Protein microchips analysis of the expression of recombinant barstar

E. I. Dement'eva, A. Yu Rubina, A. A. Stomakhin, S. M. Ivanov, E. Y. Krejndlin, D. S. Ivanov, D. V. Rodin, S. M. Deev, V. S. Prasolov, A. D. Mirzabekov

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Described is the production of three-dimensional hydrogel protein microchips on the basis of a new polymerization immobilization technology and retaining of the proteins' biochemical properties is shown. Analysis on microchips was conducted using fluorescent spectroscopy and mass spectrometry techniques. Barstar analysis in the same cellular lysate on different microchips gave identical results. Protein hydrogel microchips are for the first time used for quantitative analysis of recombinant protein expression in cells.

Original languageEnglish
Pages (from-to)116-120
Number of pages5
JournalDoklady Akademii Nauk
Volume393
Issue number1
Publication statusPublished - 2004

ASJC Scopus subject areas

  • General

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