Production of recombinant antibodies in lymphoid and non-lymphoid cells

Sergey M. Deyev, Andre Lieber, Boris V. Radko, Oleg L. Polanovsky

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A recombinant tandem of 'chimeric' mouse/human immunoglobulin (Ig) genes was constructed and inserted into plasmid pGEM 1 under the control of the T7 bacteriophage RNA polymerase promoter. Lymphoid (Sp2/0) and non-lymphoid (CHO) cell lines used for transfection contained in their genomes a semisynthetic gene of T7 RNA polymerase and steadily expressed this enzyme. It was shown for the first time that a stable polycystronic transcription of the Ig gene tandem occurs under the control of a single T7 phage promoter, both in lymphoid and non-lymphoid cells. Synthesis of κ-light and ε-heavy Ig chains and functionally active antibodies was observed in the above-mentioned transfected cell lines. © 1993.
Original languageEnglish
Pages (from-to)111-113
Number of pages3
JournalFEBS Letters
Volume330
Issue number2
DOIs
Publication statusPublished - 13 Sep 1993
Externally publishedYes

Keywords

  • Antibody engineering
  • CHO cell
  • Gene tandem expression
  • Phage T7 promoter
  • Sp2/0 cell

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