Plasma mediated chlorhexidine immobilization onto polylactic acid surface via carbodiimide chemistry: Antibacterial and cytocompatibility assessment

Kadir Ozaltin, Antonio Di Martino, Zdenka Capakova, Marian Lehocky, Petr Humpolicek, Tomas Saha, Daniela Vesela, Miran Mozetic, Petr Saha

Research output: Contribution to journalArticlepeer-review

Abstract

The development of antibacterial materials has great importance in avoiding bacterial contamination and the risk of infection for implantable biomaterials. An antibacterial thin film coating on the surface via chemical bonding is a promising technique to keep native bulk material properties unchanged. However, most of the polymeric materials are chemically inert and highly hydrophobic, which makes chemical agent coating challenging Herein, immobilization of chlorhexidine, a broad-spectrum bactericidal cationic compound, onto the polylactic acid surface was performed in a multistep physicochemical method. Direct current plasma was used for surface functionalization, followed by carbodiimide chemistry to link the coupling reagents of N-(3-Dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDAC) and N-Hydroxysuccinimide (NHs) to create a free bonding site to anchor the chlorhexidine. Surface characterizations were performed by water contact angle test, X-ray photoelectron spectroscopy (XPS) and scanning electron microscope (SEM). X-ray photoelectron spectroscopy (XPS) and scanning electron microscope (SEM). The antibacterial activity was tested using Staphylococcus aureus and Escherichia coli. Finally, in vitro cytocompatibility of the samples was studied using primary mouse embryonic fibroblast cells. It was found that all samples were cytocompatible and the best antibacterial performance observed was the Chlorhexidine immobilized sample after NHs activation.

Original languageEnglish
Article number1201
JournalPolymers
Volume13
Issue number8
DOIs
Publication statusPublished - 2 Apr 2021

Keywords

  • Biomaterial associated infection
  • Chlorhexidine
  • Cyto-compatibility
  • Plasma treatment
  • Polylactic acid

ASJC Scopus subject areas

  • Chemistry(all)
  • Polymers and Plastics

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