Radio-iodination of monoclonal antibody using potassium [ 125I]-(4-isothiocyanatobenzylammonio)-iodo-decahydro-closo- dodecaborate (Iodo-DABI)

Anna Orlova, Alexander Bruskin, Igor Sivaev, Stefan Sjöberg, Hans Lundqvist, Vladimir Tolmachev

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Background: Negatively-charged polyhedral boron clusters can be easily halogenated with highly stable boron-halogen bonds and are promising in radionuclide diagnostics and cancer therapy. Materials and Methods: The radio-iodination conditions for the closo-dodecaborate anion and for the conjugation of its labeled isothiocyanatobenzylammonio derivative to the monoclonal antibody (mAb) were optimized. Results: The labeling yield was about 90% and the overall conjugation yield was 55-60%. The in vitro stability of the radio-iodinated mAb was good under physiological and non-physiological conditions. The immunoreactivity of the labeled mAb (SK-BR-3 cells) was retained in the one-pot two-step labeling. Conclusion: Negatively-charged polyhedral boron clusters can be used for indirect radio-iodination of mAbs.

Original languageEnglish
Pages (from-to)1217-1223
Number of pages7
JournalAnticancer Research
Volume26
Issue number2 A
Publication statusPublished - Mar 2006
Externally publishedYes

Keywords

  • (4- isothiocyanatobenzylammonio)-iodo-decahydro-closo-dodecaborate
  • Indirect radioiodination
  • Monoclonal antibody

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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