Photosensitizer-loaded electrospun chitosan-based scaffolds for photodynamic therapy and tissue engineering

A. N. Severyukhina, N. V. Petrova, K. Smuda, G. S. Terentyuk, B. N. Klebtsov, R. Georgieva, H. Bäumler, D. A. Gorin

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Novel chitosan-based nanofibrous composite materials containing different amounts of the photosensitizer Photosens were obtained by electrospinning and were characterized by scanning electron microscopy and by confocal laser scanning microscopy. The release of Photosens from the materials was investigated in water and in phosphate-buffered saline. A noncancerous (MC3T3-E1 murine osteoblasts) and a cancerous [T-47D (mammary gland)] cell line were cultivated on Photosens-containing scaffolds, and cell growth and metabolic activity were examined by confocal laser scanning microscopy and by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphe-nyltetrazolium bromide assay, respectively. The viability of both cell lines on Photosens-containing fibers decreased in a spatial manner upon laser irradiation of an appropriate wavelength and power density. Interestingly, the noncancerous MC3T3-E1 cells grown on Photosens -containing scaffolds were less affected by the irradiation. We conclude that the Photosens-containing electrospun chitosan nanofibers described here are of potential interest for biomedical applications, particularly topical photodynamic therapy and tissue engineering.

Original languageEnglish
Pages (from-to)57-64
Number of pages8
JournalColloids and Surfaces B: Biointerfaces
Volume144
DOIs
Publication statusPublished - 1 Aug 2016
Externally publishedYes

Fingerprint

Photodynamic therapy
Photosensitizing Agents
Photosensitizers
tissue engineering
Photochemotherapy
Chitosan
Tissue Engineering
Scaffolds (biology)
Tissue engineering
therapy
Cells
engineering
cultured cells
Microscopic examination
mammary glands
microscopy
lasers
Scanning
osteoblasts
irradiation

Keywords

  • Electrospinning
  • Photoresponsive material
  • Photosensitizer
  • Spatially controlled cell proliferation
  • Tissue-engineering scaffold
  • Topical photodynamic therapy

ASJC Scopus subject areas

  • Biotechnology
  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Surfaces and Interfaces

Cite this

Severyukhina, A. N., Petrova, N. V., Smuda, K., Terentyuk, G. S., Klebtsov, B. N., Georgieva, R., ... Gorin, D. A. (2016). Photosensitizer-loaded electrospun chitosan-based scaffolds for photodynamic therapy and tissue engineering. Colloids and Surfaces B: Biointerfaces, 144, 57-64. https://doi.org/10.1016/j.colsurfb.2016.03.081

Photosensitizer-loaded electrospun chitosan-based scaffolds for photodynamic therapy and tissue engineering. / Severyukhina, A. N.; Petrova, N. V.; Smuda, K.; Terentyuk, G. S.; Klebtsov, B. N.; Georgieva, R.; Bäumler, H.; Gorin, D. A.

In: Colloids and Surfaces B: Biointerfaces, Vol. 144, 01.08.2016, p. 57-64.

Research output: Contribution to journalArticle

Severyukhina, AN, Petrova, NV, Smuda, K, Terentyuk, GS, Klebtsov, BN, Georgieva, R, Bäumler, H & Gorin, DA 2016, 'Photosensitizer-loaded electrospun chitosan-based scaffolds for photodynamic therapy and tissue engineering', Colloids and Surfaces B: Biointerfaces, vol. 144, pp. 57-64. https://doi.org/10.1016/j.colsurfb.2016.03.081
Severyukhina, A. N. ; Petrova, N. V. ; Smuda, K. ; Terentyuk, G. S. ; Klebtsov, B. N. ; Georgieva, R. ; Bäumler, H. ; Gorin, D. A. / Photosensitizer-loaded electrospun chitosan-based scaffolds for photodynamic therapy and tissue engineering. In: Colloids and Surfaces B: Biointerfaces. 2016 ; Vol. 144. pp. 57-64.
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