Passive and active targeting of quantum dots for whole-body fluorescence imaging of breast cancer xenografts

Abstract

Far-red and near-infrared fluorescent quantum dots (QDs) have become advancing contrast agents for efficient whole-body tumor imaging. In this study, we investigated the possibility of the vital fluorescence imaging of tumor using two contrast agents on the basis of QDs: bioinert QDs coated with polyethyleneglycol and QDs bound with anti-HER2/neu scFv antibodies. HER2/neu-positive breast cancer tumor xenografts in nude mice were used as a model. It was shown that both bioinert and tumor-targeted QD probes can be successfully applied for visualization of the tumor using in vivo imaging method, but fluorescent signal of QD-4D5scFv in tumors was considerably stronger than that of QD-PEG.

Original language	English
Pages (from-to)	860-867
Number of pages	8
Journal	Journal of Biophotonics
Volume	5
Issue number	11-12
DOIs	https://doi.org/10.1002/jbio.201200080
Publication status	Published - Nov 2012
Externally published	Yes

Keywords

Breast cancer
Fluorescence
HER2/neu
Quantum dots
ScFv antibodies
Whole-body imaging

ASJC Scopus subject areas

Materials Science(all)
Engineering(all)
Physics and Astronomy(all)
Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)

Access to Document

10.1002/jbio.201200080

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Passive and active targeting of quantum dots for whole-body fluorescence imaging of breast cancer xenografts. / Balalaeva, Irina V.; Zdobnova, Tatiana A.; Krutova, Irina V.; Brilkina, Anna A.; Lebedenko, Ekaterina N.; Deyev, Sergey M.

In: Journal of Biophotonics, Vol. 5, No. 11-12, 11.2012, p. 860-867.

Research output: Contribution to journal › Article › peer-review

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abstract = "Far-red and near-infrared fluorescent quantum dots (QDs) have become advancing contrast agents for efficient whole-body tumor imaging. In this study, we investigated the possibility of the vital fluorescence imaging of tumor using two contrast agents on the basis of QDs: bioinert QDs coated with polyethyleneglycol and QDs bound with anti-HER2/neu scFv antibodies. HER2/neu-positive breast cancer tumor xenografts in nude mice were used as a model. It was shown that both bioinert and tumor-targeted QD probes can be successfully applied for visualization of the tumor using in vivo imaging method, but fluorescent signal of QD-4D5scFv in tumors was considerably stronger than that of QD-PEG.",

keywords = "Breast cancer, Fluorescence, HER2/neu, Quantum dots, ScFv antibodies, Whole-body imaging",

author = "Balalaeva, {Irina V.} and Zdobnova, {Tatiana A.} and Krutova, {Irina V.} and Brilkina, {Anna A.} and Lebedenko, {Ekaterina N.} and Deyev, {Sergey M.}",

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AU - Balalaeva, Irina V.

AU - Zdobnova, Tatiana A.

AU - Krutova, Irina V.

AU - Brilkina, Anna A.

AU - Lebedenko, Ekaterina N.

AU - Deyev, Sergey M.

PY - 2012/11

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AB - Far-red and near-infrared fluorescent quantum dots (QDs) have become advancing contrast agents for efficient whole-body tumor imaging. In this study, we investigated the possibility of the vital fluorescence imaging of tumor using two contrast agents on the basis of QDs: bioinert QDs coated with polyethyleneglycol and QDs bound with anti-HER2/neu scFv antibodies. HER2/neu-positive breast cancer tumor xenografts in nude mice were used as a model. It was shown that both bioinert and tumor-targeted QD probes can be successfully applied for visualization of the tumor using in vivo imaging method, but fluorescent signal of QD-4D5scFv in tumors was considerably stronger than that of QD-PEG.

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