Radiolabeled Complex of Antimicrobial Peptides UBI29-41 and UBI18-35 Labeled with 99mTc for Differential Diagnosis of Bone Infection of the Limbs

V. D. Zavadovskaya, M. S. Larkina, E. S. Stasyuk, M. A. Zorkaltsev, V. D. Udodov, V. V. Ivanov, N. M. Shevtsova, A. S. Rogov, A. Ya Lushchyk, A. V. Yantsevich, M. V. Belousov, A. N. Baikov, V. S. Skuridin, A. G. Pershina

Research output: Contribution to journalArticlepeer-review

Abstract

We studied an original radiolabeled complex of antimicrobial peptides UBI29-41 and UBI18-35, ubiquicidin derivatives, for distinguishing between bacterial and aseptic inflammation. For radiolabeling of the peptides with technetium-99m, a bifunctional chelating agent succinimide-1-yl 6-(bis(pyridin-2-ylmethyl)amino)hexanoate was used. The obtained complexes 99mТс-DPAH-UBI29-41 and 99mТс-DPAH-UBI18-35 had radiolabeling yield >80% and radiochemical purity >96%. Accumulation of the complexes in the focus of bacterial inflammation in bone structures and the absence of this complex in the site of aseptic inflammation was confirmed in a rat model of traumatic osteomyelitis by single-photon emission computed tomography.

Original languageEnglish
Pages (from-to)415-419
Number of pages5
JournalBulletin of Experimental Biology and Medicine
Volume170
Issue number4
DOIs
Publication statusPublished - Feb 2021

Keywords

  • osteomyelitis
  • SPECT
  • technetium-99m
  • ubiquicidin

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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