Reaction rates in enzymatic assay system in solutions of metal salts and humic substances

Abstract

This chapter considers the effects of humic substances (HS) on solutions of model inorganic pollutants, metal salts - K₃[Fe(CN)₆] and CuSO₄. Bioluminescent system of coupled enzymatic reactions catalyzed by NAD(P)H-FMN oxidoreductase and bacterial luciferase was used as a bioassay to monitor toxicity of salt solutions in the presence and absence of HS. Ability of HS to decrease or increase general toxicity of the solutions was revealed. The rates of redox reaction in bioluminescent assay system were analyzed in the absence and presence of HS. Acceleration of NADH auto-oxidation by HS was supposed to result in toxicity increase. Acceleration of endogenous NADH-dependent redox reactions in the bioluminescent assay system is supposed to be responsible for detoxification of the salt solutions.

Original language	English
Title of host publication	Functions of Natural Organic Matter in Changing Environment
Publisher	Springer Netherlands
Pages	561-565
Number of pages	5
Volume	9789400756342
ISBN (Electronic)	9789400756342
ISBN (Print)	940075633X, 9789400756335
DOIs	https://doi.org/10.1007/978-94-007-5634-2_101
Publication status	Published - 1 Aug 2013
Externally published	Yes

Keywords

Bioluminescent assay
Enzyme reaction rates
Humic substances
Metal salts
Toxicity

ASJC Scopus subject areas

Agricultural and Biological Sciences(all)
Environmental Science(all)
Engineering(all)

Access to Document

10.1007/978-94-007-5634-2_101

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Tarasova, A. S., & Kudryasheva, N. S. (2013). Reaction rates in enzymatic assay system in solutions of metal salts and humic substances. In Functions of Natural Organic Matter in Changing Environment (Vol. 9789400756342, pp. 561-565). Springer Netherlands. https://doi.org/10.1007/978-94-007-5634-2_101

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abstract = "This chapter considers the effects of humic substances (HS) on solutions of model inorganic pollutants, metal salts - K3[Fe(CN)6] and CuSO4. Bioluminescent system of coupled enzymatic reactions catalyzed by NAD(P)H-FMN oxidoreductase and bacterial luciferase was used as a bioassay to monitor toxicity of salt solutions in the presence and absence of HS. Ability of HS to decrease or increase general toxicity of the solutions was revealed. The rates of redox reaction in bioluminescent assay system were analyzed in the absence and presence of HS. Acceleration of NADH auto-oxidation by HS was supposed to result in toxicity increase. Acceleration of endogenous NADH-dependent redox reactions in the bioluminescent assay system is supposed to be responsible for detoxification of the salt solutions.",

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author = "Tarasova, {Anna S.} and Kudryasheva, {Nadezhda S.}",

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N2 - This chapter considers the effects of humic substances (HS) on solutions of model inorganic pollutants, metal salts - K3[Fe(CN)6] and CuSO4. Bioluminescent system of coupled enzymatic reactions catalyzed by NAD(P)H-FMN oxidoreductase and bacterial luciferase was used as a bioassay to monitor toxicity of salt solutions in the presence and absence of HS. Ability of HS to decrease or increase general toxicity of the solutions was revealed. The rates of redox reaction in bioluminescent assay system were analyzed in the absence and presence of HS. Acceleration of NADH auto-oxidation by HS was supposed to result in toxicity increase. Acceleration of endogenous NADH-dependent redox reactions in the bioluminescent assay system is supposed to be responsible for detoxification of the salt solutions.

AB - This chapter considers the effects of humic substances (HS) on solutions of model inorganic pollutants, metal salts - K3[Fe(CN)6] and CuSO4. Bioluminescent system of coupled enzymatic reactions catalyzed by NAD(P)H-FMN oxidoreductase and bacterial luciferase was used as a bioassay to monitor toxicity of salt solutions in the presence and absence of HS. Ability of HS to decrease or increase general toxicity of the solutions was revealed. The rates of redox reaction in bioluminescent assay system were analyzed in the absence and presence of HS. Acceleration of NADH auto-oxidation by HS was supposed to result in toxicity increase. Acceleration of endogenous NADH-dependent redox reactions in the bioluminescent assay system is supposed to be responsible for detoxification of the salt solutions.

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