Optimized indirect 76Br-bromination of antibodies using N-succinimidyl para-[76Br]bromobenzoate for radioimmuno PET

Abstract

Monoclonal antibody 38S1 was radiobrominated with the positron emitter 76Br (T_1/2 = 16.2 h). Indirect labeling was performed using N-succinimidyl para-(tri-methylstannyl)benzoate (SPMB) as the precursor molecule. SPMB was labeled using Chloramine-T yielding N-succinimidyl para-[76Br]bromobenzoate, which was then conjugated to the antibody. Optimization of the labeling conditions and further conjugation gave a total yield (mean ± max error) of 49 ± 2%. The immunoreactivity of the antibodies was retained after labeling. Thus, antibodies intended for positron emission tomography can be labeled with 76Br, which gives high yields and preserved immunoreactivity when using the SPMB technique described.

Original language	English
Pages (from-to)	837-843
Number of pages	7
Journal	Nuclear Medicine and Biology
Volume	27
Issue number	8
DOIs	https://doi.org/10.1016/S0969-8051(00)00153-0
Publication status	Published - 2000
Externally published	Yes

Keywords

Br
Indirect labeling
Monoclonal antibody
N-succinimidyl para-[Br]bromobenzoate
PET

ASJC Scopus subject areas

Molecular Medicine
Radiology Nuclear Medicine and imaging
Cancer Research

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@article{dc9b3c8a232f442596ed0a35882bd061,

title = "Optimized indirect 76Br-bromination of antibodies using N-succinimidyl para-[76Br]bromobenzoate for radioimmuno PET",

abstract = "Monoclonal antibody 38S1 was radiobrominated with the positron emitter 76Br (T1/2 = 16.2 h). Indirect labeling was performed using N-succinimidyl para-(tri-methylstannyl)benzoate (SPMB) as the precursor molecule. SPMB was labeled using Chloramine-T yielding N-succinimidyl para-[76Br]bromobenzoate, which was then conjugated to the antibody. Optimization of the labeling conditions and further conjugation gave a total yield (mean ± max error) of 49 ± 2%. The immunoreactivity of the antibodies was retained after labeling. Thus, antibodies intended for positron emission tomography can be labeled with 76Br, which gives high yields and preserved immunoreactivity when using the SPMB technique described.",

keywords = "Br, Indirect labeling, Monoclonal antibody, N-succinimidyl para-[Br]bromobenzoate, PET",

author = "Johanna H{\"o}glund and Vladimir Tolmachev and Anna Orlova and Hans Lundqvist and Anders Sundin",

note = "Funding Information: The authors would like to thank the staff at the Uppsala University PET Centre for their assistance with the radiobromine production. We also gratefully acknowledge The Svedberg Laboratory for allowing us to use their facilities and Pharmacia, Uppsala, Sweden, for supplying purified antibodies and antigens. This work was supported by the Swedish Cancer Foundation (grant no. 2971-B97-08XAB). Copyright: Copyright 2007 Elsevier B.V., All rights reserved.",

year = "2000",

doi = "10.1016/S0969-8051(00)00153-0",

language = "English",

volume = "27",

pages = "837--843",

journal = "Nuclear Medicine and Biology",

issn = "0969-8051",

publisher = "Elsevier Inc.",

number = "8",

}

TY - JOUR

T1 - Optimized indirect 76Br-bromination of antibodies using N-succinimidyl para-[76Br]bromobenzoate for radioimmuno PET

AU - Höglund, Johanna

AU - Tolmachev, Vladimir

AU - Orlova, Anna

AU - Lundqvist, Hans

AU - Sundin, Anders

N1 - Funding Information: The authors would like to thank the staff at the Uppsala University PET Centre for their assistance with the radiobromine production. We also gratefully acknowledge The Svedberg Laboratory for allowing us to use their facilities and Pharmacia, Uppsala, Sweden, for supplying purified antibodies and antigens. This work was supported by the Swedish Cancer Foundation (grant no. 2971-B97-08XAB). Copyright: Copyright 2007 Elsevier B.V., All rights reserved.

PY - 2000

Y1 - 2000

N2 - Monoclonal antibody 38S1 was radiobrominated with the positron emitter 76Br (T1/2 = 16.2 h). Indirect labeling was performed using N-succinimidyl para-(tri-methylstannyl)benzoate (SPMB) as the precursor molecule. SPMB was labeled using Chloramine-T yielding N-succinimidyl para-[76Br]bromobenzoate, which was then conjugated to the antibody. Optimization of the labeling conditions and further conjugation gave a total yield (mean ± max error) of 49 ± 2%. The immunoreactivity of the antibodies was retained after labeling. Thus, antibodies intended for positron emission tomography can be labeled with 76Br, which gives high yields and preserved immunoreactivity when using the SPMB technique described.

AB - Monoclonal antibody 38S1 was radiobrominated with the positron emitter 76Br (T1/2 = 16.2 h). Indirect labeling was performed using N-succinimidyl para-(tri-methylstannyl)benzoate (SPMB) as the precursor molecule. SPMB was labeled using Chloramine-T yielding N-succinimidyl para-[76Br]bromobenzoate, which was then conjugated to the antibody. Optimization of the labeling conditions and further conjugation gave a total yield (mean ± max error) of 49 ± 2%. The immunoreactivity of the antibodies was retained after labeling. Thus, antibodies intended for positron emission tomography can be labeled with 76Br, which gives high yields and preserved immunoreactivity when using the SPMB technique described.

KW - Br

KW - Indirect labeling

KW - Monoclonal antibody

KW - N-succinimidyl para-[Br]bromobenzoate

KW - PET

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U2 - 10.1016/S0969-8051(00)00153-0

DO - 10.1016/S0969-8051(00)00153-0

M3 - Article

C2 - 11150718

AN - SCOPUS:0034528045

VL - 27

SP - 837

EP - 843

JO - Nuclear Medicine and Biology

JF - Nuclear Medicine and Biology

SN - 0969-8051

IS - 8

ER -

Optimized indirect ⁷⁶Br-bromination of antibodies using N-succinimidyl para-[⁷⁶Br]bromobenzoate for radioimmuno PET

Abstract

Keywords

ASJC Scopus subject areas

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