Oxidative and Reductive Detection Modes for Determination of Nitrophenols by High-Performance Liquid Chromatography with Amperometric Detection at a Boron Doped Diamond Electrode

Jana Karaová, Jiří Barek, Karolina Schwarzová-Pecková

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

High performance liquid chromatography with electrochemical detection using a wall-jet arrangement with a working boron-doped diamond film electrode was used for the determination of 2-nitrophenol, 4-nitrophenol, and 2,4-dinitrophenol using reduction and oxidation modes. The optimal conditions for separation at a C18 reverse phase column (125 × 4 millimeters, 5 micrometers) and amperometric detection of these nitrophenols were determined. Acetate buffer (0.05 mole per liter pH 4.7/methanol 58/42, v/v) was chosen as the mobile phase for cathodic detection at the potential of −1.2 volts. The linear dynamic range was 2 × 10−6 to 1 × 10−4 mole per liter and the limits of detection were from 0.7 to 1.2 micromole per liter. For anodic detection, the mobile phase was 0.05 mole per liter phosphate buffer pH 6.75/methanol (65/35, v/v) at a detection potential of +1.3 volts. The linear dynamic range was from 2 × 10−6 to 1 × 10−4 mole per liter with limits of detection from 0.6 to 1.5 micromoles per liter. The method was successfully employed for direct determination of nitrophenols in drinking and river water with limits of detection between 0.7 and 1.8 micromoles per liter.

Original languageEnglish
Pages (from-to)66-79
Number of pages14
JournalAnalytical Letters
Volume49
Issue number1
DOIs
Publication statusPublished - 2 Jan 2016
Externally publishedYes

Fingerprint

Nitrophenols
Diamond
Boron
High performance liquid chromatography
Methanol
Limit of Detection
Diamonds
Buffers
Electrodes
High Pressure Liquid Chromatography
2,4-Dinitrophenol
Diamond films
Acetates
Rivers
Phosphates
Drinking Water
Oxidation
Water
2-nitrophenol
4-nitrophenol

Keywords

  • Amperometric detection
  • boron-doped diamond electrode
  • high-performance liquid chromatography
  • HPLC
  • nitrophenols
  • wall-jet detector

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Spectroscopy
  • Clinical Biochemistry
  • Biochemistry, medical
  • Electrochemistry

Cite this

Oxidative and Reductive Detection Modes for Determination of Nitrophenols by High-Performance Liquid Chromatography with Amperometric Detection at a Boron Doped Diamond Electrode. / Karaová, Jana; Barek, Jiří; Schwarzová-Pecková, Karolina.

In: Analytical Letters, Vol. 49, No. 1, 02.01.2016, p. 66-79.

Research output: Contribution to journalArticle

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