Particle-based optical sensing of intracellular ions at the example of calcium - What are the experimental pitfalls?

Karsten Kantner, Sumaira Ashraf, Susana Carregal-Romero, Carolina Carrillo-Carrion, Mayeul Collot, Pablo Del Pino, Wolfram Heimbrodt, Dorleta Jimenez De Aberasturi, Uwe Kaiser, Lyubov I. Kazakova, Marco Lelle, Natalia Martinez De Baroja, Jose Maria Montenegro, Moritz Nazarenus, Beatriz Pelaz, Kalina Peneva, Pilar Rivera Gil, Nadeem Sabir, Lorenz Maximilian Schneider, Lyudmila I. ShabarchinaGleb B. Sukhorukov, Margarita Vazquez, Fang Yang, Wolfgang J. Parak

Research output: Contribution to journalReview article

21 Citations (Scopus)

Abstract

Colloidal particles with fluorescence read-out are commonly used as sensors for the quantitative determination of ions. Calcium, for example, is a biologically highly relevant ion in signaling, and thus knowledge of its spatio-temporal distribution inside cells would offer important experimental data. However, the use of particle-based intracellular sensors for ion detection is not straightforward. Important associated problems involve delivery and intracellular location of particlebased fluorophores, crosstalk of the fluorescence read-out with pH, and spectral overlap of the emission spectra of different fluorophores. These potential problems are outlined and discussed here with selected experimental examples. Potential solutions are discussed and form a guideline for particle-based intracellular imaging of ions.

Original languageEnglish
Pages (from-to)896-904
Number of pages9
JournalSmall
Volume11
Issue number8
DOIs
Publication statusPublished - 25 Feb 2015
Externally publishedYes

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Calcium
Ions
Fluorophores
Fluorescence
Sensors
Crosstalk
Guidelines
Imaging techniques

ASJC Scopus subject areas

  • Biomaterials
  • Engineering (miscellaneous)
  • Biotechnology
  • Medicine(all)

Cite this

Kantner, K., Ashraf, S., Carregal-Romero, S., Carrillo-Carrion, C., Collot, M., Del Pino, P., ... Parak, W. J. (2015). Particle-based optical sensing of intracellular ions at the example of calcium - What are the experimental pitfalls? Small, 11(8), 896-904. https://doi.org/10.1002/smll.201402110

Particle-based optical sensing of intracellular ions at the example of calcium - What are the experimental pitfalls? / Kantner, Karsten; Ashraf, Sumaira; Carregal-Romero, Susana; Carrillo-Carrion, Carolina; Collot, Mayeul; Del Pino, Pablo; Heimbrodt, Wolfram; De Aberasturi, Dorleta Jimenez; Kaiser, Uwe; Kazakova, Lyubov I.; Lelle, Marco; De Baroja, Natalia Martinez; Montenegro, Jose Maria; Nazarenus, Moritz; Pelaz, Beatriz; Peneva, Kalina; Gil, Pilar Rivera; Sabir, Nadeem; Schneider, Lorenz Maximilian; Shabarchina, Lyudmila I.; Sukhorukov, Gleb B.; Vazquez, Margarita; Yang, Fang; Parak, Wolfgang J.

In: Small, Vol. 11, No. 8, 25.02.2015, p. 896-904.

Research output: Contribution to journalReview article

Kantner, K, Ashraf, S, Carregal-Romero, S, Carrillo-Carrion, C, Collot, M, Del Pino, P, Heimbrodt, W, De Aberasturi, DJ, Kaiser, U, Kazakova, LI, Lelle, M, De Baroja, NM, Montenegro, JM, Nazarenus, M, Pelaz, B, Peneva, K, Gil, PR, Sabir, N, Schneider, LM, Shabarchina, LI, Sukhorukov, GB, Vazquez, M, Yang, F & Parak, WJ 2015, 'Particle-based optical sensing of intracellular ions at the example of calcium - What are the experimental pitfalls?', Small, vol. 11, no. 8, pp. 896-904. https://doi.org/10.1002/smll.201402110
Kantner K, Ashraf S, Carregal-Romero S, Carrillo-Carrion C, Collot M, Del Pino P et al. Particle-based optical sensing of intracellular ions at the example of calcium - What are the experimental pitfalls? Small. 2015 Feb 25;11(8):896-904. https://doi.org/10.1002/smll.201402110
Kantner, Karsten ; Ashraf, Sumaira ; Carregal-Romero, Susana ; Carrillo-Carrion, Carolina ; Collot, Mayeul ; Del Pino, Pablo ; Heimbrodt, Wolfram ; De Aberasturi, Dorleta Jimenez ; Kaiser, Uwe ; Kazakova, Lyubov I. ; Lelle, Marco ; De Baroja, Natalia Martinez ; Montenegro, Jose Maria ; Nazarenus, Moritz ; Pelaz, Beatriz ; Peneva, Kalina ; Gil, Pilar Rivera ; Sabir, Nadeem ; Schneider, Lorenz Maximilian ; Shabarchina, Lyudmila I. ; Sukhorukov, Gleb B. ; Vazquez, Margarita ; Yang, Fang ; Parak, Wolfgang J. / Particle-based optical sensing of intracellular ions at the example of calcium - What are the experimental pitfalls?. In: Small. 2015 ; Vol. 11, No. 8. pp. 896-904.
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AU - Del Pino, Pablo

AU - Heimbrodt, Wolfram

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AU - Gil, Pilar Rivera

AU - Sabir, Nadeem

AU - Schneider, Lorenz Maximilian

AU - Shabarchina, Lyudmila I.

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