Generally, indirect radioiodination of proteins using N-succinimidyl-iodobenzoates preserve the biological function of the protein due the mild labeling conditions. However, a drawback is that the two-step procedure often gives a low overall labeling yield. For this reason the production of [125I] N-succinimidyl-para-iodobenzoate ([125I]SP1B), using Chloramine-T as an oxidant, was optimized regarding substrate, oxidant, reaction time, and volume of the reaction mixture and found to be 86 ± 6%. Produced [125I]SPIB was successfully used for the labeling of monoclonal antibodies.
ASJC Scopus subject areas
- Analytical Chemistry
- Nuclear Energy and Engineering
- Radiology Nuclear Medicine and imaging
- Public Health, Environmental and Occupational Health
- Health, Toxicology and Mutagenesis