Abstract
An antibody combining site generally involves the two variable domains, VH from the heavy and VL from the light chain. We expressed the individual VH domain of the mouse anti-human ferritin monoclonal antibody F11. The loss of affinity was not dramatic (Ka=4.0×107 M-1 versus 8.6×108 M-1 for the parent antibody) and comparable to that previously observed for other VHs. However, the functional VH domain adopted a partially structured state with a significant amount of distorted secondary and compact yet greatly destabilized tertiary structures, as demonstrated by spectroscopic and calorimetric probes. These data provide the first description for a functional antibody domain that meets all the criteria of a partially structured state.
| Original language | English |
|---|---|
| Pages (from-to) | 177-182 |
| Number of pages | 6 |
| Journal | FEBS Letters |
| Volume | 518 |
| Issue number | 1-3 |
| DOIs | |
| Publication status | Published - 8 May 2002 |
| Externally published | Yes |
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Keywords
- Antibody folding and stability
- Antigen-binding
- VH domain
ASJC Scopus subject areas
- Structural Biology
- Biophysics
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology
Cite this
Partially structured state of the functional VH domain of the mouse anti-ferritin antibody F11. / Martsev, Sergey P.; Dubnovitsky, Anatoly P.; Stremovsky, Oleg A.; Chumanevich, Alexander A.; Tsybovsky, Yaroslav I.; Kravchuk, Zinaida I.; Deyev, Sergey M.
In: FEBS Letters, Vol. 518, No. 1-3, 08.05.2002, p. 177-182.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Partially structured state of the functional VH domain of the mouse anti-ferritin antibody F11
AU - Martsev, Sergey P.
AU - Dubnovitsky, Anatoly P.
AU - Stremovsky, Oleg A.
AU - Chumanevich, Alexander A.
AU - Tsybovsky, Yaroslav I.
AU - Kravchuk, Zinaida I.
AU - Deyev, Sergey M.
PY - 2002/5/8
Y1 - 2002/5/8
N2 - An antibody combining site generally involves the two variable domains, VH from the heavy and VL from the light chain. We expressed the individual VH domain of the mouse anti-human ferritin monoclonal antibody F11. The loss of affinity was not dramatic (Ka=4.0×107 M-1 versus 8.6×108 M-1 for the parent antibody) and comparable to that previously observed for other VHs. However, the functional VH domain adopted a partially structured state with a significant amount of distorted secondary and compact yet greatly destabilized tertiary structures, as demonstrated by spectroscopic and calorimetric probes. These data provide the first description for a functional antibody domain that meets all the criteria of a partially structured state.
AB - An antibody combining site generally involves the two variable domains, VH from the heavy and VL from the light chain. We expressed the individual VH domain of the mouse anti-human ferritin monoclonal antibody F11. The loss of affinity was not dramatic (Ka=4.0×107 M-1 versus 8.6×108 M-1 for the parent antibody) and comparable to that previously observed for other VHs. However, the functional VH domain adopted a partially structured state with a significant amount of distorted secondary and compact yet greatly destabilized tertiary structures, as demonstrated by spectroscopic and calorimetric probes. These data provide the first description for a functional antibody domain that meets all the criteria of a partially structured state.
KW - Antibody folding and stability
KW - Antigen-binding
KW - VH domain
UR - http://www.scopus.com/inward/record.url?scp=0037042225&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037042225&partnerID=8YFLogxK
U2 - 10.1016/S0014-5793(02)02696-0
DO - 10.1016/S0014-5793(02)02696-0
M3 - Article
VL - 518
SP - 177
EP - 182
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1-3
ER -