There are many methods that allow detection of complex analytes such as soil contaminants and explosives, which can be dispersed in the various of liquid and solid. Nevertheless, it remains a challenge to create a labon-a-chip (LoC) platform for on-line detection of small analytes volumes (below the few femtoliters), with high sensitivity, specificity and reliability. It is expected that the combination of microfluidics and plasmonic will create a highly sensitive and affordable lab-on-a-chip platform for the solving of above mentioned challenge. The proposed approach will utilize the unique advantages of microfluidics that explore and uses unusual behavior of microscale-restricted liquids and surface-enhanced Raman analytical method, which is able to recognize the single molecule of the targeted analyte. In this work, we demonstrated the model of a microfluidic micromixer incorporating with a plasmon active substrate. The design and realization of proposed experimental concept include the solving of following tasks: (i) utilization of Comsol software for mathematical simulation of possible processes in microfluidic mixer; (II) preparation of microfluidic platform using 3D printing; (III) application of excimer UV laser large-scale patterning and local gold deposition for creation of plasmonic active area. Our LoC platform allows detection of model analyte (R6G) at concentrations down to 10 fM (10-15 mol·L-1). Proposed unique features are the key to new scientific experiments and innovations in the field of labon-a-chip devices and analytical approaches.