Partially structured state of the functional VH domain of the mouse anti-ferritin antibody F11

Sergey P. Martsev, Anatoly P. Dubnovitsky, Oleg A. Stremovsky, Alexander A. Chumanevich, Yaroslav I. Tsybovsky, Zinaida I. Kravchuk, Sergey M. Deyev

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8 Citations (Scopus)

Abstract

An antibody combining site generally involves the two variable domains, VH from the heavy and VL from the light chain. We expressed the individual VH domain of the mouse anti-human ferritin monoclonal antibody F11. The loss of affinity was not dramatic (Ka=4.0×107 M-1 versus 8.6×108 M-1 for the parent antibody) and comparable to that previously observed for other VHs. However, the functional VH domain adopted a partially structured state with a significant amount of distorted secondary and compact yet greatly destabilized tertiary structures, as demonstrated by spectroscopic and calorimetric probes. These data provide the first description for a functional antibody domain that meets all the criteria of a partially structured state.

Original languageEnglish
Pages (from-to)177-182
Number of pages6
JournalFEBS Letters
Volume518
Issue number1-3
DOIs
Publication statusPublished - 8 May 2002
Externally publishedYes

Keywords

  • Antibody folding and stability
  • Antigen-binding
  • VH domain

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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    Martsev, S. P., Dubnovitsky, A. P., Stremovsky, O. A., Chumanevich, A. A., Tsybovsky, Y. I., Kravchuk, Z. I., & Deyev, S. M. (2002). Partially structured state of the functional VH domain of the mouse anti-ferritin antibody F11. FEBS Letters, 518(1-3), 177-182. https://doi.org/10.1016/S0014-5793(02)02696-0