Recombinant barnase as a label in ELISA

Abstract

Recombinant barnase was proposed as a label in the enzyme-linked immunosorbent assay (ELISA). Barnase-conjugated pig transferrin was prepared by the periodate oxidation procedure. Solid-phase bound barnase activity was determined from the change in RNA-ethidium bromide complex fluorescence upon RNA hydrolysis. The sensitivity of transferrin-barnase conjugate determination in ELISA was no less than 5 ng per well. The conjugate was applied in competition ELISA for free transferrin determination.

Original language	English
Pages (from-to)	99-102
Number of pages	4
Journal	FEBS Letters
Volume	388
Issue number	2-3
DOIs	https://doi.org/10.1016/0014-5793(96)00535-2
Publication status	Published - 17 Jun 1996
Externally published	Yes

Keywords

Barnase
ELISA
Ethidium bromide
Fluorescence
Monoclonal antibodies
Transferrin

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

10.1016/0014-5793(96)00535-2

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title = "Recombinant barnase as a label in ELISA",

abstract = "Recombinant barnase was proposed as a label in the enzyme-linked immunosorbent assay (ELISA). Barnase-conjugated pig transferrin was prepared by the periodate oxidation procedure. Solid-phase bound barnase activity was determined from the change in RNA-ethidium bromide complex fluorescence upon RNA hydrolysis. The sensitivity of transferrin-barnase conjugate determination in ELISA was no less than 5 ng per well. The conjugate was applied in competition ELISA for free transferrin determination.",

keywords = "Barnase, ELISA, Ethidium bromide, Fluorescence, Monoclonal antibodies, Transferrin",

author = "Yazynin, {Sergey A.} and Deyev, {Sergey M.}",

year = "1996",

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doi = "10.1016/0014-5793(96)00535-2",

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pages = "99--102",

journal = "FEBS Letters",

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T1 - Recombinant barnase as a label in ELISA

AU - Yazynin, Sergey A.

AU - Deyev, Sergey M.

PY - 1996/6/17

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N2 - Recombinant barnase was proposed as a label in the enzyme-linked immunosorbent assay (ELISA). Barnase-conjugated pig transferrin was prepared by the periodate oxidation procedure. Solid-phase bound barnase activity was determined from the change in RNA-ethidium bromide complex fluorescence upon RNA hydrolysis. The sensitivity of transferrin-barnase conjugate determination in ELISA was no less than 5 ng per well. The conjugate was applied in competition ELISA for free transferrin determination.

AB - Recombinant barnase was proposed as a label in the enzyme-linked immunosorbent assay (ELISA). Barnase-conjugated pig transferrin was prepared by the periodate oxidation procedure. Solid-phase bound barnase activity was determined from the change in RNA-ethidium bromide complex fluorescence upon RNA hydrolysis. The sensitivity of transferrin-barnase conjugate determination in ELISA was no less than 5 ng per well. The conjugate was applied in competition ELISA for free transferrin determination.

KW - Barnase

KW - ELISA

KW - Ethidium bromide

KW - Fluorescence

KW - Monoclonal antibodies

KW - Transferrin

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