Abstract
Recombinant barnase was proposed as a label in the enzyme-linked immunosorbent assay (ELISA). Barnase-conjugated pig transferrin was prepared by the periodate oxidation procedure. Solid-phase bound barnase activity was determined from the change in RNA-ethidium bromide complex fluorescence upon RNA hydrolysis. The sensitivity of transferrin-barnase conjugate determination in ELISA was no less than 5 ng per well. The conjugate was applied in competition ELISA for free transferrin determination.
Original language | English |
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Pages (from-to) | 99-102 |
Number of pages | 4 |
Journal | FEBS Letters |
Volume | 388 |
Issue number | 2-3 |
DOIs | |
Publication status | Published - 17 Jun 1996 |
Externally published | Yes |
Keywords
- Barnase
- ELISA
- Ethidium bromide
- Fluorescence
- Monoclonal antibodies
- Transferrin
ASJC Scopus subject areas
- Structural Biology
- Biophysics
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology