Abstract
The influence of platinum derivatives, cisplatin (cis-diamminedichloroplatinum) and imidazolplatin (cis-diimidazoledichloroplatinum) on the activity of rat liver microsomal NADPH-oxidoreductases was investigated in vitro using spectrophotometrical and chemiluminometrical methods. In the range of concentrations from 1 to 12 μM cisplatin inhibited NADPH-cytochrome c-reductase activity and NADPH/lucigenin-dependent chemiluminescence of microsomes. The 3 μM cisplatin decreased the NADPH-dependent reductase activity by 50%. At concentrations less than 2,5 mM sanazole (drug AK-2123) did not prevent the inhibiting influence of cisplatin on microsomal NADPH-dependent reductases. Imidazolplatin insignificantly inhibited NADPH-reductases. It is concluded that negligible inhibiting effect of imidazolplatin on microsomal NADPH-oxidoreductase allows us to consider, this platinum derivative as a promising compound for further experimental trials as anticancer drug with low toxic action on the normal tissues of an organism.
| Original language | English |
|---|---|
| Pages (from-to) | 380-381 |
| Number of pages | 2 |
| Journal | Voprosy Meditsinskoj Khimii |
| Volume | 47 |
| Issue number | 4 |
| Publication status | Published - 2001 |
| Externally published | Yes |
Keywords
- Chemiluminescence
- Cisplatin
- Microsomes
- NAD(P)H-dependent reductases
- Platinum derivatives
ASJC Scopus subject areas
- Biochemistry
- Biotechnology
- Clinical Biochemistry
- Molecular Biology
- Pharmacology