Abstract
Far-red and near-infrared fluorescent quantum dots (QDs) have become advancing contrast agents for efficient whole-body tumor imaging. In this study, we investigated the possibility of the vital fluorescence imaging of tumor using two contrast agents on the basis of QDs: bioinert QDs coated with polyethyleneglycol and QDs bound with anti-HER2/neu scFv antibodies. HER2/neu-positive breast cancer tumor xenografts in nude mice were used as a model. It was shown that both bioinert and tumor-targeted QD probes can be successfully applied for visualization of the tumor using in vivo imaging method, but fluorescent signal of QD-4D5scFv in tumors was considerably stronger than that of QD-PEG.
| Original language | English |
|---|---|
| Pages (from-to) | 860-867 |
| Number of pages | 8 |
| Journal | Journal of Biophotonics |
| Volume | 5 |
| Issue number | 11-12 |
| DOIs | |
| Publication status | Published - Nov 2012 |
| Externally published | Yes |
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Keywords
- Breast cancer
- Fluorescence
- HER2/neu
- Quantum dots
- ScFv antibodies
- Whole-body imaging
ASJC Scopus subject areas
- Materials Science(all)
- Engineering(all)
- Physics and Astronomy(all)
- Chemistry(all)
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Passive and active targeting of quantum dots for whole-body fluorescence imaging of breast cancer xenografts. / Balalaeva, Irina V.; Zdobnova, Tatiana A.; Krutova, Irina V.; Brilkina, Anna A.; Lebedenko, Ekaterina N.; Deyev, Sergey M.
In: Journal of Biophotonics, Vol. 5, No. 11-12, 11.2012, p. 860-867.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Passive and active targeting of quantum dots for whole-body fluorescence imaging of breast cancer xenografts
AU - Balalaeva, Irina V.
AU - Zdobnova, Tatiana A.
AU - Krutova, Irina V.
AU - Brilkina, Anna A.
AU - Lebedenko, Ekaterina N.
AU - Deyev, Sergey M.
PY - 2012/11
Y1 - 2012/11
N2 - Far-red and near-infrared fluorescent quantum dots (QDs) have become advancing contrast agents for efficient whole-body tumor imaging. In this study, we investigated the possibility of the vital fluorescence imaging of tumor using two contrast agents on the basis of QDs: bioinert QDs coated with polyethyleneglycol and QDs bound with anti-HER2/neu scFv antibodies. HER2/neu-positive breast cancer tumor xenografts in nude mice were used as a model. It was shown that both bioinert and tumor-targeted QD probes can be successfully applied for visualization of the tumor using in vivo imaging method, but fluorescent signal of QD-4D5scFv in tumors was considerably stronger than that of QD-PEG.
AB - Far-red and near-infrared fluorescent quantum dots (QDs) have become advancing contrast agents for efficient whole-body tumor imaging. In this study, we investigated the possibility of the vital fluorescence imaging of tumor using two contrast agents on the basis of QDs: bioinert QDs coated with polyethyleneglycol and QDs bound with anti-HER2/neu scFv antibodies. HER2/neu-positive breast cancer tumor xenografts in nude mice were used as a model. It was shown that both bioinert and tumor-targeted QD probes can be successfully applied for visualization of the tumor using in vivo imaging method, but fluorescent signal of QD-4D5scFv in tumors was considerably stronger than that of QD-PEG.
KW - Breast cancer
KW - Fluorescence
KW - HER2/neu
KW - Quantum dots
KW - ScFv antibodies
KW - Whole-body imaging
UR - http://www.scopus.com/inward/record.url?scp=84868691558&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84868691558&partnerID=8YFLogxK
U2 - 10.1002/jbio.201200080
DO - 10.1002/jbio.201200080
M3 - Article
VL - 5
SP - 860
EP - 867
JO - Journal of Biophotonics
JF - Journal of Biophotonics
SN - 1864-063X
IS - 11-12
ER -