Abstract
Recombinant barnase was proposed as a label in the enzyme-linked immunosorbent assay (ELISA). Barnase-conjugated pig transferrin was prepared by the periodate oxidation procedure. Solid-phase bound barnase activity was determined from the change in RNA-ethidium bromide complex fluorescence upon RNA hydrolysis. The sensitivity of transferrin-barnase conjugate determination in ELISA was no less than 5 ng per well. The conjugate was applied in competition ELISA for free transferrin determination.
| Original language | English |
|---|---|
| Pages (from-to) | 99-102 |
| Number of pages | 4 |
| Journal | FEBS Letters |
| Volume | 388 |
| Issue number | 2-3 |
| DOIs | |
| Publication status | Published - 17 Jun 1996 |
| Externally published | Yes |
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Keywords
- Barnase
- ELISA
- Ethidium bromide
- Fluorescence
- Monoclonal antibodies
- Transferrin
ASJC Scopus subject areas
- Structural Biology
- Biophysics
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology
Cite this
Recombinant barnase as a label in ELISA. / Yazynin, Sergey A.; Deyev, Sergey M.
In: FEBS Letters, Vol. 388, No. 2-3, 17.06.1996, p. 99-102.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Recombinant barnase as a label in ELISA
AU - Yazynin, Sergey A.
AU - Deyev, Sergey M.
PY - 1996/6/17
Y1 - 1996/6/17
N2 - Recombinant barnase was proposed as a label in the enzyme-linked immunosorbent assay (ELISA). Barnase-conjugated pig transferrin was prepared by the periodate oxidation procedure. Solid-phase bound barnase activity was determined from the change in RNA-ethidium bromide complex fluorescence upon RNA hydrolysis. The sensitivity of transferrin-barnase conjugate determination in ELISA was no less than 5 ng per well. The conjugate was applied in competition ELISA for free transferrin determination.
AB - Recombinant barnase was proposed as a label in the enzyme-linked immunosorbent assay (ELISA). Barnase-conjugated pig transferrin was prepared by the periodate oxidation procedure. Solid-phase bound barnase activity was determined from the change in RNA-ethidium bromide complex fluorescence upon RNA hydrolysis. The sensitivity of transferrin-barnase conjugate determination in ELISA was no less than 5 ng per well. The conjugate was applied in competition ELISA for free transferrin determination.
KW - Barnase
KW - ELISA
KW - Ethidium bromide
KW - Fluorescence
KW - Monoclonal antibodies
KW - Transferrin
UR - http://www.scopus.com/inward/record.url?scp=0030591453&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030591453&partnerID=8YFLogxK
U2 - 10.1016/0014-5793(96)00535-2
DO - 10.1016/0014-5793(96)00535-2
M3 - Article
VL - 388
SP - 99
EP - 102
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 2-3
ER -