Preparation of Conjugates for Affibody-Based PNA-Mediated Pretargeting

Mohamed Altai, Anzhelika Vorobyeva, Vladimir Tolmachev, Amelie Eriksson Karlström, Kristina Westerlund

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Affibody molecules are small engineered scaffold proteins suitable for in vivo tumor targeting. Radionuclide molecular imaging using directly radiolabelled affibody molecules provides excellent imaging. However, affibody molecules have a high renal reabsorption, which complicates their use for radionuclide therapy. The high renal reabsorption is a common problem for the use of engineered scaffold proteins for radionuclide therapy. Affibody-based PNA-mediated pretargeting reduces dramatically the absorbed dose to the kidneys and makes affibody-based radionuclide therapy possible. This methodology might, hopefully, solve the problem of high renal reabsorption for radionuclide therapy mediated by other engineered scaffold proteins.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages283-304
Number of pages22
DOIs
Publication statusPublished - 2020
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume2105
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • In
  • Lu
  • Co
  • Ga
  • Affibody molecules
  • Peptide synthesis
  • PNA
  • Pretargeting
  • Scaffold protein
  • Sortase A

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Preparation of Conjugates for Affibody-Based PNA-Mediated Pretargeting'. Together they form a unique fingerprint.

Cite this