Recombinant barnase as a label in ELISA

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Abstract

Recombinant barnase was proposed as a label in the enzyme-linked immunosorbent assay (ELISA). Barnase-conjugated pig transferrin was prepared by the periodate oxidation procedure. Solid-phase bound barnase activity was determined from the change in RNA-ethidium bromide complex fluorescence upon RNA hydrolysis. The sensitivity of transferrin-barnase conjugate determination in ELISA was no less than 5 ng per well. The conjugate was applied in competition ELISA for free transferrin determination.

Original language	English
Pages (from-to)	99-102
Number of pages	4
Journal	FEBS Letters
Volume	388
Issue number	2-3
DOIs	https://doi.org/10.1016/0014-5793(96)00535-2
Publication status	Published - 17 Jun 1996
Externally published	Yes

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Keywords

Barnase
ELISA
Ethidium bromide
Fluorescence
Monoclonal antibodies
Transferrin

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Molecular Biology
Genetics
Cell Biology

Cite this

Yazynin, S. A., & Deyev, S. M. (1996). Recombinant barnase as a label in ELISA. FEBS Letters, 388(2-3), 99-102. https://doi.org/10.1016/0014-5793(96)00535-2

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KW - Ethidium bromide

KW - Fluorescence

KW - Monoclonal antibodies

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Access to Document

10.1016/0014-5793(96)00535-2