Abstract
A bioengineering method for self-assembly of multifunctional superstructures with in-advance programmable properties has been proposed. The method employs two unique proteins, barnase and barstar, to rapidly join the structural components together directly in water solutions. The properties of the superstructures can be designed on demand by linking different agents of various sizes and chemical nature, designated for specific goals. As a proof of concept, colloidally stable trifunctional structures have been assembled by binding together magnetic particles, quantum dots, and antibodies using barnase and barstar. The assembly has demonstrated that the bonds between these proteins are strong enough to hold macroscopic (5 nm-3 μm) particles together. Specific interaction of such superstructures with cancer cells resulted in fluorescent labeling of the cells and their responsiveness to magnetic field. The method can be used to join inorganic moieties, organic particles, and single biomolecules for synergistic use in different applications such as biosensors, photonics, and nanomedicine.
| Original language | English |
|---|---|
| Pages (from-to) | 5827-5832 |
| Number of pages | 6 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 107 |
| Issue number | 13 |
| DOIs | |
| Publication status | Published - 30 Mar 2010 |
| Externally published | Yes |
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Keywords
- Cancer cells
- Fusion proteins
- Magnetic nanoparticles
- Superstructures
- Targeting
ASJC Scopus subject areas
- General
Cite this
Protein-assisted self-assembly of multifunctional nanoparticles. / Nikitin, Maxim P.; Zdobnova, Tatiana A.; Lukash, Sergey V.; Stremovskiy, Oleg A.; Deyev, Sergey M.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, No. 13, 30.03.2010, p. 5827-5832.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Protein-assisted self-assembly of multifunctional nanoparticles
AU - Nikitin, Maxim P.
AU - Zdobnova, Tatiana A.
AU - Lukash, Sergey V.
AU - Stremovskiy, Oleg A.
AU - Deyev, Sergey M.
PY - 2010/3/30
Y1 - 2010/3/30
N2 - A bioengineering method for self-assembly of multifunctional superstructures with in-advance programmable properties has been proposed. The method employs two unique proteins, barnase and barstar, to rapidly join the structural components together directly in water solutions. The properties of the superstructures can be designed on demand by linking different agents of various sizes and chemical nature, designated for specific goals. As a proof of concept, colloidally stable trifunctional structures have been assembled by binding together magnetic particles, quantum dots, and antibodies using barnase and barstar. The assembly has demonstrated that the bonds between these proteins are strong enough to hold macroscopic (5 nm-3 μm) particles together. Specific interaction of such superstructures with cancer cells resulted in fluorescent labeling of the cells and their responsiveness to magnetic field. The method can be used to join inorganic moieties, organic particles, and single biomolecules for synergistic use in different applications such as biosensors, photonics, and nanomedicine.
AB - A bioengineering method for self-assembly of multifunctional superstructures with in-advance programmable properties has been proposed. The method employs two unique proteins, barnase and barstar, to rapidly join the structural components together directly in water solutions. The properties of the superstructures can be designed on demand by linking different agents of various sizes and chemical nature, designated for specific goals. As a proof of concept, colloidally stable trifunctional structures have been assembled by binding together magnetic particles, quantum dots, and antibodies using barnase and barstar. The assembly has demonstrated that the bonds between these proteins are strong enough to hold macroscopic (5 nm-3 μm) particles together. Specific interaction of such superstructures with cancer cells resulted in fluorescent labeling of the cells and their responsiveness to magnetic field. The method can be used to join inorganic moieties, organic particles, and single biomolecules for synergistic use in different applications such as biosensors, photonics, and nanomedicine.
KW - Cancer cells
KW - Fusion proteins
KW - Magnetic nanoparticles
KW - Superstructures
KW - Targeting
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U2 - 10.1073/pnas.1001142107
DO - 10.1073/pnas.1001142107
M3 - Article
VL - 107
SP - 5827
EP - 5832
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 13
ER -