TY - JOUR
T1 - Pre-osteoblastic cell response on three-dimensional, organic-inorganic hybrid material scaffolds for bone tissue engineering
AU - Terzaki, Konstantina
AU - Kissamitaki, Maria
AU - Skarmoutsou, Amalia
AU - Fotakis, Costas
AU - Charitidis, Costas A.
AU - Farsari, Maria
AU - Vamvakaki, Maria
AU - Chatzinikolaidou, Maria
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2013/8
Y1 - 2013/8
N2 - Engineering artificial scaffolds that enhance cell adhesion and growth in three dimensions is essential to successful bone tissue engineering. However, the fabrication of three-dimensional (3D) tissue scaffolds exhibiting complex micro- and nano-features still remains a challenge. Few materials can be structured in three dimensions, and even those have not been characterized for their mechanical and biological properties. In this study, we investigate the suitability of three novel materials of different chemical compositions in bone tissue regeneration: a hybrid material consisting of methacryloxypropyl trimethoxysilane and zirconium propoxide, a hybrid organic-inorganic material of the above containing 50 mole% 2-(dimethylamino)ethyl methacrylate (DMAEMA) and a pure organic material based on polyDMAEMA. More specifically, we study the mechanical properties of the aforementioned materials and evaluate the biological response of pre-osteoblastic cells on them. We also highlight the use of a 3D scaffolding technology, Direct femtosecond Laser Writing (DLW), to fabricate complex structures. Our results show that, while all three investigated materials could potentially be used as biomaterials in tissue engineering, the 50% DMAEMA composite exhibits the best mechanical properties for structure fabrication with DLW and strong biological response.
AB - Engineering artificial scaffolds that enhance cell adhesion and growth in three dimensions is essential to successful bone tissue engineering. However, the fabrication of three-dimensional (3D) tissue scaffolds exhibiting complex micro- and nano-features still remains a challenge. Few materials can be structured in three dimensions, and even those have not been characterized for their mechanical and biological properties. In this study, we investigate the suitability of three novel materials of different chemical compositions in bone tissue regeneration: a hybrid material consisting of methacryloxypropyl trimethoxysilane and zirconium propoxide, a hybrid organic-inorganic material of the above containing 50 mole% 2-(dimethylamino)ethyl methacrylate (DMAEMA) and a pure organic material based on polyDMAEMA. More specifically, we study the mechanical properties of the aforementioned materials and evaluate the biological response of pre-osteoblastic cells on them. We also highlight the use of a 3D scaffolding technology, Direct femtosecond Laser Writing (DLW), to fabricate complex structures. Our results show that, while all three investigated materials could potentially be used as biomaterials in tissue engineering, the 50% DMAEMA composite exhibits the best mechanical properties for structure fabrication with DLW and strong biological response.
KW - cell adhesion
KW - hybrid material
KW - MC3T3-E1 pre-osteoblasts
KW - nanomechanical characterization
KW - three-dimensional scaffold fabrication
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U2 - 10.1002/jbm.a.34516
DO - 10.1002/jbm.a.34516
M3 - Article
C2 - 23355483
AN - SCOPUS:84879500576
VL - 101 A
SP - 2283
EP - 2294
JO - Journal of Biomedical Materials Research - Part A
JF - Journal of Biomedical Materials Research - Part A
SN - 1549-3296
IS - 8
ER -