Point-wise laser effect on NIH/3T3 cells impregnated with photosensitizer-loaded porous calcium carbonate microparticles

Yulia Svenskaya, Dmitry Gorin, Bogdan Parakhonskiy, Gleb Sukhorukov

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Citations (Scopus)

Abstract

The development of novel carriers for the targeted photosensitizer delivery is a significant problem in a modern photodynamic therapy. Such a nanomedicine research is aimed to reduce the sides effects connected with insufficient selectivity of action for the applied dye. Porous calcium carbonate (CaCO3) particles were proposed as matrices for the photosensitizer Photosens encapsulation. Cellular uptake and phototoxicity of the carriers were investigated using NIH/3T3 cells. The local concentration of the dye inside of the cell was increased by the usage of Photosens-loaded CaCO3 particles in comparison with the free Photosens solution, due to the fact, that the particles trapped by cells provided the dye localization inside. Cellular experiments demonstrated high penetration of containers into the cells within 24h. The point-wise laser (670 nm, 3 mW, 5 min) effect on cells was investigated by confocal laser scanning microscopy. Significant phototoxic effect was shown for Photosens-loaded CaCO3 particles allowing controlled consequential cell destroying by the laser. The demonstrated effect hold out the prospect for the application of proposed system in anticancer PDT.

Original languageEnglish
Title of host publicationIEEE-NANO 2015 - 15th International Conference on Nanotechnology
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages1513-1516
Number of pages4
ISBN (Electronic)9781467381550
DOIs
Publication statusPublished - 20 Jan 2016
Externally publishedYes
Event15th IEEE International Conference on Nanotechnology, IEEE-NANO 2015 - Rome, Italy
Duration: 27 Jul 201530 Jul 2015

Conference

Conference15th IEEE International Conference on Nanotechnology, IEEE-NANO 2015
CountryItaly
CityRome
Period27.7.1530.7.15

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Keywords

  • calcium carbonate
  • photodynamic therapy
  • photosensitizer
  • point-wise laser effect
  • submicrone particles
  • vaterite

ASJC Scopus subject areas

  • Process Chemistry and Technology
  • Electrical and Electronic Engineering
  • Ceramics and Composites
  • Electronic, Optical and Magnetic Materials
  • Surfaces, Coatings and Films

Cite this

Svenskaya, Y., Gorin, D., Parakhonskiy, B., & Sukhorukov, G. (2016). Point-wise laser effect on NIH/3T3 cells impregnated with photosensitizer-loaded porous calcium carbonate microparticles. In IEEE-NANO 2015 - 15th International Conference on Nanotechnology (pp. 1513-1516). [7388931] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/NANO.2015.7388931