Platinum derivative effect on activity of microsomal NADPH-oxidoreductases

I. A. Shchepetkin, V. M. Plotnikov, V. T. Kagiya

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

The influence of platinum derivatives, cisplatin (cis-diamminedichloroplatinum) and imidazolplatin (cis-diimidazoledichloroplatinum) on the activity of rat liver microsomal NADPH-oxidoreductases was investigated in vitro using spectrophotometrical and chemiluminometrical methods. In the range of concentrations from 1 to 12 μM cisplatin inhibited NADPH-cytochrome c-reductase activity and NADPH/lucigenin-dependent chemiluminescence of microsomes. The 3 μM cisplatin decreased the NADPH-dependent reductase activity by 50%. At concentrations less than 2,5 mM sanazole (drug AK-2123) did not prevent the inhibiting influence of cisplatin on microsomal NADPH-dependent reductases. Imidazolplatin insignificantly inhibited NADPH-reductases. It is concluded that negligible inhibiting effect of imidazolplatin on microsomal NADPH-oxidoreductase allows us to consider, this platinum derivative as a promising compound for further experimental trials as anticancer drug with low toxic action on the normal tissues of an organism.

Original languageEnglish
Pages (from-to)380-381
Number of pages2
JournalVoprosy Meditsinskoj Khimii
Volume47
Issue number4
Publication statusPublished - 2001
Externally publishedYes

Keywords

  • Chemiluminescence
  • Cisplatin
  • Microsomes
  • NAD(P)H-dependent reductases
  • Platinum derivatives

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Clinical Biochemistry
  • Molecular Biology
  • Pharmacology

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