On mechanism of antioxidant effect of fullerenols

A. S. Sachkova, E. S. Kovel, G. N. Churilov, O. A. Guseynov, A. A. Bondar, I. A. Dubinina, N. S. Kudryasheva

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Fullerenols are nanosized water-soluble polyhydroxylated derivatives of fullerenes, specific allotropic form of carbon, bioactive compounds and perspective pharmaceutical agents. Antioxidant activity of fullerenols was studied in model solutions of organic and inorganic toxicants of oxidative type – 1,4-benzoquinone and potassium ferricyanide. Two fullerenol preparations were tested: С60О2–4(ОН)20–24 and mixture of two types of fullerenols С60О2–4(ОН)20–2470О2–4(ОН)20–24. Bacteria-based and enzyme-based bioluminescent assays were used to evaluate a decrease in cellular and biochemical toxicities, respectively. Additionally, the enzyme-based assay was used for the direct monitoring of efficiency of the oxidative enzymatic processes. The bacteria-based and enzyme-based assays showed similar peculiarities of the detoxification processes: (1) ultralow concentrations of fullerenols were active (ca 10–17–10−4 and 10–17–10 5 g/L, respectively), (2) no monotonic dependence of detoxification efficiency on fullerenol concentrations was observed, and (3) detoxification of organic oxidizer solutions was more effective than that of the inorganic oxidizer. The antioxidant effect of highly diluted fullerenol solutions on bacterial cells was attributed to hormesis phenomenon; the detoxification was concerned with stimulation of adaptive cellular response under low-dose exposures. Sequence analysis of 16S ribosomal RNA was carried out; it did not reveal mutations in bacterial DNA. The suggestion was made that hydrophobic membrane-dependent processes are involved to the detoxifying mechanism. Catalytic activity of fullerenol (10 8 g/L) in NADH-dependent enzymatic reactions was demonstrated and supposed to contribute to adaptive bacterial response.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalBiochemistry and Biophysics Reports
Volume9
DOIs
Publication statusPublished - 1 Mar 2017

Fingerprint

Antioxidants
Detoxification
Assays
Enzyme Assays
Bacteria
Enzymes
Hormesis
16S Ribosomal RNA
Luminescent Measurements
Fullerenes
Bacterial DNA
fullerenol
NAD
Sequence Analysis
Toxicity
Catalyst activity
Carbon
Derivatives
Membranes
Mutation

Keywords

  • Antioxidant activity
  • Bacterial enzymes
  • Fullerenol
  • Hormesis
  • Luminous marine bacteria
  • Ultralow concentrations

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Sachkova, A. S., Kovel, E. S., Churilov, G. N., Guseynov, O. A., Bondar, A. A., Dubinina, I. A., & Kudryasheva, N. S. (2017). On mechanism of antioxidant effect of fullerenols. Biochemistry and Biophysics Reports, 9, 1-8. https://doi.org/10.1016/j.bbrep.2016.10.011

On mechanism of antioxidant effect of fullerenols. / Sachkova, A. S.; Kovel, E. S.; Churilov, G. N.; Guseynov, O. A.; Bondar, A. A.; Dubinina, I. A.; Kudryasheva, N. S.

In: Biochemistry and Biophysics Reports, Vol. 9, 01.03.2017, p. 1-8.

Research output: Contribution to journalArticle

Sachkova, AS, Kovel, ES, Churilov, GN, Guseynov, OA, Bondar, AA, Dubinina, IA & Kudryasheva, NS 2017, 'On mechanism of antioxidant effect of fullerenols', Biochemistry and Biophysics Reports, vol. 9, pp. 1-8. https://doi.org/10.1016/j.bbrep.2016.10.011
Sachkova, A. S. ; Kovel, E. S. ; Churilov, G. N. ; Guseynov, O. A. ; Bondar, A. A. ; Dubinina, I. A. ; Kudryasheva, N. S. / On mechanism of antioxidant effect of fullerenols. In: Biochemistry and Biophysics Reports. 2017 ; Vol. 9. pp. 1-8.
@article{87fc197e10ce4195b02dee8f3c46e0e8,
title = "On mechanism of antioxidant effect of fullerenols",
abstract = "Fullerenols are nanosized water-soluble polyhydroxylated derivatives of fullerenes, specific allotropic form of carbon, bioactive compounds and perspective pharmaceutical agents. Antioxidant activity of fullerenols was studied in model solutions of organic and inorganic toxicants of oxidative type – 1,4-benzoquinone and potassium ferricyanide. Two fullerenol preparations were tested: С60О2–4(ОН)20–24 and mixture of two types of fullerenols С60О2–4(ОН)20–24+С70О2–4(ОН)20–24. Bacteria-based and enzyme-based bioluminescent assays were used to evaluate a decrease in cellular and biochemical toxicities, respectively. Additionally, the enzyme-based assay was used for the direct monitoring of efficiency of the oxidative enzymatic processes. The bacteria-based and enzyme-based assays showed similar peculiarities of the detoxification processes: (1) ultralow concentrations of fullerenols were active (ca 10–17–10−4 and 10–17–10− 5 g/L, respectively), (2) no monotonic dependence of detoxification efficiency on fullerenol concentrations was observed, and (3) detoxification of organic oxidizer solutions was more effective than that of the inorganic oxidizer. The antioxidant effect of highly diluted fullerenol solutions on bacterial cells was attributed to hormesis phenomenon; the detoxification was concerned with stimulation of adaptive cellular response under low-dose exposures. Sequence analysis of 16S ribosomal RNA was carried out; it did not reveal mutations in bacterial DNA. The suggestion was made that hydrophobic membrane-dependent processes are involved to the detoxifying mechanism. Catalytic activity of fullerenol (10− 8 g/L) in NADH-dependent enzymatic reactions was demonstrated and supposed to contribute to adaptive bacterial response.",
keywords = "Antioxidant activity, Bacterial enzymes, Fullerenol, Hormesis, Luminous marine bacteria, Ultralow concentrations",
author = "Sachkova, {A. S.} and Kovel, {E. S.} and Churilov, {G. N.} and Guseynov, {O. A.} and Bondar, {A. A.} and Dubinina, {I. A.} and Kudryasheva, {N. S.}",
year = "2017",
month = "3",
day = "1",
doi = "10.1016/j.bbrep.2016.10.011",
language = "English",
volume = "9",
pages = "1--8",
journal = "Biochemistry and Biophysics Reports",
issn = "2405-5808",
publisher = "Elsevier BV",

}

TY - JOUR

T1 - On mechanism of antioxidant effect of fullerenols

AU - Sachkova, A. S.

AU - Kovel, E. S.

AU - Churilov, G. N.

AU - Guseynov, O. A.

AU - Bondar, A. A.

AU - Dubinina, I. A.

AU - Kudryasheva, N. S.

PY - 2017/3/1

Y1 - 2017/3/1

N2 - Fullerenols are nanosized water-soluble polyhydroxylated derivatives of fullerenes, specific allotropic form of carbon, bioactive compounds and perspective pharmaceutical agents. Antioxidant activity of fullerenols was studied in model solutions of organic and inorganic toxicants of oxidative type – 1,4-benzoquinone and potassium ferricyanide. Two fullerenol preparations were tested: С60О2–4(ОН)20–24 and mixture of two types of fullerenols С60О2–4(ОН)20–24+С70О2–4(ОН)20–24. Bacteria-based and enzyme-based bioluminescent assays were used to evaluate a decrease in cellular and biochemical toxicities, respectively. Additionally, the enzyme-based assay was used for the direct monitoring of efficiency of the oxidative enzymatic processes. The bacteria-based and enzyme-based assays showed similar peculiarities of the detoxification processes: (1) ultralow concentrations of fullerenols were active (ca 10–17–10−4 and 10–17–10− 5 g/L, respectively), (2) no monotonic dependence of detoxification efficiency on fullerenol concentrations was observed, and (3) detoxification of organic oxidizer solutions was more effective than that of the inorganic oxidizer. The antioxidant effect of highly diluted fullerenol solutions on bacterial cells was attributed to hormesis phenomenon; the detoxification was concerned with stimulation of adaptive cellular response under low-dose exposures. Sequence analysis of 16S ribosomal RNA was carried out; it did not reveal mutations in bacterial DNA. The suggestion was made that hydrophobic membrane-dependent processes are involved to the detoxifying mechanism. Catalytic activity of fullerenol (10− 8 g/L) in NADH-dependent enzymatic reactions was demonstrated and supposed to contribute to adaptive bacterial response.

AB - Fullerenols are nanosized water-soluble polyhydroxylated derivatives of fullerenes, specific allotropic form of carbon, bioactive compounds and perspective pharmaceutical agents. Antioxidant activity of fullerenols was studied in model solutions of organic and inorganic toxicants of oxidative type – 1,4-benzoquinone and potassium ferricyanide. Two fullerenol preparations were tested: С60О2–4(ОН)20–24 and mixture of two types of fullerenols С60О2–4(ОН)20–24+С70О2–4(ОН)20–24. Bacteria-based and enzyme-based bioluminescent assays were used to evaluate a decrease in cellular and biochemical toxicities, respectively. Additionally, the enzyme-based assay was used for the direct monitoring of efficiency of the oxidative enzymatic processes. The bacteria-based and enzyme-based assays showed similar peculiarities of the detoxification processes: (1) ultralow concentrations of fullerenols were active (ca 10–17–10−4 and 10–17–10− 5 g/L, respectively), (2) no monotonic dependence of detoxification efficiency on fullerenol concentrations was observed, and (3) detoxification of organic oxidizer solutions was more effective than that of the inorganic oxidizer. The antioxidant effect of highly diluted fullerenol solutions on bacterial cells was attributed to hormesis phenomenon; the detoxification was concerned with stimulation of adaptive cellular response under low-dose exposures. Sequence analysis of 16S ribosomal RNA was carried out; it did not reveal mutations in bacterial DNA. The suggestion was made that hydrophobic membrane-dependent processes are involved to the detoxifying mechanism. Catalytic activity of fullerenol (10− 8 g/L) in NADH-dependent enzymatic reactions was demonstrated and supposed to contribute to adaptive bacterial response.

KW - Antioxidant activity

KW - Bacterial enzymes

KW - Fullerenol

KW - Hormesis

KW - Luminous marine bacteria

KW - Ultralow concentrations

UR - http://www.scopus.com/inward/record.url?scp=84995653327&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84995653327&partnerID=8YFLogxK

U2 - 10.1016/j.bbrep.2016.10.011

DO - 10.1016/j.bbrep.2016.10.011

M3 - Article

VL - 9

SP - 1

EP - 8

JO - Biochemistry and Biophysics Reports

JF - Biochemistry and Biophysics Reports

SN - 2405-5808

ER -