Preserved cardiac mitochondrial function and reduced ischaemia/reperfusion injury afforded by chronic continuous hypoxia: Role of opioid receptors

Leonid N. Maslov, Natalia V. Naryzhnaya, Ekaterina S. Prokudina, Frantisek Kolar, Alexander S. Gorbunov, Yi Zhang, Hongxin Wang, Sergey Yu Tsibulnikov, Alla G. Portnichenko, Tatiana V. Lasukova, Yury B. Lishmanov

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Chronic continuous normobaric hypoxia (CNH) increases cardiac tolerance to acute ischaemia/reperfusion injury. The objective of this study was to find out whether the cardioprotective effect of CNH mediated by opioid receptors is associated with preservation of mitochondrial function. Rats were adapted to CNH (12% oxygen) for 3 weeks. Isolated perfused hearts were subjected to 45 min of global ischaemia and 30 min of reperfusion; subgroups were pretreated with non-selective opioid receptor antagonist naloxone (300 nmol/L) for 10 min. Cardiac contractile function, creatine kinase activity in coronary effluent, mitochondrial respiration rate, and calcium retention capacity were assessed. Adaptation to CNH decreased myocardial creatine kinase release during reperfusion and improved the post-ischaemic recovery of contractile function, mitochondrial state 3 and uncoupled respiration rates, and calcium retention capacity compared to the normoxic group. These protective effects were completely abolished by naloxone. The contractile recovery positively correlated with state 3 respiration and calcium retention capacity. The results suggest that the preserved mitochondrial function contributes to the protected cardiac phenotype afforded by adaptation to CNH and point to an important role of opioid receptor activation.

Original languageEnglish
Pages (from-to)496-501
Number of pages6
JournalClinical and Experimental Pharmacology and Physiology
Volume42
Issue number5
DOIs
Publication statusPublished - 1 May 2015

Fingerprint

Opioid Receptors
Reperfusion Injury
Respiratory Rate
Naloxone
Calcium
Reperfusion
MB Form Creatine Kinase
Narcotic Antagonists
Recovery of Function
Creatine Kinase
Respiration
Ischemia
Hypoxia
Oxygen
Phenotype

Keywords

  • Cardioprotection
  • Chronic hypoxia
  • Ischaemia/reperfusion
  • Mitochondrial function
  • Opioid receptors

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)
  • Pharmacology

Cite this

Preserved cardiac mitochondrial function and reduced ischaemia/reperfusion injury afforded by chronic continuous hypoxia : Role of opioid receptors. / Maslov, Leonid N.; Naryzhnaya, Natalia V.; Prokudina, Ekaterina S.; Kolar, Frantisek; Gorbunov, Alexander S.; Zhang, Yi; Wang, Hongxin; Tsibulnikov, Sergey Yu; Portnichenko, Alla G.; Lasukova, Tatiana V.; Lishmanov, Yury B.

In: Clinical and Experimental Pharmacology and Physiology, Vol. 42, No. 5, 01.05.2015, p. 496-501.

Research output: Contribution to journalArticle

Maslov, LN, Naryzhnaya, NV, Prokudina, ES, Kolar, F, Gorbunov, AS, Zhang, Y, Wang, H, Tsibulnikov, SY, Portnichenko, AG, Lasukova, TV & Lishmanov, YB 2015, 'Preserved cardiac mitochondrial function and reduced ischaemia/reperfusion injury afforded by chronic continuous hypoxia: Role of opioid receptors', Clinical and Experimental Pharmacology and Physiology, vol. 42, no. 5, pp. 496-501. https://doi.org/10.1111/1440-1681.12383
Maslov, Leonid N. ; Naryzhnaya, Natalia V. ; Prokudina, Ekaterina S. ; Kolar, Frantisek ; Gorbunov, Alexander S. ; Zhang, Yi ; Wang, Hongxin ; Tsibulnikov, Sergey Yu ; Portnichenko, Alla G. ; Lasukova, Tatiana V. ; Lishmanov, Yury B. / Preserved cardiac mitochondrial function and reduced ischaemia/reperfusion injury afforded by chronic continuous hypoxia : Role of opioid receptors. In: Clinical and Experimental Pharmacology and Physiology. 2015 ; Vol. 42, No. 5. pp. 496-501.
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