Passive and active targeting of quantum dots for whole-body fluorescence imaging of breast cancer xenografts

Irina V. Balalaeva, Tatiana A. Zdobnova, Irina V. Krutova, Anna A. Brilkina, Ekaterina N. Lebedenko, Sergey M. Deyev

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Far-red and near-infrared fluorescent quantum dots (QDs) have become advancing contrast agents for efficient whole-body tumor imaging. In this study, we investigated the possibility of the vital fluorescence imaging of tumor using two contrast agents on the basis of QDs: bioinert QDs coated with polyethyleneglycol and QDs bound with anti-HER2/neu scFv antibodies. HER2/neu-positive breast cancer tumor xenografts in nude mice were used as a model. It was shown that both bioinert and tumor-targeted QD probes can be successfully applied for visualization of the tumor using in vivo imaging method, but fluorescent signal of QD-4D5scFv in tumors was considerably stronger than that of QD-PEG.

Original languageEnglish
Pages (from-to)860-867
Number of pages8
JournalJournal of Biophotonics
Volume5
Issue number11-12
DOIs
Publication statusPublished - Nov 2012
Externally publishedYes

Fingerprint

Whole Body Imaging
Quantum Dots
Optical Imaging
Heterografts
breast
Semiconductor quantum dots
cancer
Fluorescence
quantum dots
Tumors
Breast Neoplasms
tumors
Imaging techniques
fluorescence
Neoplasms
Contrast Media
Single-Chain Antibodies
antibodies
Nude Mice
Antibodies

Keywords

  • Breast cancer
  • Fluorescence
  • HER2/neu
  • Quantum dots
  • ScFv antibodies
  • Whole-body imaging

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)
  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Passive and active targeting of quantum dots for whole-body fluorescence imaging of breast cancer xenografts. / Balalaeva, Irina V.; Zdobnova, Tatiana A.; Krutova, Irina V.; Brilkina, Anna A.; Lebedenko, Ekaterina N.; Deyev, Sergey M.

In: Journal of Biophotonics, Vol. 5, No. 11-12, 11.2012, p. 860-867.

Research output: Contribution to journalArticle

Balalaeva, Irina V. ; Zdobnova, Tatiana A. ; Krutova, Irina V. ; Brilkina, Anna A. ; Lebedenko, Ekaterina N. ; Deyev, Sergey M. / Passive and active targeting of quantum dots for whole-body fluorescence imaging of breast cancer xenografts. In: Journal of Biophotonics. 2012 ; Vol. 5, No. 11-12. pp. 860-867.
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