Platinum derivative effect on activity of microsomal NADPH-oxidoreductases

I. A. Shchepetkin, V. M. Plotnikov, V. T. Kagiya

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The influence of platinum derivatives, cisplatin (cis-diamminedichloroplatinum) and imidazolplatin (cis-diimidazoledichloroplatinum) on the activity of rat liver microsomal NADPH-oxidoreductases was investigated in vitro using spectrophotometrical and chemiluminometrical methods. In the range of concentrations from 1 to 12 μM cisplatin inhibited NADPH-cytochrome c-reductase activity and NADPH/lucigenin-dependent chemiluminescence of microsomes. The 3 μM cisplatin decreased the NADPH-dependent reductase activity by 50%. At concentrations less than 2,5 mM sanazole (drug AK-2123) did not prevent the inhibiting influence of cisplatin on microsomal NADPH-dependent reductases. Imidazolplatin insignificantly inhibited NADPH-reductases. It is concluded that negligible inhibiting effect of imidazolplatin on microsomal NADPH-oxidoreductase allows us to consider, this platinum derivative as a promising compound for further experimental trials as anticancer drug with low toxic action on the normal tissues of an organism.

Original languageEnglish
Pages (from-to)380-381
Number of pages2
JournalVoprosy Meditsinskoj Khimii
Volume47
Issue number4
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Platinum
NADP
Oxidoreductases
Derivatives
Cisplatin
Toxic Actions
NADPH-Ferrihemoprotein Reductase
Chemiluminescence
Microsomes
Luminescence
Pharmaceutical Preparations
Liver
Rats
Tissue
diimidazoldichloroplatinum

Keywords

  • Chemiluminescence
  • Cisplatin
  • Microsomes
  • NAD(P)H-dependent reductases
  • Platinum derivatives

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Clinical Biochemistry
  • Molecular Biology
  • Pharmacology

Cite this

Platinum derivative effect on activity of microsomal NADPH-oxidoreductases. / Shchepetkin, I. A.; Plotnikov, V. M.; Kagiya, V. T.

In: Voprosy Meditsinskoj Khimii, Vol. 47, No. 4, 2001, p. 380-381.

Research output: Contribution to journalArticle

Shchepetkin, I. A. ; Plotnikov, V. M. ; Kagiya, V. T. / Platinum derivative effect on activity of microsomal NADPH-oxidoreductases. In: Voprosy Meditsinskoj Khimii. 2001 ; Vol. 47, No. 4. pp. 380-381.
@article{85c97739e7d84fe99de1a2e89ed479b4,
title = "Platinum derivative effect on activity of microsomal NADPH-oxidoreductases",
abstract = "The influence of platinum derivatives, cisplatin (cis-diamminedichloroplatinum) and imidazolplatin (cis-diimidazoledichloroplatinum) on the activity of rat liver microsomal NADPH-oxidoreductases was investigated in vitro using spectrophotometrical and chemiluminometrical methods. In the range of concentrations from 1 to 12 μM cisplatin inhibited NADPH-cytochrome c-reductase activity and NADPH/lucigenin-dependent chemiluminescence of microsomes. The 3 μM cisplatin decreased the NADPH-dependent reductase activity by 50{\%}. At concentrations less than 2,5 mM sanazole (drug AK-2123) did not prevent the inhibiting influence of cisplatin on microsomal NADPH-dependent reductases. Imidazolplatin insignificantly inhibited NADPH-reductases. It is concluded that negligible inhibiting effect of imidazolplatin on microsomal NADPH-oxidoreductase allows us to consider, this platinum derivative as a promising compound for further experimental trials as anticancer drug with low toxic action on the normal tissues of an organism.",
keywords = "Chemiluminescence, Cisplatin, Microsomes, NAD(P)H-dependent reductases, Platinum derivatives",
author = "Shchepetkin, {I. A.} and Plotnikov, {V. M.} and Kagiya, {V. T.}",
year = "2001",
language = "English",
volume = "47",
pages = "380--381",
journal = "Voprosy Meditsinskoj Khimii",
issn = "0042-8809",
publisher = "Russian Academy of Medical Sciences",
number = "4",

}

TY - JOUR

T1 - Platinum derivative effect on activity of microsomal NADPH-oxidoreductases

AU - Shchepetkin, I. A.

AU - Plotnikov, V. M.

AU - Kagiya, V. T.

PY - 2001

Y1 - 2001

N2 - The influence of platinum derivatives, cisplatin (cis-diamminedichloroplatinum) and imidazolplatin (cis-diimidazoledichloroplatinum) on the activity of rat liver microsomal NADPH-oxidoreductases was investigated in vitro using spectrophotometrical and chemiluminometrical methods. In the range of concentrations from 1 to 12 μM cisplatin inhibited NADPH-cytochrome c-reductase activity and NADPH/lucigenin-dependent chemiluminescence of microsomes. The 3 μM cisplatin decreased the NADPH-dependent reductase activity by 50%. At concentrations less than 2,5 mM sanazole (drug AK-2123) did not prevent the inhibiting influence of cisplatin on microsomal NADPH-dependent reductases. Imidazolplatin insignificantly inhibited NADPH-reductases. It is concluded that negligible inhibiting effect of imidazolplatin on microsomal NADPH-oxidoreductase allows us to consider, this platinum derivative as a promising compound for further experimental trials as anticancer drug with low toxic action on the normal tissues of an organism.

AB - The influence of platinum derivatives, cisplatin (cis-diamminedichloroplatinum) and imidazolplatin (cis-diimidazoledichloroplatinum) on the activity of rat liver microsomal NADPH-oxidoreductases was investigated in vitro using spectrophotometrical and chemiluminometrical methods. In the range of concentrations from 1 to 12 μM cisplatin inhibited NADPH-cytochrome c-reductase activity and NADPH/lucigenin-dependent chemiluminescence of microsomes. The 3 μM cisplatin decreased the NADPH-dependent reductase activity by 50%. At concentrations less than 2,5 mM sanazole (drug AK-2123) did not prevent the inhibiting influence of cisplatin on microsomal NADPH-dependent reductases. Imidazolplatin insignificantly inhibited NADPH-reductases. It is concluded that negligible inhibiting effect of imidazolplatin on microsomal NADPH-oxidoreductase allows us to consider, this platinum derivative as a promising compound for further experimental trials as anticancer drug with low toxic action on the normal tissues of an organism.

KW - Chemiluminescence

KW - Cisplatin

KW - Microsomes

KW - NAD(P)H-dependent reductases

KW - Platinum derivatives

UR - http://www.scopus.com/inward/record.url?scp=0442297366&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0442297366&partnerID=8YFLogxK

M3 - Article

VL - 47

SP - 380

EP - 381

JO - Voprosy Meditsinskoj Khimii

JF - Voprosy Meditsinskoj Khimii

SN - 0042-8809

IS - 4

ER -