Photosensitizer-loaded electrospun chitosan-based scaffolds for photodynamic therapy and tissue engineering

A. N. Severyukhina, N. V. Petrova, K. Smuda, G. S. Terentyuk, B. N. Klebtsov, R. Georgieva, H. Bäumler, D. A. Gorin

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Novel chitosan-based nanofibrous composite materials containing different amounts of the photosensitizer Photosens were obtained by electrospinning and were characterized by scanning electron microscopy and by confocal laser scanning microscopy. The release of Photosens from the materials was investigated in water and in phosphate-buffered saline. A noncancerous (MC3T3-E1 murine osteoblasts) and a cancerous [T-47D (mammary gland)] cell line were cultivated on Photosens-containing scaffolds, and cell growth and metabolic activity were examined by confocal laser scanning microscopy and by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphe-nyltetrazolium bromide assay, respectively. The viability of both cell lines on Photosens-containing fibers decreased in a spatial manner upon laser irradiation of an appropriate wavelength and power density. Interestingly, the noncancerous MC3T3-E1 cells grown on Photosens -containing scaffolds were less affected by the irradiation. We conclude that the Photosens-containing electrospun chitosan nanofibers described here are of potential interest for biomedical applications, particularly topical photodynamic therapy and tissue engineering.

Original languageEnglish
Pages (from-to)57-64
Number of pages8
JournalColloids and Surfaces B: Biointerfaces
Volume144
DOIs
Publication statusPublished - 1 Aug 2016
Externally publishedYes

Keywords

  • Electrospinning
  • Photoresponsive material
  • Photosensitizer
  • Spatially controlled cell proliferation
  • Tissue-engineering scaffold
  • Topical photodynamic therapy

ASJC Scopus subject areas

  • Biotechnology
  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Surfaces and Interfaces

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  • Cite this

    Severyukhina, A. N., Petrova, N. V., Smuda, K., Terentyuk, G. S., Klebtsov, B. N., Georgieva, R., Bäumler, H., & Gorin, D. A. (2016). Photosensitizer-loaded electrospun chitosan-based scaffolds for photodynamic therapy and tissue engineering. Colloids and Surfaces B: Biointerfaces, 144, 57-64. https://doi.org/10.1016/j.colsurfb.2016.03.081