Purification of radiolabeled monoclonal antibodies to angiotensin-converting enzyme significantly improves specificity and efficacy of its targeting into the lung

H. Hiemisch, V. Gavrilyuk, Elena Nikolaevna Atochina, M. Slinkin, V. Torchilin, V. Muzykantov, S. Danilov

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The aim of this study was to improve the labeling/purification procedures for monoclonal antibody (MoAb) to angiotensin-converting enzyme (ACE). MoAb 9B9 was very stable upon iodination at a wide range of iodogen concentrations and incubation times, and was also very stable upon storage, indicating the high technological potential of this MoAb. Radiolabeled MoAb 9B9 was purified by (i) adsorption chromatography on cellulose, (ii) HPLC (gel filtration) and (iii) affinity chromatography on ACE-Sepharose. The best result was obtained with cellulose: specificity of MoAb 9B9 accumulation in the lung increased 2-fold. We conclude that the phenomenon of specific lung accumulation of MoAb 9B9 may serve as an ideal (convenient, cheap and technological) assay system for evaluation of monoclonal antibody modification and labeling.

Original languageEnglish
Pages (from-to)435-441
Number of pages7
JournalNuclear Medicine and Biology
Volume20
Issue number4
DOIs
Publication statusPublished - 1993
Externally publishedYes

Fingerprint

Peptidyl-Dipeptidase A
Monoclonal Antibodies
Lung
Cellulose
Halogenation
Affinity Chromatography
Sepharose
Adsorption
Gel Chromatography
Chromatography
High Pressure Liquid Chromatography

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Medicine
  • Radiology Nuclear Medicine and imaging

Cite this

Purification of radiolabeled monoclonal antibodies to angiotensin-converting enzyme significantly improves specificity and efficacy of its targeting into the lung. / Hiemisch, H.; Gavrilyuk, V.; Atochina, Elena Nikolaevna; Slinkin, M.; Torchilin, V.; Muzykantov, V.; Danilov, S.

In: Nuclear Medicine and Biology, Vol. 20, No. 4, 1993, p. 435-441.

Research output: Contribution to journalArticle

@article{e26667b8fd5f42858806fd5007323cc2,
title = "Purification of radiolabeled monoclonal antibodies to angiotensin-converting enzyme significantly improves specificity and efficacy of its targeting into the lung",
abstract = "The aim of this study was to improve the labeling/purification procedures for monoclonal antibody (MoAb) to angiotensin-converting enzyme (ACE). MoAb 9B9 was very stable upon iodination at a wide range of iodogen concentrations and incubation times, and was also very stable upon storage, indicating the high technological potential of this MoAb. Radiolabeled MoAb 9B9 was purified by (i) adsorption chromatography on cellulose, (ii) HPLC (gel filtration) and (iii) affinity chromatography on ACE-Sepharose. The best result was obtained with cellulose: specificity of MoAb 9B9 accumulation in the lung increased 2-fold. We conclude that the phenomenon of specific lung accumulation of MoAb 9B9 may serve as an ideal (convenient, cheap and technological) assay system for evaluation of monoclonal antibody modification and labeling.",
author = "H. Hiemisch and V. Gavrilyuk and Atochina, {Elena Nikolaevna} and M. Slinkin and V. Torchilin and V. Muzykantov and S. Danilov",
year = "1993",
doi = "10.1016/0969-8051(93)90074-5",
language = "English",
volume = "20",
pages = "435--441",
journal = "Nuclear Medicine and Biology",
issn = "0969-8051",
publisher = "Elsevier Inc.",
number = "4",

}

TY - JOUR

T1 - Purification of radiolabeled monoclonal antibodies to angiotensin-converting enzyme significantly improves specificity and efficacy of its targeting into the lung

AU - Hiemisch, H.

AU - Gavrilyuk, V.

AU - Atochina, Elena Nikolaevna

AU - Slinkin, M.

AU - Torchilin, V.

AU - Muzykantov, V.

AU - Danilov, S.

PY - 1993

Y1 - 1993

N2 - The aim of this study was to improve the labeling/purification procedures for monoclonal antibody (MoAb) to angiotensin-converting enzyme (ACE). MoAb 9B9 was very stable upon iodination at a wide range of iodogen concentrations and incubation times, and was also very stable upon storage, indicating the high technological potential of this MoAb. Radiolabeled MoAb 9B9 was purified by (i) adsorption chromatography on cellulose, (ii) HPLC (gel filtration) and (iii) affinity chromatography on ACE-Sepharose. The best result was obtained with cellulose: specificity of MoAb 9B9 accumulation in the lung increased 2-fold. We conclude that the phenomenon of specific lung accumulation of MoAb 9B9 may serve as an ideal (convenient, cheap and technological) assay system for evaluation of monoclonal antibody modification and labeling.

AB - The aim of this study was to improve the labeling/purification procedures for monoclonal antibody (MoAb) to angiotensin-converting enzyme (ACE). MoAb 9B9 was very stable upon iodination at a wide range of iodogen concentrations and incubation times, and was also very stable upon storage, indicating the high technological potential of this MoAb. Radiolabeled MoAb 9B9 was purified by (i) adsorption chromatography on cellulose, (ii) HPLC (gel filtration) and (iii) affinity chromatography on ACE-Sepharose. The best result was obtained with cellulose: specificity of MoAb 9B9 accumulation in the lung increased 2-fold. We conclude that the phenomenon of specific lung accumulation of MoAb 9B9 may serve as an ideal (convenient, cheap and technological) assay system for evaluation of monoclonal antibody modification and labeling.

UR - http://www.scopus.com/inward/record.url?scp=0027255975&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027255975&partnerID=8YFLogxK

U2 - 10.1016/0969-8051(93)90074-5

DO - 10.1016/0969-8051(93)90074-5

M3 - Article

VL - 20

SP - 435

EP - 441

JO - Nuclear Medicine and Biology

JF - Nuclear Medicine and Biology

SN - 0969-8051

IS - 4

ER -