Abstract
Nowadays, functionalization of the plasmon-supported nanostructured surface is considered as a powerful tool for tumour cell recognition. In this study, the SERS on a surface plasmon polariton-supported gold grating functionalized with folic acid was used to demonstrate an unpretentious recognition of melanoma-associated fibroblasts. Using cultivation media conditioned by different cells, we were able to detect reproducible differences in the secretome of melanoma-associated and normal control fibroblasts. The homogeneous distribution of plasmon energy along the grating surface was proved to provide excellent SERS signal reproducibility, while, to increase the affinity of (bio)molecules to SERS substrate, folic acid molecules were covalently grafted to the gold gratings. As proof of concept, fibroblasts were cultured in vitro, and culture media from the normal and tumour-associated lines were collected and analysed with our proposed SERS substrates. Identifying individual peaks of the Raman spectra as well as comparing their relative intensities, we showed that the proposed functional SERS platform can recognise the melanoma-associated cells without the need for further statistical spectral evaluation directly. We also demonstrated that the SERS chip created provided a stable SERS signal over a period of 90 days without loss of sensitivity. [Figure not available: see fulltext.].
| Original language | English |
|---|---|
| Pages (from-to) | 3309-3319 |
| Number of pages | 11 |
| Journal | Analytical and Bioanalytical Chemistry |
| Volume | 411 |
| Issue number | 15 |
| DOIs | |
| Publication status | Published - 19 Jun 2019 |
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Keywords
- Cancer
- Melanoma
- Reproducibility of SERS
- SERS sensor
- Surface modification
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
Cite this
Rapid SERS-based recognition of cell secretome on the folic acid-functionalized gold gratings. / Guselnikova, Olga; Dvorankova, Barbara; Kakisheva, Kamila; Kalachyova, Yevgeniya; Postnikov, Pavel; Svorcik, Vaclav; Lyutakov, Oleksiy.
In: Analytical and Bioanalytical Chemistry, Vol. 411, No. 15, 19.06.2019, p. 3309-3319.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Rapid SERS-based recognition of cell secretome on the folic acid-functionalized gold gratings
AU - Guselnikova, Olga
AU - Dvorankova, Barbara
AU - Kakisheva, Kamila
AU - Kalachyova, Yevgeniya
AU - Postnikov, Pavel
AU - Svorcik, Vaclav
AU - Lyutakov, Oleksiy
PY - 2019/6/19
Y1 - 2019/6/19
N2 - Nowadays, functionalization of the plasmon-supported nanostructured surface is considered as a powerful tool for tumour cell recognition. In this study, the SERS on a surface plasmon polariton-supported gold grating functionalized with folic acid was used to demonstrate an unpretentious recognition of melanoma-associated fibroblasts. Using cultivation media conditioned by different cells, we were able to detect reproducible differences in the secretome of melanoma-associated and normal control fibroblasts. The homogeneous distribution of plasmon energy along the grating surface was proved to provide excellent SERS signal reproducibility, while, to increase the affinity of (bio)molecules to SERS substrate, folic acid molecules were covalently grafted to the gold gratings. As proof of concept, fibroblasts were cultured in vitro, and culture media from the normal and tumour-associated lines were collected and analysed with our proposed SERS substrates. Identifying individual peaks of the Raman spectra as well as comparing their relative intensities, we showed that the proposed functional SERS platform can recognise the melanoma-associated cells without the need for further statistical spectral evaluation directly. We also demonstrated that the SERS chip created provided a stable SERS signal over a period of 90 days without loss of sensitivity. [Figure not available: see fulltext.].
AB - Nowadays, functionalization of the plasmon-supported nanostructured surface is considered as a powerful tool for tumour cell recognition. In this study, the SERS on a surface plasmon polariton-supported gold grating functionalized with folic acid was used to demonstrate an unpretentious recognition of melanoma-associated fibroblasts. Using cultivation media conditioned by different cells, we were able to detect reproducible differences in the secretome of melanoma-associated and normal control fibroblasts. The homogeneous distribution of plasmon energy along the grating surface was proved to provide excellent SERS signal reproducibility, while, to increase the affinity of (bio)molecules to SERS substrate, folic acid molecules were covalently grafted to the gold gratings. As proof of concept, fibroblasts were cultured in vitro, and culture media from the normal and tumour-associated lines were collected and analysed with our proposed SERS substrates. Identifying individual peaks of the Raman spectra as well as comparing their relative intensities, we showed that the proposed functional SERS platform can recognise the melanoma-associated cells without the need for further statistical spectral evaluation directly. We also demonstrated that the SERS chip created provided a stable SERS signal over a period of 90 days without loss of sensitivity. [Figure not available: see fulltext.].
KW - Cancer
KW - Melanoma
KW - Reproducibility of SERS
KW - SERS sensor
KW - Surface modification
UR - http://www.scopus.com/inward/record.url?scp=85066499217&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85066499217&partnerID=8YFLogxK
U2 - 10.1007/s00216-019-01801-6
DO - 10.1007/s00216-019-01801-6
M3 - Article
VL - 411
SP - 3309
EP - 3319
JO - Fresenius Zeitschrift fur Analytische Chemie
JF - Fresenius Zeitschrift fur Analytische Chemie
SN - 0016-1152
IS - 15
ER -