Abstract
This chapter considers the effects of humic substances (HS) on solutions of model inorganic pollutants, metal salts - K3[Fe(CN)6] and CuSO4. Bioluminescent system of coupled enzymatic reactions catalyzed by NAD(P)H-FMN oxidoreductase and bacterial luciferase was used as a bioassay to monitor toxicity of salt solutions in the presence and absence of HS. Ability of HS to decrease or increase general toxicity of the solutions was revealed. The rates of redox reaction in bioluminescent assay system were analyzed in the absence and presence of HS. Acceleration of NADH auto-oxidation by HS was supposed to result in toxicity increase. Acceleration of endogenous NADH-dependent redox reactions in the bioluminescent assay system is supposed to be responsible for detoxification of the salt solutions.
| Original language | English |
|---|---|
| Title of host publication | Functions of Natural Organic Matter in Changing Environment |
| Publisher | Springer Netherlands |
| Pages | 561-565 |
| Number of pages | 5 |
| Volume | 9789400756342 |
| ISBN (Electronic) | 9789400756342 |
| ISBN (Print) | 940075633X, 9789400756335 |
| DOIs | |
| Publication status | Published - 1 Aug 2013 |
| Externally published | Yes |
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Keywords
- Bioluminescent assay
- Enzyme reaction rates
- Humic substances
- Metal salts
- Toxicity
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Environmental Science(all)
- Engineering(all)
Cite this
Reaction rates in enzymatic assay system in solutions of metal salts and humic substances. / Tarasova, Anna S.; Kudryasheva, Nadezhda S.
Functions of Natural Organic Matter in Changing Environment. Vol. 9789400756342 Springer Netherlands, 2013. p. 561-565.Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Reaction rates in enzymatic assay system in solutions of metal salts and humic substances
AU - Tarasova, Anna S.
AU - Kudryasheva, Nadezhda S.
PY - 2013/8/1
Y1 - 2013/8/1
N2 - This chapter considers the effects of humic substances (HS) on solutions of model inorganic pollutants, metal salts - K3[Fe(CN)6] and CuSO4. Bioluminescent system of coupled enzymatic reactions catalyzed by NAD(P)H-FMN oxidoreductase and bacterial luciferase was used as a bioassay to monitor toxicity of salt solutions in the presence and absence of HS. Ability of HS to decrease or increase general toxicity of the solutions was revealed. The rates of redox reaction in bioluminescent assay system were analyzed in the absence and presence of HS. Acceleration of NADH auto-oxidation by HS was supposed to result in toxicity increase. Acceleration of endogenous NADH-dependent redox reactions in the bioluminescent assay system is supposed to be responsible for detoxification of the salt solutions.
AB - This chapter considers the effects of humic substances (HS) on solutions of model inorganic pollutants, metal salts - K3[Fe(CN)6] and CuSO4. Bioluminescent system of coupled enzymatic reactions catalyzed by NAD(P)H-FMN oxidoreductase and bacterial luciferase was used as a bioassay to monitor toxicity of salt solutions in the presence and absence of HS. Ability of HS to decrease or increase general toxicity of the solutions was revealed. The rates of redox reaction in bioluminescent assay system were analyzed in the absence and presence of HS. Acceleration of NADH auto-oxidation by HS was supposed to result in toxicity increase. Acceleration of endogenous NADH-dependent redox reactions in the bioluminescent assay system is supposed to be responsible for detoxification of the salt solutions.
KW - Bioluminescent assay
KW - Enzyme reaction rates
KW - Humic substances
KW - Metal salts
KW - Toxicity
UR - http://www.scopus.com/inward/record.url?scp=84932615554&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84932615554&partnerID=8YFLogxK
U2 - 10.1007/978-94-007-5634-2_101
DO - 10.1007/978-94-007-5634-2_101
M3 - Chapter
AN - SCOPUS:84932615554
SN - 940075633X
SN - 9789400756335
VL - 9789400756342
SP - 561
EP - 565
BT - Functions of Natural Organic Matter in Changing Environment
PB - Springer Netherlands
ER -