Production and functional characteristics of the recombinant targeted toxin based on the HER2-specific non-immunoglobulin scaffold

E. A. Sokolova, A. A. Schulga, O. A. Stremovskiy, I. V. Balalaeva, G. M. Proshkina, S. M. Deyev

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Designing of new high-affinity protein compounds that can selectively destroy human cancer cells is a priority research area in biomedicine. In this work we report on the production procedure and describe functional characteristics of genetically encoded targeted toxin DARPin-PE40 for selective destruction of HER2-positive human cancer cells. HER2-specific non-immunoglobulin scaffold protein with ankyrin repeat was used as a targeting moiety. As a cytotoxic module, we used a fragment of the Pseudomonas exotoxin A. Using cytoplasmic expression in a bacterial system, we produced a monomelic protein with the yield of 180 mg per 1 L of culture medium. It was shown that targeted recombinant toxin possesses a considerable cytotoxic activity in vitro against HER2-positive cells (IC50 2 pM).

Original languageEnglish
Pages (from-to)429-434
Number of pages6
JournalBiologicheskie Membrany
Volume33
Issue number6
DOIs
Publication statusPublished - 2016

Keywords

  • HER2 receptor
  • Non-immunoglobulin module darpin
  • Pseudomonas aeruginosa exotoxin A
  • Recombinant targeted toxin
  • Targeted therapy

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Production and functional characteristics of the recombinant targeted toxin based on the HER2-specific non-immunoglobulin scaffold'. Together they form a unique fingerprint.

  • Cite this