Abstract
The use of organic-inorganic hybrid nanocarriers for controlled release of anticancer drugs has been gained a great interest, in particular, to improve the selectivity and efficacy of the drugs. In this study, iron oxide nanoparticles were prepared then surface modified via diazonium chemistry and coated with chitosan, and its derivative chitosan-grafted polylactic acid. The purpose was to increase the stability of the nanoparticles in physiological solution, heighten drug-loading capacity, prolong the release, reduce the initial burst effect and improve in vitro cytotoxicity of the model drug doxorubicin. The materials were characterized by DLS, ζ-potential, SEM, TGA, magnetization curves and release kinetics studies. Results confirmed the spherical shape, the presence of the coat and the advantages of using chitosan, particularly its amphiphilic derivative, as a coating agent, thereby surpassing the qualities of simple iron oxide nanoparticles. The coated nanoparticles exhibited great stability and high encapsulation efficiency for doxorubicin, at over 500 μg per mg of carrier. Moreover, the intensity of the initial burst was clearly diminished after coating, hence represents an advantage of using the hybrid system over simple iron oxide nanoparticles. Cytotoxicity studies demonstrate the increase in cytotoxicity of doxorubicin when loaded in nanoparticles, indirectly proving the role played by the carrier and its surface properties in cell uptake.
| Original language | English |
|---|---|
| Pages (from-to) | 380-390 |
| Number of pages | 11 |
| Journal | International Journal of Pharmaceutics |
| Volume | 526 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - 30 Jun 2017 |
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Keywords
- Chitosan
- Controlled release
- Doxorubicin
- Iron nanoparticles
- Polylactic acid
ASJC Scopus subject areas
- Pharmaceutical Science
Cite this
Organic-inorganic hybrid nanoparticles controlled delivery system for anticancer drugs. / Di Martino, Antonio; Guselnikova, Olga Andreevna; Trusova, Marina E.; Postnikov, Pavel S.; Sedlarik, Vladimir.
In: International Journal of Pharmaceutics, Vol. 526, No. 1-2, 30.06.2017, p. 380-390.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Organic-inorganic hybrid nanoparticles controlled delivery system for anticancer drugs
AU - Di Martino, Antonio
AU - Guselnikova, Olga Andreevna
AU - Trusova, Marina E.
AU - Postnikov, Pavel S.
AU - Sedlarik, Vladimir
PY - 2017/6/30
Y1 - 2017/6/30
N2 - The use of organic-inorganic hybrid nanocarriers for controlled release of anticancer drugs has been gained a great interest, in particular, to improve the selectivity and efficacy of the drugs. In this study, iron oxide nanoparticles were prepared then surface modified via diazonium chemistry and coated with chitosan, and its derivative chitosan-grafted polylactic acid. The purpose was to increase the stability of the nanoparticles in physiological solution, heighten drug-loading capacity, prolong the release, reduce the initial burst effect and improve in vitro cytotoxicity of the model drug doxorubicin. The materials were characterized by DLS, ζ-potential, SEM, TGA, magnetization curves and release kinetics studies. Results confirmed the spherical shape, the presence of the coat and the advantages of using chitosan, particularly its amphiphilic derivative, as a coating agent, thereby surpassing the qualities of simple iron oxide nanoparticles. The coated nanoparticles exhibited great stability and high encapsulation efficiency for doxorubicin, at over 500 μg per mg of carrier. Moreover, the intensity of the initial burst was clearly diminished after coating, hence represents an advantage of using the hybrid system over simple iron oxide nanoparticles. Cytotoxicity studies demonstrate the increase in cytotoxicity of doxorubicin when loaded in nanoparticles, indirectly proving the role played by the carrier and its surface properties in cell uptake.
AB - The use of organic-inorganic hybrid nanocarriers for controlled release of anticancer drugs has been gained a great interest, in particular, to improve the selectivity and efficacy of the drugs. In this study, iron oxide nanoparticles were prepared then surface modified via diazonium chemistry and coated with chitosan, and its derivative chitosan-grafted polylactic acid. The purpose was to increase the stability of the nanoparticles in physiological solution, heighten drug-loading capacity, prolong the release, reduce the initial burst effect and improve in vitro cytotoxicity of the model drug doxorubicin. The materials were characterized by DLS, ζ-potential, SEM, TGA, magnetization curves and release kinetics studies. Results confirmed the spherical shape, the presence of the coat and the advantages of using chitosan, particularly its amphiphilic derivative, as a coating agent, thereby surpassing the qualities of simple iron oxide nanoparticles. The coated nanoparticles exhibited great stability and high encapsulation efficiency for doxorubicin, at over 500 μg per mg of carrier. Moreover, the intensity of the initial burst was clearly diminished after coating, hence represents an advantage of using the hybrid system over simple iron oxide nanoparticles. Cytotoxicity studies demonstrate the increase in cytotoxicity of doxorubicin when loaded in nanoparticles, indirectly proving the role played by the carrier and its surface properties in cell uptake.
KW - Chitosan
KW - Controlled release
KW - Doxorubicin
KW - Iron nanoparticles
KW - Polylactic acid
UR - http://www.scopus.com/inward/record.url?scp=85019202796&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85019202796&partnerID=8YFLogxK
U2 - 10.1016/j.ijpharm.2017.04.061
DO - 10.1016/j.ijpharm.2017.04.061
M3 - Article
AN - SCOPUS:85019202796
VL - 526
SP - 380
EP - 390
JO - International Journal of Pharmaceutics
JF - International Journal of Pharmaceutics
SN - 0378-5173
IS - 1-2
ER -