Reaction rates in enzymatic assay system in solutions of metal salts and humic substances

Anna S. Tarasova, Nadezhda S. Kudryasheva

Research output: Chapter in Book/Report/Conference proceedingChapter


This chapter considers the effects of humic substances (HS) on solutions of model inorganic pollutants, metal salts - K3[Fe(CN)6] and CuSO4. Bioluminescent system of coupled enzymatic reactions catalyzed by NAD(P)H-FMN oxidoreductase and bacterial luciferase was used as a bioassay to monitor toxicity of salt solutions in the presence and absence of HS. Ability of HS to decrease or increase general toxicity of the solutions was revealed. The rates of redox reaction in bioluminescent assay system were analyzed in the absence and presence of HS. Acceleration of NADH auto-oxidation by HS was supposed to result in toxicity increase. Acceleration of endogenous NADH-dependent redox reactions in the bioluminescent assay system is supposed to be responsible for detoxification of the salt solutions.

Original languageEnglish
Title of host publicationFunctions of Natural Organic Matter in Changing Environment
PublisherSpringer Netherlands
Number of pages5
ISBN (Electronic)9789400756342
ISBN (Print)940075633X, 9789400756335
Publication statusPublished - 1 Aug 2013
Externally publishedYes


  • Bioluminescent assay
  • Enzyme reaction rates
  • Humic substances
  • Metal salts
  • Toxicity

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Environmental Science(all)
  • Engineering(all)

Fingerprint Dive into the research topics of 'Reaction rates in enzymatic assay system in solutions of metal salts and humic substances'. Together they form a unique fingerprint.

Cite this