Passive and active targeting of quantum dots for whole-body fluorescence imaging of breast cancer xenografts

Irina V. Balalaeva, Tatiana A. Zdobnova, Irina V. Krutova, Anna A. Brilkina, Ekaterina N. Lebedenko, Sergey M. Deyev

Research output: Contribution to journal › Article

Abstract

Far-red and near-infrared fluorescent quantum dots (QDs) have become advancing contrast agents for efficient whole-body tumor imaging. In this study, we investigated the possibility of the vital fluorescence imaging of tumor using two contrast agents on the basis of QDs: bioinert QDs coated with polyethyleneglycol and QDs bound with anti-HER2/neu scFv antibodies. HER2/neu-positive breast cancer tumor xenografts in nude mice were used as a model. It was shown that both bioinert and tumor-targeted QD probes can be successfully applied for visualization of the tumor using in vivo imaging method, but fluorescent signal of QD-4D5scFv in tumors was considerably stronger than that of QD-PEG.

Original language	English
Pages (from-to)	860-867
Number of pages	8
Journal	Journal of Biophotonics
Volume	5
Issue number	11-12
DOIs	https://doi.org/10.1002/jbio.201200080
Publication status	Published - Nov 2012
Externally published	Yes

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Keywords

Breast cancer
Fluorescence
HER2/neu
Quantum dots
ScFv antibodies
Whole-body imaging

ASJC Scopus subject areas

Materials Science(all)
Engineering(all)
Physics and Astronomy(all)
Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)

Cite this

Balalaeva, I. V., Zdobnova, T. A., Krutova, I. V., Brilkina, A. A., Lebedenko, E. N., & Deyev, S. M. (2012). Passive and active targeting of quantum dots for whole-body fluorescence imaging of breast cancer xenografts. Journal of Biophotonics, 5(11-12), 860-867. https://doi.org/10.1002/jbio.201200080

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abstract = "Far-red and near-infrared fluorescent quantum dots (QDs) have become advancing contrast agents for efficient whole-body tumor imaging. In this study, we investigated the possibility of the vital fluorescence imaging of tumor using two contrast agents on the basis of QDs: bioinert QDs coated with polyethyleneglycol and QDs bound with anti-HER2/neu scFv antibodies. HER2/neu-positive breast cancer tumor xenografts in nude mice were used as a model. It was shown that both bioinert and tumor-targeted QD probes can be successfully applied for visualization of the tumor using in vivo imaging method, but fluorescent signal of QD-4D5scFv in tumors was considerably stronger than that of QD-PEG.",

keywords = "Breast cancer, Fluorescence, HER2/neu, Quantum dots, ScFv antibodies, Whole-body imaging",

author = "Balalaeva, {Irina V.} and Zdobnova, {Tatiana A.} and Krutova, {Irina V.} and Brilkina, {Anna A.} and Lebedenko, {Ekaterina N.} and Deyev, {Sergey M.}",

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AU - Balalaeva, Irina V.

AU - Zdobnova, Tatiana A.

AU - Krutova, Irina V.

AU - Brilkina, Anna A.

AU - Lebedenko, Ekaterina N.

AU - Deyev, Sergey M.

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AB - Far-red and near-infrared fluorescent quantum dots (QDs) have become advancing contrast agents for efficient whole-body tumor imaging. In this study, we investigated the possibility of the vital fluorescence imaging of tumor using two contrast agents on the basis of QDs: bioinert QDs coated with polyethyleneglycol and QDs bound with anti-HER2/neu scFv antibodies. HER2/neu-positive breast cancer tumor xenografts in nude mice were used as a model. It was shown that both bioinert and tumor-targeted QD probes can be successfully applied for visualization of the tumor using in vivo imaging method, but fluorescent signal of QD-4D5scFv in tumors was considerably stronger than that of QD-PEG.

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Access to Document

10.1002/jbio.201200080