Profiling of 179 miRNA Expression in Blood Plasma of Lung Cancer Patients and Cancer-Free Individuals

Ivan A. Zaporozhchenko, Evgeny S. Morozkin, Anastasia A. Ponomaryova, Elena Y. Rykova, Nadezhda V. Cherdyntseva, Aleksandr A. Zheravin, Oksana A. Pashkovskaya, Evgeny A. Pokushalov, Valentin V. Vlassov, Pavel P. Laktionov

    Research output: Contribution to journalArticle

    9 Citations (Scopus)

    Abstract

    Lung cancer is one of major cancers, and survival of lung cancer patients is dictated by the timely detection and diagnosis. Cell-free circulating miRNAs were proposed as candidate biomarkers for lung cancer. These RNAs are frequently deregulated in lung cancer and can persist in bodily fluids for extended periods of time, shielded from degradation by membrane vesicles and biopolymer complexes. To date, several groups reported the presence of lung tumour-specific subsets of miRNAs in blood. Here we describe the profiling of blood plasma miRNAs in lung cancer patients, healthy individuals and endobronchitis patients using miRCURY LNA miRNA qPCR Serum/Plasma Panel (Exiqon). From 241 ratios differently expressed between cancer patients and healthy individuals 19 miRNAs were selected for verification using the same platform. LASSO-penalized logistic regression model, including 10 miRNA ratios comprised of 14 individual miRNAs discriminated lung cancer patients from both control groups with AUC of 0.979.

    Original languageEnglish
    Article number6348
    JournalScientific Reports
    Volume8
    Issue number1
    DOIs
    Publication statusPublished - 1 Dec 2018

    ASJC Scopus subject areas

    • General

    Fingerprint Dive into the research topics of 'Profiling of 179 miRNA Expression in Blood Plasma of Lung Cancer Patients and Cancer-Free Individuals'. Together they form a unique fingerprint.

  • Cite this

    Zaporozhchenko, I. A., Morozkin, E. S., Ponomaryova, A. A., Rykova, E. Y., Cherdyntseva, N. V., Zheravin, A. A., Pashkovskaya, O. A., Pokushalov, E. A., Vlassov, V. V., & Laktionov, P. P. (2018). Profiling of 179 miRNA Expression in Blood Plasma of Lung Cancer Patients and Cancer-Free Individuals. Scientific Reports, 8(1), [6348]. https://doi.org/10.1038/s41598-018-24769-2