Reduced ischemia and reperfusion injury following exercise training

Joseph R. Libonati, John P. Gaughan, Colleen A. Hefner, Andrew Gow, Albert M. Paolone, Steven R. Houser

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Abstract

We examined the effects of two exercise training modalities, i.e., low intensity endurance and sprint running, on in vitro, isovolumic myocardial performance following ischemia and reperfusion. Rats ran on a treadmill 5 d · wk-1 for 6 wk at the following levels: endurance; 20 m · min-1, 0% grade, 60 min · d-1 and sprint; five 1-min runs at 75 m · min-1, 15% grade interspersed with 1-min active recovery runs at 20 m · min-1, 15% grade. Both endurance and sprint training significantly improved exercise tolerance relative to control (P <0.05) on two graded exercise tests. Buffer perfused hearts of control (N = 18), endurance (N = 20), and sprint (N = 13) trained animals underwent no-flow ischemia (20 min) and reperfusion (30 min) in a Langendorff mode. During reperfusion, left ventricular developed pressure and its first derivative were 20% higher in sprint (P <0.05) than either endurance or control hearts. Left ventricular end-diastolic pressure was lowest in sprint during reperfusion (sprint, 10 ± 1 mm Hg vs endurance, 14 ± 2 mm Hg; and control, 14 ± 2 mm Hg, at 30 min reperfusion). Hearts were then used for biochemical studies or dissociated into single cells for measurement of contraction, cell calcium, and action potential duration. Single cell contractions were greatest in sprint despite similar calcium transients in all groups. Ischemia/reperfusion caused action potential prolongation in control but not trained myocytes. Hearts from sprint had the greatest glyceraldehyde-3-phosphate dehydrogenase activity (P <0.05) and a tendency towards increased superoxide dismutase activity. These results suggest that sprinting increases myocardial resistance to ischemia/reperfusion. This protection may be secondary to increased myofilament calcium sensitivity and/or myocardial expression of glyceraldehyde-3-phosphate dehydrogenase.

Original languageEnglish
Pages (from-to)509-516
Number of pages8
JournalMedicine and Science in Sports and Exercise
Volume29
Issue number4
Publication statusPublished - 1997
Externally publishedYes

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Keywords

  • CALCIUM
  • ELECTROPHYSIOLOGY
  • ISOLATED HEARTS
  • MYOCYTES
  • RATS

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Physical Therapy, Sports Therapy and Rehabilitation
  • Orthopedics and Sports Medicine

Cite this

Libonati, J. R., Gaughan, J. P., Hefner, C. A., Gow, A., Paolone, A. M., & Houser, S. R. (1997). Reduced ischemia and reperfusion injury following exercise training. Medicine and Science in Sports and Exercise, 29(4), 509-516.