Preparation of single-stranded antibodies to tick-borne encephalitis virus surface glycoprotein E

N. V. Tikunova, G. N. Nikolenko, Ye V. Protopopova, A. T. Kotelkin, V. B. Loktev, P. A. Belavin, N. A. Netesova, S. M. Deev, A. A. Ilyichev

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

A single-stranded antibody gene scFv was designed on the base of cDNA fragments of genes coding for variable domains of heavy and light chains of MAb E6B to tick-borne encephalitis glycoprotein E. High production of stable soluble scFv was reproduced in Escherichia coli cells. Recombinant antibodies bound to antiidiotypical antibodies to initial MAb E6B and to recombinant virus protein E. Competitive analysis showed that single-stranded antibodies inhibited reaction between MAb E6B and protein E. These results confirm the formation of scFv with the original antigen-binding specificity towards tick-borne encephalitis virus glycoprotein E.

Original languageEnglish
Pages (from-to)12-15
Number of pages4
JournalVoprosy Virusologii
Volume44
Issue number1
Publication statusPublished - 1999

Keywords

  • Characteristics
  • Preparation
  • Recombinant antibodies
  • Surface glycoprotein E
  • Tick-borne encephalitis virus

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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