Polysaccha-rides of three saussurea dc species (S. Controversa, S. Salicifolia, S. Frolovii): Isolation, characterization and influence on no-producing properties of macrophages

Abstract

Polysaccharides PS1 and PS2 with a yield 1-2% were obtained from the above-ground part of three species of Saussurea: S. controversa DC., S. salicifolia L. and S. frolovii Ledeb. sequential extraction with water at 25 and 70 °C. PS1 free from protein impurities. A protein that is not removed by the Sevag method is co-extracted with PS2. All polysaccharides contain residues of uronic acids, the highest content found in PS₁ and PS₂ from S. contrversa. The molecular weights (Mw) of PS1 from S. contro-versa, S. salicifolia and S. frolovii were 448.13, 158.49, 64.03 kDa and PS₂ - 101.82, 94.60, 225.42 kDa, respectively. Interspe-cific differences in the monosaccharide composition of polysaccharides were revealed. Galactose (Gal) and Arabinose (Ara) residues are major, and Rhamnose (Rha), Xylose (Xyl) and Mannose (Man) residues are minor components of the carbohydrate chains isolated by PS. PS₁ S. salicifolia and S. frolovii and PS₂ S. salicifolia do not contain endotoxins impurities and have a NO-activating effect on antigen-presenting cells (macrophages), significantly exceeding the effect of muramyl dipeptide.

Original language	English
Pages (from-to)	77-85
Number of pages	9
Journal	Khimiya Rastitel'nogo Syr'ya
Issue number	4
DOIs	https://doi.org/10.14258/jcprm.2019045483
Publication status	Published - 1 Dec 2019

Keywords

Asteraceae
Endotoxin
Mac-rophages
Molecular weight
Monosaccharide composition
Nitric oxide
Polysaccharides
Saussurea DC

ASJC Scopus subject areas

Biomaterials
Ecology, Evolution, Behavior and Systematics
Ecology
Plant Science
Organic Chemistry

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Reshetov, Y. Y., Ligachova, A. A., Avdeyeva, Y. Y., Danilets, M. G., Golovchenko, V. V., Trofimova, Y. S., Gulina, Y. I., Sherstoboyev, Y. Y., Gur'Yev, A. M., Rovkina, K. I., Krivoshchekov, S. V., & Belousov, M. V. (2019). Polysaccha-rides of three saussurea dc species (S. Controversa, S. Salicifolia, S. Frolovii): Isolation, characterization and influence on no-producing properties of macrophages. Khimiya Rastitel'nogo Syr'ya, (4), 77-85. https://doi.org/10.14258/jcprm.2019045483

Polysaccha-rides of three saussurea dc species (S. Controversa, S. Salicifolia, S. Frolovii) : Isolation, characterization and influence on no-producing properties of macrophages. / Reshetov, Ya Ye; Ligachova, A. A.; Avdeyeva, Ye Yu; Danilets, M. G.; Golovchenko, V. V.; Trofimova, Ye S.; Gulina, Ye I.; Sherstoboyev, Ye Yu; Gur'Yev, A. M.; Rovkina, K. I.; Krivoshchekov, S. V.; Belousov, M. V.

In: Khimiya Rastitel'nogo Syr'ya, No. 4, 01.12.2019, p. 77-85.

Research output: Contribution to journal › Article › peer-review

Reshetov, YY, Ligachova, AA, Avdeyeva, YY, Danilets, MG, Golovchenko, VV, Trofimova, YS, Gulina, YI, Sherstoboyev, YY, Gur'Yev, AM, Rovkina, KI, Krivoshchekov, SV & Belousov, MV 2019, 'Polysaccha-rides of three saussurea dc species (S. Controversa, S. Salicifolia, S. Frolovii): Isolation, characterization and influence on no-producing properties of macrophages', Khimiya Rastitel'nogo Syr'ya, no. 4, pp. 77-85. https://doi.org/10.14258/jcprm.2019045483

Reshetov, Ya Ye ; Ligachova, A. A. ; Avdeyeva, Ye Yu ; Danilets, M. G. ; Golovchenko, V. V. ; Trofimova, Ye S. ; Gulina, Ye I. ; Sherstoboyev, Ye Yu ; Gur'Yev, A. M. ; Rovkina, K. I. ; Krivoshchekov, S. V. ; Belousov, M. V. / Polysaccha-rides of three saussurea dc species (S. Controversa, S. Salicifolia, S. Frolovii) : Isolation, characterization and influence on no-producing properties of macrophages. In: Khimiya Rastitel'nogo Syr'ya. 2019 ; No. 4. pp. 77-85.

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abstract = "Polysaccharides PS1 and PS2 with a yield 1-2% were obtained from the above-ground part of three species of Saussurea: S. controversa DC., S. salicifolia L. and S. frolovii Ledeb. sequential extraction with water at 25 and 70 °C. PS1 free from protein impurities. A protein that is not removed by the Sevag method is co-extracted with PS2. All polysaccharides contain residues of uronic acids, the highest content found in PS1 and PS2 from S. contrversa. The molecular weights (Mw) of PS1 from S. contro-versa, S. salicifolia and S. frolovii were 448.13, 158.49, 64.03 kDa and PS2 - 101.82, 94.60, 225.42 kDa, respectively. Interspe-cific differences in the monosaccharide composition of polysaccharides were revealed. Galactose (Gal) and Arabinose (Ara) residues are major, and Rhamnose (Rha), Xylose (Xyl) and Mannose (Man) residues are minor components of the carbohydrate chains isolated by PS. PS1 S. salicifolia and S. frolovii and PS2 S. salicifolia do not contain endotoxins impurities and have a NO-activating effect on antigen-presenting cells (macrophages), significantly exceeding the effect of muramyl dipeptide.",

keywords = "Asteraceae, Endotoxin, Mac-rophages, Molecular weight, Monosaccharide composition, Nitric oxide, Polysaccharides, Saussurea DC",

author = "Reshetov, {Ya Ye} and Ligachova, {A. A.} and Avdeyeva, {Ye Yu} and Danilets, {M. G.} and Golovchenko, {V. V.} and Trofimova, {Ye S.} and Gulina, {Ye I.} and Sherstoboyev, {Ye Yu} and Gur'Yev, {A. M.} and Rovkina, {K. I.} and Krivoshchekov, {S. V.} and Belousov, {M. V.}",

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T2 - Isolation, characterization and influence on no-producing properties of macrophages

AU - Reshetov, Ya Ye

AU - Ligachova, A. A.

AU - Avdeyeva, Ye Yu

AU - Danilets, M. G.

AU - Golovchenko, V. V.

AU - Trofimova, Ye S.

AU - Gulina, Ye I.

AU - Sherstoboyev, Ye Yu

AU - Gur'Yev, A. M.

AU - Rovkina, K. I.

AU - Krivoshchekov, S. V.

AU - Belousov, M. V.

PY - 2019/12/1

Y1 - 2019/12/1

N2 - Polysaccharides PS1 and PS2 with a yield 1-2% were obtained from the above-ground part of three species of Saussurea: S. controversa DC., S. salicifolia L. and S. frolovii Ledeb. sequential extraction with water at 25 and 70 °C. PS1 free from protein impurities. A protein that is not removed by the Sevag method is co-extracted with PS2. All polysaccharides contain residues of uronic acids, the highest content found in PS1 and PS2 from S. contrversa. The molecular weights (Mw) of PS1 from S. contro-versa, S. salicifolia and S. frolovii were 448.13, 158.49, 64.03 kDa and PS2 - 101.82, 94.60, 225.42 kDa, respectively. Interspe-cific differences in the monosaccharide composition of polysaccharides were revealed. Galactose (Gal) and Arabinose (Ara) residues are major, and Rhamnose (Rha), Xylose (Xyl) and Mannose (Man) residues are minor components of the carbohydrate chains isolated by PS. PS1 S. salicifolia and S. frolovii and PS2 S. salicifolia do not contain endotoxins impurities and have a NO-activating effect on antigen-presenting cells (macrophages), significantly exceeding the effect of muramyl dipeptide.

AB - Polysaccharides PS1 and PS2 with a yield 1-2% were obtained from the above-ground part of three species of Saussurea: S. controversa DC., S. salicifolia L. and S. frolovii Ledeb. sequential extraction with water at 25 and 70 °C. PS1 free from protein impurities. A protein that is not removed by the Sevag method is co-extracted with PS2. All polysaccharides contain residues of uronic acids, the highest content found in PS1 and PS2 from S. contrversa. The molecular weights (Mw) of PS1 from S. contro-versa, S. salicifolia and S. frolovii were 448.13, 158.49, 64.03 kDa and PS2 - 101.82, 94.60, 225.42 kDa, respectively. Interspe-cific differences in the monosaccharide composition of polysaccharides were revealed. Galactose (Gal) and Arabinose (Ara) residues are major, and Rhamnose (Rha), Xylose (Xyl) and Mannose (Man) residues are minor components of the carbohydrate chains isolated by PS. PS1 S. salicifolia and S. frolovii and PS2 S. salicifolia do not contain endotoxins impurities and have a NO-activating effect on antigen-presenting cells (macrophages), significantly exceeding the effect of muramyl dipeptide.

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