Pulmonary toxicity of instilled silver nanoparticles

Influence of size, coating and rat strain

Joanna Seiffert, Farhana Hussain, Coen Wiegman, Feng Li, Leo Bey, Warren Baker, Alexandra Porter, Mary P. Ryan, Yan Chang, Andrew Gow, Junfeng Zhang, Jie Zhu, Terry D. Tetley, Kian Fan Chung

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Particle size and surface chemistry are potential determinants of silver nanoparticle (AgNP) respiratory toxicity that may also depend on the lung inflammatory state. We compared the effects of intratracheally-administered AgNPs (20nm and 110nm; polyvinylpyrrolidone (PVP) and citrate-capped; 0.1 mg/Kg) in Brown-Norway (BN) and Sprague-Dawley (SD) rats. In BN rats, there was both a neutrophilic and eosinophilic response, while in SD rats, there was a neutrophilic response at day 1, greatest for the 20nm citrate-capped AgNPs. Eosinophilic cationic protein was increased in bronchoalveolar lavage (BAL) in BN and SD rats on day 1. BAL protein and malondialdehyde levels were increased in BN rats at 1 and 7 days, and BAL KC, CCL11 and IL-13 levels at day 1, with increased expression of CCL11 in lung tissue. Pulmonary resistance increased and compliance decreased at day 1, with persistence at day 7. The 20nm, but not the 110 nm, AgNPs increased bronchial hyperresponsiveness on day 1, which continued at day 7 for the citrate-capped AgNPs only. The 20nm versus the 110 nm size were more proinflammatory in terms of neutrophil influx, but there was little difference between the citrate-capped versus the PVP-capped AgNPs. AgNPs can induce pulmonary eosinophilic and neutrophilic inflammation with bronchial hyperresponsiveness, features characteristic of asthma.

Original languageEnglish
Article numbere0119726
JournalPLoS One
Volume10
Issue number3
DOIs
Publication statusPublished - 6 Mar 2015
Externally publishedYes

Fingerprint

nanosilver
Silver
Citric Acid
citrates
Nanoparticles
coatings
Toxicity
Rats
Bronchoalveolar Lavage
lungs
toxicity
Sprague Dawley Rats
Povidone
Coatings
Lung
Rattus norvegicus
rats
Norway
Interleukin-13
asthma

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Seiffert, J., Hussain, F., Wiegman, C., Li, F., Bey, L., Baker, W., ... Chung, K. F. (2015). Pulmonary toxicity of instilled silver nanoparticles: Influence of size, coating and rat strain. PLoS One, 10(3), [e0119726]. https://doi.org/10.1371/journal.pone.0119726

Pulmonary toxicity of instilled silver nanoparticles : Influence of size, coating and rat strain. / Seiffert, Joanna; Hussain, Farhana; Wiegman, Coen; Li, Feng; Bey, Leo; Baker, Warren; Porter, Alexandra; Ryan, Mary P.; Chang, Yan; Gow, Andrew; Zhang, Junfeng; Zhu, Jie; Tetley, Terry D.; Chung, Kian Fan.

In: PLoS One, Vol. 10, No. 3, e0119726, 06.03.2015.

Research output: Contribution to journalArticle

Seiffert, J, Hussain, F, Wiegman, C, Li, F, Bey, L, Baker, W, Porter, A, Ryan, MP, Chang, Y, Gow, A, Zhang, J, Zhu, J, Tetley, TD & Chung, KF 2015, 'Pulmonary toxicity of instilled silver nanoparticles: Influence of size, coating and rat strain', PLoS One, vol. 10, no. 3, e0119726. https://doi.org/10.1371/journal.pone.0119726
Seiffert, Joanna ; Hussain, Farhana ; Wiegman, Coen ; Li, Feng ; Bey, Leo ; Baker, Warren ; Porter, Alexandra ; Ryan, Mary P. ; Chang, Yan ; Gow, Andrew ; Zhang, Junfeng ; Zhu, Jie ; Tetley, Terry D. ; Chung, Kian Fan. / Pulmonary toxicity of instilled silver nanoparticles : Influence of size, coating and rat strain. In: PLoS One. 2015 ; Vol. 10, No. 3.
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