Passive and active targeting of quantum dots for whole-body fluorescence imaging of breast cancer xenografts

Irina V. Balalaeva, Tatiana A. Zdobnova, Irina V. Krutova, Anna A. Brilkina, Ekaterina N. Lebedenko, Sergey M. Deyev

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Far-red and near-infrared fluorescent quantum dots (QDs) have become advancing contrast agents for efficient whole-body tumor imaging. In this study, we investigated the possibility of the vital fluorescence imaging of tumor using two contrast agents on the basis of QDs: bioinert QDs coated with polyethyleneglycol and QDs bound with anti-HER2/neu scFv antibodies. HER2/neu-positive breast cancer tumor xenografts in nude mice were used as a model. It was shown that both bioinert and tumor-targeted QD probes can be successfully applied for visualization of the tumor using in vivo imaging method, but fluorescent signal of QD-4D5scFv in tumors was considerably stronger than that of QD-PEG.

Original languageEnglish
Pages (from-to)860-867
Number of pages8
JournalJournal of Biophotonics
Volume5
Issue number11-12
DOIs
Publication statusPublished - Nov 2012
Externally publishedYes

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Keywords

  • Breast cancer
  • Fluorescence
  • HER2/neu
  • Quantum dots
  • ScFv antibodies
  • Whole-body imaging

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)
  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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