Polarographic and voltammetric determination of genotoxic 2-aminofluoren-9-one at mercury electrodes

Electrochemical behavior of genotoxic 2-aminofluoren-9-one (2-AFN) was investigated by DC tast polarography (DCTP) and differential pulse polarography (DPP), both at a classical dropping mercury electrode (DME), and by DC voltammetry (DCV), differential pulse voltammetry (DPV), and adsorptive stripping differential pulse voltammetry (AdSDPV), all at a miniaturized hanging mercury drop minielectrode (HMDmE), in buffered aqueousmethanolic solutions. Optimum conditions were found for the determination of 2-AFN by DCTP at DME in the concentration range from 1 × 10 ^-6 to 1 × 10 ^-4 mol l ^-1 (with a limit of quantification (L _Q) of 5 × 10 ^-7 mol l ^-1), by DPP at DME (from 1 × 10 ^-7 to 1 × 10 ^-4 mol l ^-1; L _Q ≈ 1 × 10 ^-7 mol l ^-1), by DCV and DPV at HMDmE (both from 1 × 10 ^-7 to 1 × 10 ^-4 mol l ^-1; L _Qs ≈ 2 × 10 ^-7 and 1 × 10 ^-7 mol l ^-1 for DCV and DPV, respectively), and by AdSDPV at HMDmE (from 2 × 10 ^-9 to 1 × 10 ^-7 mol l ^-1; L _Q ≈ 4 × 10 ^-9 mol l ^-1). Practical applicability of the developed methods was verified on the direct determination of 2-AFN in model samples of drinking and river water in nanomolar to micromolar concentrations.