Described is the production of three-dimensional hydrogel protein microchips on the basis of a new polymerization immobilization technology and retaining of the proteins' biochemical properties is shown. Analysis on microchips was conducted using fluorescent spectroscopy and mass spectrometry techniques. Barstar analysis in the same cellular lysate on different microchips gave identical results. Protein hydrogel microchips are for the first time used for quantitative analysis of recombinant protein expression in cells.
|Number of pages||5|
|Journal||Doklady Akademii Nauk|
|Publication status||Published - 2004|
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