Optimization of iodination of [125I]-N-succinimidyl-para-iodobenzoate using Chloramine-T for labeling of proteins

V. Tolmachev, A. Orlova, H. Lundqvist

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Generally, indirect radioiodination of proteins using N-succinimidyl-iodobenzoates preserve the biological function of the protein due the mild labeling conditions. However, a drawback is that the two-step procedure often gives a low overall labeling yield. For this reason the production of [125I] N-succinimidyl-para-iodobenzoate ([125I]SP1B), using Chloramine-T as an oxidant, was optimized regarding substrate, oxidant, reaction time, and volume of the reaction mixture and found to be 86 ± 6%. Produced [125I]SPIB was successfully used for the labeling of monoclonal antibodies.

Original languageEnglish
Pages (from-to)207-213
Number of pages7
JournalJournal of Radioanalytical and Nuclear Chemistry
Volume246
Issue number1
DOIs
Publication statusPublished - 2000
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Nuclear Energy and Engineering
  • Radiology Nuclear Medicine and imaging
  • Pollution
  • Spectroscopy
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

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