Recombinant barnase as a label in ELISA

Sergey A. Yazynin, Sergey M. Deyev

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Recombinant barnase was proposed as a label in the enzyme-linked immunosorbent assay (ELISA). Barnase-conjugated pig transferrin was prepared by the periodate oxidation procedure. Solid-phase bound barnase activity was determined from the change in RNA-ethidium bromide complex fluorescence upon RNA hydrolysis. The sensitivity of transferrin-barnase conjugate determination in ELISA was no less than 5 ng per well. The conjugate was applied in competition ELISA for free transferrin determination.

Original languageEnglish
Pages (from-to)99-102
Number of pages4
JournalFEBS Letters
Volume388
Issue number2-3
DOIs
Publication statusPublished - 17 Jun 1996
Externally publishedYes

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Keywords

  • Barnase
  • ELISA
  • Ethidium bromide
  • Fluorescence
  • Monoclonal antibodies
  • Transferrin

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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