treatment. Scientific Reports. 2016 Oct 12;6. 35103. https://doi.org/10.1038/srep35103
Khaydukov, E. V. ; Mironova, K. E. ; Semchishen, V. A. ; Generalova, A. N. ; Nechaev, A. V. ; Khochenkov, D. A. ; Stepanova, E. V. ; Lebedev, O. I. ; Zvyagin, A. V. ; Deyev, S. M. ; Panchenko, V. Ya. / Riboflavin photoactivation by upconversion nanoparticles for cancer treatment. In: Scientific Reports. 2016 ; Vol. 6.
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