Production of recombinant antibodies in lymphoid and non-lymphoid cells

Sergey M. Deyev, Andre Lieber, Boris V. Radko, Oleg L. Polanovsky

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A recombinant tandem of 'chimeric' mouse/human immunoglobulin (Ig) genes was constructed and inserted into plasmid pGEM 1 under the control of the T7 bacteriophage RNA polymerase promoter. Lymphoid (Sp2/0) and non-lymphoid (CHO) cell lines used for transfection contained in their genomes a semisynthetic gene of T7 RNA polymerase and steadily expressed this enzyme. It was shown for the first time that a stable polycystronic transcription of the Ig gene tandem occurs under the control of a single T7 phage promoter, both in lymphoid and non-lymphoid cells. Synthesis of κ-light and ε-heavy Ig chains and functionally active antibodies was observed in the above-mentioned transfected cell lines. © 1993.
Original languageEnglish
Pages (from-to)111-113
Number of pages3
JournalFEBS Letters
Volume330
Issue number2
DOIs
Publication statusPublished - 13 Sep 1993
Externally publishedYes

Fingerprint

Immunoglobulin Genes
Antibody Formation
Genes
Bacteriophage T7
Immunoglobulin Light Chains
Cell Line
Immunoglobulin Heavy Chains
CHO Cells
Antibodies
Transfection
Immunoglobulins
Plasmids
Genome
Bacteriophages
Enzymes
Transcription
Cells
bacteriophage T7 RNA polymerase

Keywords

  • Antibody engineering
  • CHO cell
  • Gene tandem expression
  • Phage T7 promoter
  • Sp2/0 cell

Cite this

Production of recombinant antibodies in lymphoid and non-lymphoid cells. / Deyev, Sergey M.; Lieber, Andre; Radko, Boris V.; Polanovsky, Oleg L.

In: FEBS Letters, Vol. 330, No. 2, 13.09.1993, p. 111-113.

Research output: Contribution to journalArticle

Deyev, Sergey M. ; Lieber, Andre ; Radko, Boris V. ; Polanovsky, Oleg L. / Production of recombinant antibodies in lymphoid and non-lymphoid cells. In: FEBS Letters. 1993 ; Vol. 330, No. 2. pp. 111-113.
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