Optimized indirect 76Br-bromination of antibodies using N-succinimidyl para-[76Br]bromobenzoate for radioimmuno PET

Johanna Höglund, Vladimir Tolmachev, Anna Orlova, Hans Lundqvist, Anders Sundin

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

Monoclonal antibody 38S1 was radiobrominated with the positron emitter 76Br (T1/2 = 16.2 h). Indirect labeling was performed using N-succinimidyl para-(tri-methylstannyl)benzoate (SPMB) as the precursor molecule. SPMB was labeled using Chloramine-T yielding N-succinimidyl para-[76Br]bromobenzoate, which was then conjugated to the antibody. Optimization of the labeling conditions and further conjugation gave a total yield (mean ± max error) of 49 ± 2%. The immunoreactivity of the antibodies was retained after labeling. Thus, antibodies intended for positron emission tomography can be labeled with 76Br, which gives high yields and preserved immunoreactivity when using the SPMB technique described.

Original languageEnglish
Pages (from-to)837-843
Number of pages7
JournalNuclear Medicine and Biology
Volume27
Issue number8
DOIs
Publication statusPublished - 2000
Externally publishedYes

Keywords

  • Br
  • Indirect labeling
  • Monoclonal antibody
  • N-succinimidyl para-[Br]bromobenzoate
  • PET

ASJC Scopus subject areas

  • Molecular Medicine
  • Radiology Nuclear Medicine and imaging
  • Cancer Research

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