Reaction rates in enzymatic assay system in solutions of metal salts and humic substances

Anna S. Tarasova, Nadezhda S. Kudryasheva

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

This chapter considers the effects of humic substances (HS) on solutions of model inorganic pollutants, metal salts - K₃[Fe(CN)₆] and CuSO₄. Bioluminescent system of coupled enzymatic reactions catalyzed by NAD(P)H-FMN oxidoreductase and bacterial luciferase was used as a bioassay to monitor toxicity of salt solutions in the presence and absence of HS. Ability of HS to decrease or increase general toxicity of the solutions was revealed. The rates of redox reaction in bioluminescent assay system were analyzed in the absence and presence of HS. Acceleration of NADH auto-oxidation by HS was supposed to result in toxicity increase. Acceleration of endogenous NADH-dependent redox reactions in the bioluminescent assay system is supposed to be responsible for detoxification of the salt solutions.

Original language	English
Title of host publication	Functions of Natural Organic Matter in Changing Environment
Publisher	Springer Netherlands
Pages	561-565
Number of pages	5
Volume	9789400756342
ISBN (Electronic)	9789400756342
ISBN (Print)	940075633X, 9789400756335
DOIs	https://doi.org/10.1007/978-94-007-5634-2_101
Publication status	Published - 1 Aug 2013
Externally published	Yes

Fingerprint

Keywords

Bioluminescent assay
Enzyme reaction rates
Humic substances
Metal salts
Toxicity

ASJC Scopus subject areas

Agricultural and Biological Sciences(all)
Environmental Science(all)
Engineering(all)

Cite this

Tarasova, A. S., & Kudryasheva, N. S. (2013). Reaction rates in enzymatic assay system in solutions of metal salts and humic substances. In Functions of Natural Organic Matter in Changing Environment (Vol. 9789400756342, pp. 561-565). Springer Netherlands. https://doi.org/10.1007/978-94-007-5634-2_101

@inbook{4a9312ebef464d5db5c4cd79fc82aa5a,

title = "Reaction rates in enzymatic assay system in solutions of metal salts and humic substances",

abstract = "This chapter considers the effects of humic substances (HS) on solutions of model inorganic pollutants, metal salts - K3[Fe(CN)6] and CuSO4. Bioluminescent system of coupled enzymatic reactions catalyzed by NAD(P)H-FMN oxidoreductase and bacterial luciferase was used as a bioassay to monitor toxicity of salt solutions in the presence and absence of HS. Ability of HS to decrease or increase general toxicity of the solutions was revealed. The rates of redox reaction in bioluminescent assay system were analyzed in the absence and presence of HS. Acceleration of NADH auto-oxidation by HS was supposed to result in toxicity increase. Acceleration of endogenous NADH-dependent redox reactions in the bioluminescent assay system is supposed to be responsible for detoxification of the salt solutions.",

keywords = "Bioluminescent assay, Enzyme reaction rates, Humic substances, Metal salts, Toxicity",

author = "Tarasova, {Anna S.} and Kudryasheva, {Nadezhda S.}",

year = "2013",

month = "8",

day = "1",

doi = "10.1007/978-94-007-5634-2_101",

language = "English",

isbn = "940075633X",

volume = "9789400756342",

pages = "561--565",

booktitle = "Functions of Natural Organic Matter in Changing Environment",

publisher = "Springer Netherlands",

address = "Netherlands",

}

TY - CHAP

T1 - Reaction rates in enzymatic assay system in solutions of metal salts and humic substances

AU - Tarasova, Anna S.

AU - Kudryasheva, Nadezhda S.

PY - 2013/8/1

Y1 - 2013/8/1

N2 - This chapter considers the effects of humic substances (HS) on solutions of model inorganic pollutants, metal salts - K3[Fe(CN)6] and CuSO4. Bioluminescent system of coupled enzymatic reactions catalyzed by NAD(P)H-FMN oxidoreductase and bacterial luciferase was used as a bioassay to monitor toxicity of salt solutions in the presence and absence of HS. Ability of HS to decrease or increase general toxicity of the solutions was revealed. The rates of redox reaction in bioluminescent assay system were analyzed in the absence and presence of HS. Acceleration of NADH auto-oxidation by HS was supposed to result in toxicity increase. Acceleration of endogenous NADH-dependent redox reactions in the bioluminescent assay system is supposed to be responsible for detoxification of the salt solutions.

AB - This chapter considers the effects of humic substances (HS) on solutions of model inorganic pollutants, metal salts - K3[Fe(CN)6] and CuSO4. Bioluminescent system of coupled enzymatic reactions catalyzed by NAD(P)H-FMN oxidoreductase and bacterial luciferase was used as a bioassay to monitor toxicity of salt solutions in the presence and absence of HS. Ability of HS to decrease or increase general toxicity of the solutions was revealed. The rates of redox reaction in bioluminescent assay system were analyzed in the absence and presence of HS. Acceleration of NADH auto-oxidation by HS was supposed to result in toxicity increase. Acceleration of endogenous NADH-dependent redox reactions in the bioluminescent assay system is supposed to be responsible for detoxification of the salt solutions.

KW - Bioluminescent assay

KW - Enzyme reaction rates

KW - Humic substances

KW - Metal salts

KW - Toxicity

UR - http://www.scopus.com/inward/record.url?scp=84932615554&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84932615554&partnerID=8YFLogxK

U2 - 10.1007/978-94-007-5634-2_101

DO - 10.1007/978-94-007-5634-2_101

M3 - Chapter

AN - SCOPUS:84932615554

SN - 940075633X

SN - 9789400756335

VL - 9789400756342

SP - 561

EP - 565

BT - Functions of Natural Organic Matter in Changing Environment

PB - Springer Netherlands

ER -

Access to Document

10.1007/978-94-007-5634-2_101