Pectin coatings on titanium alloy scaffolds produced by additive manufacturing: Promotion of human bone marrow stromal cell proliferation

Timothy E.L. Douglas, Ute Hempel, Jagoda Żydek, Alina Vladescu, Krzysztof Pietryga, Julia A.H. Kaeswurm, Maria Buchweitz, Roman A. Surmenev, Maria A. Surmeneva, Cosmin M. Cotrut, Andrey V. Koptyug, Elżbieta Pamuła

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Ti6Al4V is a popular biomaterial for load-bearing implants for bone contact, which can be fabricated by additive manufacturing technologies. Their long-term success depends on their stable anchoring in surrounding bone, which in turn depends on formation of new bone tissue on the implant surface, for which adhesion and proliferation of bone-forming cells is a pre-requisite. Hence, surface coatings which promote cell adhesion and proliferation are desirable. Here, Ti6Al4V discs prepared by additive manufacturing (EBM) were coated with layers of pectins, calcium-binding polysaccharides derived from citrus (C) and apple (A), which also contained alkaline phosphatase (ALP), the enzyme responsible for mineralization of bone tissue. Adhesion and proliferation of human bone marrow stromal cells (hBMSC) were assessed. Proliferation after 7 days was increased by A-ALP coatings and, in particular, by C-ALP coatings. Cell morphology was similar on coated and uncoated samples. In conclusion, ALP-loaded pectin coatings promote hBMSC adhesion and proliferation.

Original languageEnglish
Pages (from-to)225-228
Number of pages4
JournalMaterials Letters
Volume227
DOIs
Publication statusPublished - 15 Sep 2018

Keywords

  • Biomaterials
  • Biomimetic
  • Thin films

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

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