Osteoblastic differentiation of mesenchymal stem cells by mumie extract

Cho Rok Jung, Igor A. Schepetkin, Sang B. Woo, Andrey Ivanovich Khlebnikov, Byoung S. Kwon

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Abstract

Mumie, a plant humus matter from rocks, is known as anabolic and a stimulator of bone regeneration in the Russian and Indian systems of health and medicine. The water-soluble fraction of mumie from Uzbekistan was characterized using 1H NMR and infrared spectroscopic methods. The mumie extract has been investigated for its effect on osteoblastic differentiation in cell culture assays of human and murine mesenchymal stem cells. The calcium deposition and expression of alkaline phosphatase, osteocalcin, core binding factor 1 (Cbfa1), and ERK have been studied. During the 14-day assay period, human bone marrow mesenchymal stem cells (hMSCs) and human fetal osteoblasts cultured with mumie (3-5 μg/ml) underwent a dramatic change in cellular morphology, which was accompanied by a significant increase in alkaline phosphatase activity, calcium deposition, and osteocalcin expression. The expression of core binding factor 1 and ERK were enhanced in hMSCs and murine pluripotent mesenchymal precursor cell line C2C12. Dose-dependent decrease in TRAP-positive multinucleated cell formation from macrophage-like cells RAW 264.7 was observed with increasing concentration of mumie in the presence of RANKL (40 ng/ml) and PD98059 (10 μM), a specific inhibitor of ERK activity. The data suggest that mumie is a potent stimulator of osteoblastic differentiation of mesenchymal stem cells and inhibitor of osteoclastogenesis, hence it maybe of clinical benefit in the treatment for osteoporosis in human.

Original languageEnglish
Pages (from-to)122-133
Number of pages12
JournalDrug Development Research
Volume57
Issue number3
DOIs
Publication statusPublished - 1 Nov 2002
Externally publishedYes

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Keywords

  • Humic substances
  • Mesenchymal stem cells
  • Mumie
  • Osteoblasts
  • Osteoclasts

ASJC Scopus subject areas

  • Organic Chemistry
  • Drug Discovery
  • Pharmacology

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